2001
Tsetse immune responses and trypanosome transmission: Implications for the development of tsetse-based strategies to reduce trypanosomiasis
Hao Z, Kasumba I, Lehane M, Gibson W, Kwon J, Aksoy S. Tsetse immune responses and trypanosome transmission: Implications for the development of tsetse-based strategies to reduce trypanosomiasis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 12648-12653. PMID: 11592981, PMCID: PMC60108, DOI: 10.1073/pnas.221363798.Peer-Reviewed Original ResearchConceptsFat bodyInsect immune systemAntimicrobial peptide genesFat body tissueImmune responsive genesDifferent life stagesTransgenic approachesResponsive genesAntimicrobial genesImmune responseSubtractive cloningPeptide genesAfrican trypanosomesMolecular signalsImmune systemDifferential regulationInfection processLife stagesTrypanosome transmissionParasite transmissionGenesImportant vectorEscherichia coliBiochemical natureDiptericinA novel application of gene arrays: Escherichia coli array provides insight into the biology of the obligate endosymbiont of tsetse flies
Akman L, Aksoy S. A novel application of gene arrays: Escherichia coli array provides insight into the biology of the obligate endosymbiont of tsetse flies. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 7546-7551. PMID: 11404467, PMCID: PMC34705, DOI: 10.1073/pnas.131057498.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsDNA MethylationDNA ReplicationDNA, BacterialDNA, ComplementaryEnterobacteriaceaeEscherichia coliGenome, BacterialMolecular Sequence DataNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisProtein BiosynthesisSymbiosisTranscription, GeneticTsetse FliesConceptsObligate endosymbiontsComplete genome sequence dataGene arrayBiosynthesis of cofactorsE. coli genesGenome sequence dataTsetse fliesFacultative anaerobic organismsObligate symbiontsOrthologous genesWigglesworthia glossinidiaSmall genomesGenome sizeSymbiotic associationGenome informationDNA replicationRelated organismsColi geneSymbiotic lifeSequence dataWigglesworthiaGene productsExpression analysisTransport proteinsExpression profilesMicrosatellite Polymorphism in Tsetse Flies (Diptera: Glossinidae)
Luna C, Bonizzoni M, Cheng Q, Robinson A, Aksoy S, Zheng L. Microsatellite Polymorphism in Tsetse Flies (Diptera: Glossinidae). Journal Of Medical Entomology 2001, 38: 376-381. PMID: 11372961, DOI: 10.1603/0022-2585-38.3.376.Peer-Reviewed Original ResearchConceptsTsetse fliesCertain wild populationsPolymorphic microsatellite lociVectors of trypanosomesG. austeni NewsteadWild populationsMicrosatellite lociIntraspecific variationGenetic variabilityMicrosatellite markersField populationsSubpopulation structureVectorial capacityLociFliesMicrosatellite polymorphismCausative agentMolecular characterization of two serine proteases expressed in gut tissue of the African trypanosome vector, Glossina morsitans morsitans
Yan J, Cheng Q, Li C, Aksoy S. Molecular characterization of two serine proteases expressed in gut tissue of the African trypanosome vector, Glossina morsitans morsitans. Insect Molecular Biology 2001, 10: 47-56. PMID: 11240636, DOI: 10.1046/j.1365-2583.2001.00232.x.Peer-Reviewed Original ResearchConceptsSerine proteasesSerine protease-encoding genePutative mature proteinHydrophobic signal peptide sequenceInvertebrate serine proteasesProtease-encoding genesPost-transcriptional levelAmino acid mature peptideGut tissueSer catalytic triadSignal peptide sequenceRegulation of expressionPattern of expressionSerine protease 1Insect gutMature proteinVector insectsDeduced peptideChymotrypsin-like proteaseTsetse genomeSpecific residuesCatalytic triadAfrican trypanosomesMature peptidePathogen establishment
2000
A family of genes with growth factor and adenosine deaminase similarity are preferentially expressed in the salivary glands of Glossina m. morsitans
Li S, Aksoy S. A family of genes with growth factor and adenosine deaminase similarity are preferentially expressed in the salivary glands of Glossina m. morsitans. Gene 2000, 252: 83-93. PMID: 10903440, DOI: 10.1016/s0378-1119(00)00226-2.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine DeaminaseAmino Acid SequenceAnimalsBase SequenceBlotting, NorthernBlotting, SouthernDNADNA, ComplementaryFemaleGene Expression RegulationGene Expression Regulation, DevelopmentalGrowth SubstancesMaleMolecular Sequence DataRNA, MessengerSalivaSalivary GlandsSequence Analysis, DNASpecies SpecificityTissue DistributionTsetse FliesConceptsOpen reading frameFemale adult fliesDeduced protein sequenceSignal peptide characteristicDifferent fly tissuesFamily of genesFull-length cDNAAmino acid identityLarval developmental stagesRegulation of expressionN-terminal regionGrowth factorAmino acid residuesTissues of adultAnalysis of proteinsGenomic arrangementPutative proteinFly tissuesSarcophaga peregrinaAcid identityHydrophobic sequenceCDNA libraryReading frameAdult fliesProtein sequences
1998
Tsetse thrombin inhibitor: Bloodmeal-induced expression of an anticoagulant in salivary glands and gut tissue of Glossina morsitans morsitans
Cappello M, Li S, Chen X, Li C, Harrison L, Narashimhan S, Beard C, Aksoy S. Tsetse thrombin inhibitor: Bloodmeal-induced expression of an anticoagulant in salivary glands and gut tissue of Glossina morsitans morsitans. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 14290-14295. PMID: 9826693, PMCID: PMC24366, DOI: 10.1073/pnas.95.24.14290.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAnticoagulantsAntithrombin ProteinsAntithrombinsBase SequenceBloodCloning, MolecularDigestive SystemDNA, ComplementaryEatingEscherichia coliFemaleGene Expression RegulationInsect ProteinsMaleMolecular Sequence DataPupaRecombinant ProteinsRNA, MessengerSalivary GlandsThrombinTranscription, GeneticTsetse FliesConceptsTsetse thrombin inhibitorPutative secretory signal peptideNative proteinGlossina morsitans morsitansFull-length cDNADegenerate oligonucleotide probeSecretory signal peptideSalivary gland cDNA libraryLack of homologyMorsitans morsitansUntranslated segmentSignal peptideInhibitor geneCDNA librarySingle copyTsetse genomeAdult tsetse fliesFamily of moleculesSegment codesEscherichia coliOptimal inhibitory activityOligonucleotide probesTsetse fliesSalivary glandsGut tissue