2020
Peri‐implantation cytokine profile differs between singleton and twin IVF pregnancies
Simpson S, Kaislasuo J, Peng G, Aldo P, Paidas M, Guller S, Mor G, Pal L. Peri‐implantation cytokine profile differs between singleton and twin IVF pregnancies. American Journal Of Reproductive Immunology 2020, 85: e13348. PMID: 32946159, PMCID: PMC8221280, DOI: 10.1111/aji.13348.Peer-Reviewed Original ResearchConceptsMaternal serum levelsSerum levelsSingleton gestationsIL-10Twin pregnanciesC motif chemokine ligand 10General immune reactionTwin IVF pregnanciesProspective longitudinal cohortSerum cytokine concentrationsAcademic fertility centerSerial serum samplesChemokine ligand 10Implantation phaseAnti-angiogenic roleSingleton conceptionsCXCL10 levelsIVF pregnanciesIVF-ETCytokine concentrationsInterleukin-10Fertility centerSerum concentrationsTumor necrosisLongitudinal cohort
2019
Thermal stability of cytokines: A review
Simpson S, Kaislasuo J, Guller S, Pal L. Thermal stability of cytokines: A review. Cytokine 2019, 125: 154829. PMID: 31472404, DOI: 10.1016/j.cyto.2019.154829.Peer-Reviewed Original Research
2014
Trophoblast Induces Monocyte Differentiation Into CD14+/CD16+ Macrophages
Aldo PB, Racicot K, Craviero V, Guller S, Romero R, Mor G. Trophoblast Induces Monocyte Differentiation Into CD14+/CD16+ Macrophages. American Journal Of Reproductive Immunology 2014, 72: 270-284. PMID: 24995492, PMCID: PMC4230492, DOI: 10.1111/aji.12288.Peer-Reviewed Original ResearchConceptsUnique macrophage phenotypeMacrophage phenotypeMonocyte differentiationFirst trimester trophoblast cellsPeripheral blood monocytesPhenotype of macrophagesCD14 surface expressionNormal deciduaHealthy pregnancyIL-10Chemokine productionEarly pregnancyIP-10Monocyte exposureIL-1BBlood monocytesDecidual phenotypePhagocytic capacityTrophoblast cellsFlow cytometryMorphologic changesMacrophagesMonocytesSurface expressionPlacenta developmentSingle- and double-stranded viral RNA generate distinct cytokine and antiviral responses in human fetal membranes
Bakaysa SL, Potter JA, Hoang M, Han CS, Guller S, Norwitz ER, Abrahams VM. Single- and double-stranded viral RNA generate distinct cytokine and antiviral responses in human fetal membranes. Molecular Human Reproduction 2014, 20: 701-708. PMID: 24723465, PMCID: PMC4072183, DOI: 10.1093/molehr/gau028.Peer-Reviewed Original ResearchConceptsHuman fetal membranesToll-like receptorsMIP-1βMIP-1αFetal membranesAntiviral responseIL-1βIL-6IL-2Viral infectionG-CSFCytokine/chemokine profilesViral ssRNAAdverse pregnancy outcomesMaternal-fetal interfaceInfection-associated inflammationMCP-1 secretionViral dsRNA analogueTNF-α productionMIP-1α secretionTLR adapter proteinsExpression of IFNβPregnancy outcomesCytokine profileChemokine profilesHuman Fetal Membranes Generate Distinct Cytokine Profiles in Response to Bacterial Toll-Like Receptor and Nod-Like Receptor Agonists1
Hoang M, Potter JA, Gysler SM, Han CS, Guller S, Norwitz ER, Abrahams VM. Human Fetal Membranes Generate Distinct Cytokine Profiles in Response to Bacterial Toll-Like Receptor and Nod-Like Receptor Agonists1. Biology Of Reproduction 2014, 90: 39, 1-9. PMID: 24429216, PMCID: PMC4076407, DOI: 10.1095/biolreprod.113.115428.Peer-Reviewed Original ResearchConceptsBacterial toll-like receptorsToll-like receptorsPathogen-associated molecular patternsInnate immune pattern recognition receptorsNOD-like receptorsPattern recognition receptorsImmune pattern recognition receptorsCytokine profileIL1B secretionFetal membranesCpG ODNCaspase-1Subsequent preterm birthInflammatory cytokine profilePreterm premature ruptureDistinct cytokine profilesMaternal-fetal interfaceInfection-associated inflammationMild stimulatory effectNLR agonistsPreterm birthPremature ruptureProinflammatory cytokinesCytokine productionMCP-1
