2019
Differential gene expression during placentation in pregnancies conceived with different fertility treatments compared with spontaneous pregnancies
Lee B, Koeppel AF, Wang ET, Gonzalez TL, Sun T, Kroener L, Lin Y, Joshi NV, Ghadiali T, Turner SD, Rich SS, Farber CR, Rotter JI, Ida Chen YD, Goodarzi MO, Guller S, Harwood B, Serna TB, Williams J, Pisarska MD. Differential gene expression during placentation in pregnancies conceived with different fertility treatments compared with spontaneous pregnancies. Fertility And Sterility 2019, 111: 535-546. PMID: 30611556, PMCID: PMC7156023, DOI: 10.1016/j.fertnstert.2018.11.005.Peer-Reviewed Original ResearchConceptsFirst trimester placentaSpontaneous pregnancyIVF groupFertility treatmentMaternal medical conditionsTreatment-related factorsAcademic medical centerTranscriptomic profilesGestational age 11Fetal demographicsInfertility groupPregnancy complicationsCohort studySpontaneous conceptionMedical CenterMAIN OUTCOMEMedical conditionsPregnancyDifferent fertility treatmentsIVFLarger studyDifferential gene expressionChorionic villiGene expressionPlacenta
2016
H19 long noncoding RNA alters trophoblast cell migration and invasion by regulating TβR3 in placentae with fetal growth restriction
Zuckerwise L, Li J, Lu L, Men Y, Geng T, Buhimschi CS, Buhimschi IA, Bukowski R, Guller S, Paidas M, Huang Y. H19 long noncoding RNA alters trophoblast cell migration and invasion by regulating TβR3 in placentae with fetal growth restriction. Oncotarget 2016, 7: 38398-38407. PMID: 27223264, PMCID: PMC5122399, DOI: 10.18632/oncotarget.9534.Peer-Reviewed Original ResearchConceptsFetal growth restrictionGrowth restrictionExtravillous trophoblastsDownregulation of H19Type III TGF-β receptorCell migrationEVT cell migrationNon-canonical TGFTGF-β receptorFGR placentasNeurodevelopmental impairmentPerinatal mortalityRisk factorsTherapeutic modalitiesEVT cellsH19 knockdownOnset disordersPlacentaInvasionMolecular mechanismsH19Let-7Novel regulatory targetMorbidityRegulatory pathways
2014
Syncytiotrophoblast Vesicles Show Altered micro-RNA and Haemoglobin Content after Ex-vivo Perfusion of Placentas with Haemoglobin to Mimic Preeclampsia
Cronqvist T, Saljé K, Familari M, Guller S, Schneider H, Gardiner C, Sargent IL, Redman CW, Mörgelin M, Åkerström B, Gram M, Hansson SR. Syncytiotrophoblast Vesicles Show Altered micro-RNA and Haemoglobin Content after Ex-vivo Perfusion of Placentas with Haemoglobin to Mimic Preeclampsia. PLOS ONE 2014, 9: e90020. PMID: 24587192, PMCID: PMC3937405, DOI: 10.1371/journal.pone.0090020.Peer-Reviewed Original ResearchConceptsPathology of preeclampsiaCell-free HbMiR-517bMiR-517aSyncytiotrophoblast vesiclesExtracellular free hemoglobinPerfusion of placentaEx vivo perfusion systemMicro-RNAsMaternal circulationMimic preeclampsiaMaternal sidePerfusion mediumFree hemoglobinHuman placentaPerfusion phaseSurface markersPlacentaMiR-141Hemoglobin contentPerfusionPerfusion systemPreeclampsiaHemoglobinQuantitative PCRIn Vivo and In Vitro Evidence for Placental DNA Damage in Preeclampsia
Tadesse S, Kidane D, Guller S, Luo T, Norwitz NG, Arcuri F, Toti P, Norwitz ER. In Vivo and In Vitro Evidence for Placental DNA Damage in Preeclampsia. PLOS ONE 2014, 9: e86791. PMID: 24466242, PMCID: PMC3899334, DOI: 10.1371/journal.pone.0086791.Peer-Reviewed Original ResearchConceptsDecidual stromal cellsCytotrophoblast cellsMaternal decidual stromal cellsReactive oxygen speciesPlacental oxidative stressFetal-maternal interfaceDNA damagePE placental tissuesAnti-oxidant defensesMultisystem diseasePregnant womenPE placentasFetal sideMaternal deciduaOxidative DNA damagePreeclampsiaPlacental tissueNormal placentaStromal cellsPlacentaOxidative stressVitro EvidenceRepair responseDNA damage/repair responseΓH2AX foci
2013
Decreased Levels of Folate Receptor‐β and Reduced Numbers of Fetal Macrophages (Hofbauer Cells) in Placentas from Pregnancies with Severe Pre‐Eclampsia
Tang Z, Buhimschi IA, Buhimschi CS, Tadesse S, Norwitz E, Niven‐Fairchild T, Huang S, Guller S. Decreased Levels of Folate Receptor‐β and Reduced Numbers of Fetal Macrophages (Hofbauer Cells) in Placentas from Pregnancies with Severe Pre‐Eclampsia. American Journal Of Reproductive Immunology 2013, 70: 104-115. PMID: 23480364, PMCID: PMC3686834, DOI: 10.1111/aji.12112.Peer-Reviewed Original ResearchConceptsSpontaneous preterm birthPre-eclampsiaAge-matched normotensive controlsPreterm pre-eclampsiaSevere pre eclampsiaPathophysiology of PEPregnancy complicationsPreterm birthNormotensive controlsHofbauer cellsFetal mortalityUnknown etiologyPlacental expressionSPTB groupMacrophage functionPlacentaSevere preFetal macrophagesWestern blottingMajor causeProtein expressionPlacental genesReverse transcription-PCR analysisMarker mRNAsTranscription-PCR analysis
2011
Isolation of Hofbauer Cells from Human Term Placentas with High Yield and Purity
Tang Z, Tadesse S, Norwitz E, Mor G, Abrahams VM, Guller S. Isolation of Hofbauer Cells from Human Term Placentas with High Yield and Purity. American Journal Of Reproductive Immunology 2011, 66: 336-348. PMID: 21545365, PMCID: PMC3154981, DOI: 10.1111/j.1600-0897.2011.01006.x.Peer-Reviewed Original ResearchConceptsHofbauer cellsHuman term placentaTerm placentaComplications of pregnancyCell typesMacrophage functionPhagocytotic activityFlow cytometryVillus functionConsensus methodologyVacuolated appearancePhagocytosis assaysPlacentaPercoll gradientCTFuture studiesFibroblastsHBCCellsDaysHigh levelsComplicationsCulture mediumPregnancyMacrophagesPlacental Hofbauer cells and complications of pregnancy
Tang Z, Abrahams VM, Mor G, Guller S. Placental Hofbauer cells and complications of pregnancy. Annals Of The New York Academy Of Sciences 2011, 1221: 103-108. PMID: 21401637, PMCID: PMC3707113, DOI: 10.1111/j.1749-6632.2010.05932.x.Peer-Reviewed Original ResearchConceptsComplications of pregnancyHofbauer cellsHistological chorioamnionitisCytokine-mediated inflammationPlacental Hofbauer cellsPlacental pathophysiologyPlacental macrophagesUnknown etiologyPlacental functionMajor cell typesVillus functionComplicationsPregnancyCell typesSpecific roleGene expressionChorioamnionitisVillitisCellsInflammationGestationPathophysiologyEtiologyPlacentaMacrophages