2014
Bacterial Whole-Genome Sequencing Revisited: Portable, Scalable, and Standardized Analysis for Typing and Detection of Virulence and Antibiotic Resistance Genes
Leopold S, Goering R, Witten A, Harmsen D, Mellmann A. Bacterial Whole-Genome Sequencing Revisited: Portable, Scalable, and Standardized Analysis for Typing and Detection of Virulence and Antibiotic Resistance Genes. Journal Of Clinical Microbiology 2014, 52: 2365-2370. PMID: 24759713, PMCID: PMC4097726, DOI: 10.1128/jcm.00262-14.Peer-Reviewed Original ResearchConceptsIntensive care unitMethicillin-resistant Staphylococcus aureusEffective infection controlInfection controlEpidemiological investigationsWhole-genome sequencingAntibiotic resistanceMultidrug-resistant nosocomial pathogenAntibiotic resistance profilesToxin gene profilesMRSA casesMRSA clustersCare unitRegular screeningNosocomial infectionsDetection of virulenceMajor burdenNosocomial pathogenS. aureus proteinSusceptibility profilesResistance profilesClonal clustersWGS resultsTransmission eventsBacterial whole-genome sequencing
2013
Evolution of Enterohemorrhagic Escherichia coli O26 Based on Single-Nucleotide Polymorphisms
Bletz S, Bielaszewska M, Leopold S, Köck R, Witten A, Schuldes J, Zhang W, Karch H, Mellmann A. Evolution of Enterohemorrhagic Escherichia coli O26 Based on Single-Nucleotide Polymorphisms. Genome Biology And Evolution 2013, 5: 1807-1816. PMID: 24105689, PMCID: PMC3814194, DOI: 10.1093/gbe/evt136.Peer-Reviewed Original ResearchLineage and Genogroup-Defining Single Nucleotide Polymorphisms of Escherichia coli O157:H7
Jung W, Bono J, Clawson M, Leopold S, Shringi S, Besser T. Lineage and Genogroup-Defining Single Nucleotide Polymorphisms of Escherichia coli O157:H7. Applied And Environmental Microbiology 2013, 79: 7036-7041. PMID: 24014531, PMCID: PMC3811523, DOI: 10.1128/aem.02173-13.Peer-Reviewed Original ResearchConceptsSingle nucleotide polymorphismsNew sequencing dataNucleotide polymorphismsSequencing dataImportant reservoir hostsLineagesE. coli O157Typing panelZoonotic human pathogenColi O157H7 genotypesHuman pathogensRelevant lineagesLineage groupsReservoir hostsHuman clinical isolatesCattle isolatesBovine sourcesSNPsEscherichia coli O157IsolatesPolymorphismStx genesGenogroupsH7Phylogeny and phenotypes of clinical and environmental Shiga toxin‐producing Escherichia coli O174
Zhang W, Nadirk J, Kossow A, Bielaszewska M, Leopold S, Witten A, Fruth A, Karch H, Ammon A, Mellmann A. Phylogeny and phenotypes of clinical and environmental Shiga toxin‐producing Escherichia coli O174. Environmental Microbiology 2013, 16: 963-976. PMID: 24034719, DOI: 10.1111/1462-2920.12234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Bacterial AgentsBacterial AdhesionCattleCell LineChlorocebus aethiopsDrug Resistance, Multiple, BacterialEscherichia coli ProteinsGenotypeHumansIntestinal MucosaMolecular Sequence DataMultilocus Sequence TypingPhenotypePhylogenyShiga ToxinShiga-Toxigenic Escherichia coliVero CellsConceptsDifferent clinical outcomesHaemolytic uremic syndromeMultilocus sequence typingClinical outcomesUremic syndromeStx genotypesH antigensShiga toxinO174Animal reservoirsSequence typingIntimin geneDifferent serotypesVirulence factorsAdherence capacitySerotypesNegative STECSTECSequence typesPhenotypic heterogeneityNon-motile isolatesToxinIsolate originVirulence lociDifferent genotypes
2012
Identification of Intermediate in Evolutionary Model of Enterohemorrhagic Escherichia coli O157 - Volume 18, Number 4—April 2012 - Emerging Infectious Diseases journal - CDC
Jenke C, Leopold S, Weniger T, Rothgänger J, Harmsen D, Karch H, Mellmann A. Identification of Intermediate in Evolutionary Model of Enterohemorrhagic Escherichia coli O157 - Volume 18, Number 4—April 2012 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2012, 18: 582-588. PMID: 22469031, PMCID: PMC3309690, DOI: 10.3201/eid1804.111414.Peer-Reviewed Original ResearchConceptsH7/HEHEC O157Partial open reading frameEvolutionary modelsOpen reading frameStepwise evolutionary modelSingle nucleotide polymorphism analysisEnterohemorrhagic Escherichia coli O157Nucleotide polymorphism analysisGenomic regionsSingle nucleotide polymorphismsReading frameHuman pathogensNucleotide polymorphismsCurrent evolutionary modelsNonhuman reservoirsPolymorphism analysisEscherichia coli O157Animal originHypothetical intermediateH isolatesColi O157O157Intermediate positionIntermediates
2011
Intestinal damage in enterohemorrhagic Escherichia coli infection
Békássy Z, Calderon Toledo C, Leoj G, Kristoffersson A, Leopold S, Perez M, Karpman D. Intestinal damage in enterohemorrhagic Escherichia coli infection. Pediatric Nephrology 2011, 26: 2059-2071. PMID: 20809220, DOI: 10.1007/s00467-010-1616-9.Peer-Reviewed Original ResearchConceptsEnterohemorrhagic Escherichia coli infectionEscherichia coli infectionTUNEL-positive cellsIntestinal injuryIntestinal damageColi infectionSevere symptomsType III secretion effectorsAbundant TUNEL-positive cellsC3H/HeN micePresence of Stx2Mucosal cell deathMore TUNEL-positive cellsShiga toxin 2Fecal bacterial countsBacterial virulence factorsPresence of intiminSigmoid colonHeN miceDistal colonEHEC infectionMore symptomsToxin 2Strain 87Symptoms
2010
Further evidence of constrained radiation in the evolution of pathogenic Escherichia coli O157:H7
Leopold S, Shaikh N, Tarr P. Further evidence of constrained radiation in the evolution of pathogenic Escherichia coli O157:H7. Infection Genetics And Evolution 2010, 10: 1282-1285. PMID: 20691811, PMCID: PMC3742067, DOI: 10.1016/j.meegid.2010.07.021.Peer-Reviewed Original ResearchMeSH KeywordsBacterial Typing TechniquesBase SequenceBiological EvolutionDNA, BacterialEscherichia coliEscherichia coli O157Escherichia coli ProteinsEvolution, MolecularGene ExpressionGenetic VariationGenome, BacterialGenomicsGenotypeHumansMutationO AntigensPolymerase Chain ReactionPolymorphism, Single NucleotideRecombination, GeneticSequence Analysis, DNAShiga ToxinsVirulence