2006
First-trimester trophoblast cell model gene response to hypoxia
Koklanaris N, Nwachukwu JC, Huang SJ, Guller S, Karpisheva K, Garabedian M, Lee MJ. First-trimester trophoblast cell model gene response to hypoxia. American Journal Of Obstetrics And Gynecology 2006, 194: 687-693. PMID: 16522398, DOI: 10.1016/j.ajog.2006.01.067.Peer-Reviewed Original ResearchConceptsReverse transcriptase-polymerase chain reactionQuantitative reverse transcriptase-polymerase chain reactionTranscriptase-polymerase chain reactionPolymerase chain reactionHypoxia-responsive genesHTR-8/SVneoChain reactionCell linesTrophoblast cell linePregnancy outcomesEarly pregnancyMotility/migrationHypoxic environmentTotal RNAHypoxiaPlacentationMicroarray studiesGene responsesPreeclampsiaPregnancy
2003
Glucocorticoid Enhances Transforming Growth Factor-β Effects on Extracellular Matrix Protein Expression in Human Placental Mesenchymal Cells1
Lee MJ, Ma Y, LaChapelle L, Kadner SS, Guller S. Glucocorticoid Enhances Transforming Growth Factor-β Effects on Extracellular Matrix Protein Expression in Human Placental Mesenchymal Cells1. Biology Of Reproduction 2003, 70: 1246-1252. PMID: 14681196, DOI: 10.1095/biolreprod.103.021956.Peer-Reviewed Original ResearchConceptsPlacental mesenchymal cellsIntrauterine growth restrictionFFN levelsProtein expressionCol IV expressionInteraction of glucocorticoidsDose-dependent mannerHuman term placentaFibronectin protein levelsExtracellular matrix protein expressionUncomplicated pregnanciesPolymerase chain reaction analysisReal-time polymerase chain reaction analysisGrowth restrictionDEX treatmentChain reaction analysisECM proteinsPlacental villiTerm placentaECM protein expressionCol IIIPlacental architectureMatrix protein expressionDexamethasoneGlucocorticoids