2013
Effect of Culture Conditions on the Phenotype of THP‐1 Monocyte Cell Line
Aldo PB, Craveiro V, Guller S, Mor G. Effect of Culture Conditions on the Phenotype of THP‐1 Monocyte Cell Line. American Journal Of Reproductive Immunology 2013, 70: 80-86. PMID: 23621670, PMCID: PMC3703650, DOI: 10.1111/aji.12129.Peer-Reviewed Original Research
2009
The Isolation and Characterization of a Novel Telomerase Immortalized First Trimester Trophoblast Cell Line, Swan 71
Straszewski-Chavez SL, Abrahams VM, Alvero AB, Aldo PB, Ma Y, Guller S, Romero R, Mor G. The Isolation and Characterization of a Novel Telomerase Immortalized First Trimester Trophoblast Cell Line, Swan 71. Placenta 2009, 30: 939-948. PMID: 19766308, PMCID: PMC2784169, DOI: 10.1016/j.placenta.2009.08.007.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CDAntigens, Differentiation, MyelomonocyticApoptosisCell LineChorionic GonadotropinCytokinesFemaleFibronectinsHistocompatibility Antigens Class IHLA AntigensHLA-G AntigensHumansIntercellular Signaling Peptides and ProteinsKeratin-7Leukocyte Common AntigensPregnancyPregnancy Trimester, FirstTelomeraseThy-1 AntigensTrophoblastsVimentinConceptsFirst trimester trophoblast cell lineSwan 71 cellsTrophoblast cell lineFirst trimester trophoblast cellsFibroblast-specific antigenHuman chorionic gonadotrophinPrimary trophoblast cellsTrophoblast cellsHuman telomerase reverse transcriptaseFetal fibronectinCell linesCD90/ThySwan 71Growth factor profileTNF-alpha-induced apoptosisChorionic gonadotrophinCytokeratin 7Specific antigenTelomerase reverse transcriptaseNormal placentaFactor profileKaryotypic abnormalitiesPatient samplesPrimary human cellsAdequate cell numbersORIGINAL ARTICLE: Regulation of Nod1 and Nod2 in First Trimester Trophoblast Cells
Mulla MJ, Yu AG, Cardenas I, Guller S, Panda B, Abrahams VM. ORIGINAL ARTICLE: Regulation of Nod1 and Nod2 in First Trimester Trophoblast Cells. American Journal Of Reproductive Immunology 2009, 61: 294-302. PMID: 19260860, DOI: 10.1111/j.1600-0897.2009.00694.x.Peer-Reviewed Original ResearchMeSH KeywordsApoptosisBenzamidesCells, CulturedCytokinesDiaminopimelic AcidFemaleGene Expression Regulation, DevelopmentalHumansLipopolysaccharidesNF-kappa BNod1 Signaling Adaptor ProteinNod2 Signaling Adaptor ProteinPregnancyPregnancy Trimester, FirstSequence DeletionSignal TransductionThiazolesToll-Like Receptor 4TransgenesTrophoblastsConceptsFirst trimester trophoblast cellsTrophoblast cellsExpression of NOD1Cytoplasmic pattern recognition receptorsProduction of cytokinesNOD2 mRNA expressionPattern recognition receptorsNOD2 expressionCytokine responsesInflammatory responseNOD2 activationNOD1 activationNFkappaB inhibitorRecognition receptorsNOD1Nod1 stimulationNOD2Bacterial lipopolysaccharideMRNA expressionTLR4LipopolysaccharideNFkappaB pathwayRT-PCRBacterial peptidesTrophoblast
2005
Alterations in Syncytiotrophoblast Cytokine Expression Following Treatment with Lipopolysaccharide
Ma Y, Mor G, Abrahams VM, Buhimschi IA, Buhimschi CS, Guller S. Alterations in Syncytiotrophoblast Cytokine Expression Following Treatment with Lipopolysaccharide. American Journal Of Reproductive Immunology 2005, 55: 12-18. PMID: 16364007, DOI: 10.1111/j.1600-0897.2005.00347.x.Peer-Reviewed Original ResearchConceptsCytokine expressionLPS treatmentInflammatory compoundsAnti-inflammatory cytokinesIL-8 levelsInterleukin-8 levelsAnti-inflammatory compoundsEnzyme-linked immunosorbent assayCell typesCytokine levelsGlucocorticoid treatmentMaternal bloodDEX treatmentPlacental syncytiumSyncytiotrophoblastImmunosorbent assayLPSPrimary culturesTreatmentCytokinesFourfold increaseExpressionSyncytiaGestationLevels