2021
EpoR stimulates rapid cycling and larger red cells during mouse and human erythropoiesis
Hidalgo D, Bejder J, Pop R, Gellatly K, Hwang Y, Maxwell Scalf S, Eastman AE, Chen JJ, Zhu LJ, Heuberger JAAC, Guo S, Koury MJ, Nordsborg NB, Socolovsky M. EpoR stimulates rapid cycling and larger red cells during mouse and human erythropoiesis. Nature Communications 2021, 12: 7334. PMID: 34921133, PMCID: PMC8683474, DOI: 10.1038/s41467-021-27562-4.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsAntigens, CDBcl-X ProteinCD4 AntigensCell CycleCell DifferentiationCell NucleusCell SizeCell SurvivalCyclin-Dependent Kinase Inhibitor p27Embryo, MammalianErythroblastsErythrocytesErythropoiesisErythropoietinFemaleFetusHealthy VolunteersHumansIronLiverMaleMice, Inbred C57BLModels, BiologicalProtein Serine-Threonine KinasesReceptors, ErythropoietinReceptors, TransferrinReticulocytesSignal TransductionConceptsCell size regulationCell sizeSequential cell divisionsEpoR functionErythroblast survivalMouse erythroblastsCell divisionSize regulationHuman erythropoiesisErythropoietin receptorCell cycleEpoRHypoxic stressRed cell sizeHigh erythropoietinLarger red cellsWild-type miceCyclingErythroblastsRegulationHigher EPO levelsMiceRed cellsSurvivalErythropoiesisEpor Stimulates Rapid Cycling and Larger Red Cells during Mouse and Human Erythropoiesis
Hidalgo D, Bejder J, Pop R, Gellatly K, Hwang Y, Scalf S, Eastman A, Chen J, Zhu L, Heuberger J, Guo S, Koury M, Nordsborg N, Socolovsky M. Epor Stimulates Rapid Cycling and Larger Red Cells during Mouse and Human Erythropoiesis. Blood 2021, 138: 852. DOI: 10.1182/blood-2021-154403.Peer-Reviewed Original ResearchErythroid terminal differentiationCell divisionCell cycleWild-type erythroblastsAnti-apoptotic protein BclCell sizeLive-cell reporterCell cycle speedNon-redundant functionsLarger red cellsFetal liver progenitorsTransferrin receptorEIF2α kinaseEpoR functionErythroblast survivalGenetic systemProtein BclHuman erythropoiesisNegative regulatorSurvival signalsTerminal differentiationEffects of EpoEpoRMouse EpoRP27 Kip1
2020
Novel Fluorescent Timer Tool Enables Characterization of Erythropoietic Differentiation Based on Differential Cell Cycling Speeds
Modepalli S, Eastman A, Shaw C, Guo S, Hattangadi S, Kupfer G. Novel Fluorescent Timer Tool Enables Characterization of Erythropoietic Differentiation Based on Differential Cell Cycling Speeds. Blood 2020, 136: 27-28. DOI: 10.1182/blood-2020-141666.Peer-Reviewed Original ResearchCell cycle speedCell divisionErythroid differentiationFusion proteinOrthochromatic erythroblastsLive-cell reporterStress erythropoiesisFluorescent timer proteinsAvailable proteomic dataSubsequent cell divisionRapid cell divisionUpregulation of genesCell cycle dynamicsErythroid cell proliferationSingle-cell levelRed fluorescent proteinFaster cycling cellsCell cycle behaviorFT proteinPhosphoproteomic investigationsDiscrete molecular targetsInducible promoterProteomic dataActive lociStress hematopoiesis