2014
Bacterial Whole-Genome Sequencing Revisited: Portable, Scalable, and Standardized Analysis for Typing and Detection of Virulence and Antibiotic Resistance Genes
Leopold S, Goering R, Witten A, Harmsen D, Mellmann A. Bacterial Whole-Genome Sequencing Revisited: Portable, Scalable, and Standardized Analysis for Typing and Detection of Virulence and Antibiotic Resistance Genes. Journal Of Clinical Microbiology 2014, 52: 2365-2370. PMID: 24759713, PMCID: PMC4097726, DOI: 10.1128/jcm.00262-14.Peer-Reviewed Original ResearchConceptsIntensive care unitMethicillin-resistant Staphylococcus aureusEffective infection controlInfection controlEpidemiological investigationsWhole-genome sequencingAntibiotic resistanceMultidrug-resistant nosocomial pathogenAntibiotic resistance profilesToxin gene profilesMRSA casesMRSA clustersCare unitRegular screeningNosocomial infectionsDetection of virulenceMajor burdenNosocomial pathogenS. aureus proteinSusceptibility profilesResistance profilesClonal clustersWGS resultsTransmission eventsBacterial whole-genome sequencing
2013
Phylogeny and phenotypes of clinical and environmental Shiga toxin‐producing Escherichia coli O174
Zhang W, Nadirk J, Kossow A, Bielaszewska M, Leopold S, Witten A, Fruth A, Karch H, Ammon A, Mellmann A. Phylogeny and phenotypes of clinical and environmental Shiga toxin‐producing Escherichia coli O174. Environmental Microbiology 2013, 16: 963-976. PMID: 24034719, DOI: 10.1111/1462-2920.12234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Bacterial AgentsBacterial AdhesionCattleCell LineChlorocebus aethiopsDrug Resistance, Multiple, BacterialEscherichia coli ProteinsGenotypeHumansIntestinal MucosaMolecular Sequence DataMultilocus Sequence TypingPhenotypePhylogenyShiga ToxinShiga-Toxigenic Escherichia coliVero CellsConceptsDifferent clinical outcomesHaemolytic uremic syndromeMultilocus sequence typingClinical outcomesUremic syndromeStx genotypesH antigensShiga toxinO174Animal reservoirsSequence typingIntimin geneDifferent serotypesVirulence factorsAdherence capacitySerotypesNegative STECSTECSequence typesPhenotypic heterogeneityNon-motile isolatesToxinIsolate originVirulence lociDifferent genotypes
2011
Obscured phylogeny and possible recombinational dormancy in Escherichia coli
Leopold S, Sawyer S, Whittam T, Tarr P. Obscured phylogeny and possible recombinational dormancy in Escherichia coli. BMC Ecology And Evolution 2011, 11: 183. PMID: 21708031, PMCID: PMC3152902, DOI: 10.1186/1471-2148-11-183.Peer-Reviewed Original ResearchConceptsComplex recombination historyDefinition of speciesE. coliSegments of chromosomesPhylogenetic structureSingle nucleotide polymorphismsNew speciesGenetic materialE. coli groupEscherichia coliNucleotide polymorphismsRepresentative strainsPhylogenySpeciesColiChromosomesRecombination historyDormancyOrganismsColi groupBiologyDNARecombinationPolymorphismStrains
2009
A precise reconstruction of the emergence and constrained radiations of Escherichia coli O157 portrayed by backbone concatenomic analysis
Leopold S, Magrini V, Holt N, Shaikh N, Mardis E, Cagno J, Ogura Y, Iguchi A, Hayashi T, Mellmann A, Karch H, Besser T, Sawyer S, Whittam T, Tarr P. A precise reconstruction of the emergence and constrained radiations of Escherichia coli O157 portrayed by backbone concatenomic analysis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 8713-8718. PMID: 19439656, PMCID: PMC2689004, DOI: 10.1073/pnas.0812949106.Peer-Reviewed Original ResearchConceptsSingle nucleotide polymorphismsEffective population sizeSynonymous single nucleotide polymorphismsClusters of organismsGene clusterSpecies evolutionGenome regionsEvolutionary dynamicsPathogenic Escherichia coli O157Selective advantageEscherichia coli O157Related pathogensPopulation sizeNucleotide polymorphismsTenuous survivalPathogenic O157Coli O157OrganismsFalse associationsPathogensORFH7Backbone regionO157Mutations