2005
Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host
Toh H, Weiss BL, Perkin SA, Yamashita A, Oshima K, Hattori M, Aksoy S. Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host. Genome Research 2005, 16: 149-156. PMID: 16365377, PMCID: PMC1361709, DOI: 10.1101/gr.4106106.Peer-Reviewed Original ResearchConceptsHost developmental stageTemporal expression profilesDifferent temporal expression profilesGenome erosionMutualistic lifestylesGene inventorySymbiont infectionSymbiotic lifestyleHost developmentTranscript abundanceMetabolism of carbohydratesFlagellum structureSodalisComplete sequenceExpression profilesSPI-2SPI-1ChromosomesTsetse hostBacterial speciesVeterinary significancePathogenic microbesDevelopmental stagesPseudogenesTsetse flies
2001
Genome Size Determination and Coding Capacity of Sodalis glossinidius, an Enteric Symbiont of Tsetse Flies, as Revealed by Hybridization to Escherichia coliGene Arrays
Akman L, Rio R, Beard C, Aksoy S. Genome Size Determination and Coding Capacity of Sodalis glossinidius, an Enteric Symbiont of Tsetse Flies, as Revealed by Hybridization to Escherichia coliGene Arrays. Journal Of Bacteriology 2001, 183: 4517-4525. PMID: 11443086, PMCID: PMC95346, DOI: 10.1128/jb.183.15.4517-4525.2001.Peer-Reviewed Original ResearchConceptsGenome sizeLong evolutionary timeGenome size determinationSecondary symbiont SodalisAT-rich genomeConserved gene sequencesWhole genome sequencesTsetse fliesEnteric symbiontsObligate symbiontsOrthologous genesRecent molecular characterizationComparative genomicsMicrobial genomesSymbiotic microorganismsEvolutionary timeTsetse fecundityDNA replicationGenome sequenceGenomic aspectsGenomic contentGene sequencesMolecular basisRelated bacteriaExtrachromosomal plasmidsMicrosatellite Polymorphism in Tsetse Flies (Diptera: Glossinidae)
Luna C, Bonizzoni M, Cheng Q, Robinson A, Aksoy S, Zheng L. Microsatellite Polymorphism in Tsetse Flies (Diptera: Glossinidae). Journal Of Medical Entomology 2001, 38: 376-381. PMID: 11372961, DOI: 10.1603/0022-2585-38.3.376.Peer-Reviewed Original ResearchConceptsTsetse fliesCertain wild populationsPolymorphic microsatellite lociVectors of trypanosomesG. austeni NewsteadWild populationsMicrosatellite lociIntraspecific variationGenetic variabilityMicrosatellite markersField populationsSubpopulation structureVectorial capacityLociFliesMicrosatellite polymorphismCausative agentMolecular characterization of two serine proteases expressed in gut tissue of the African trypanosome vector, Glossina morsitans morsitans
Yan J, Cheng Q, Li C, Aksoy S. Molecular characterization of two serine proteases expressed in gut tissue of the African trypanosome vector, Glossina morsitans morsitans. Insect Molecular Biology 2001, 10: 47-56. PMID: 11240636, DOI: 10.1046/j.1365-2583.2001.00232.x.Peer-Reviewed Original ResearchConceptsSerine proteasesSerine protease-encoding genePutative mature proteinHydrophobic signal peptide sequenceInvertebrate serine proteasesProtease-encoding genesPost-transcriptional levelAmino acid mature peptideGut tissueSer catalytic triadSignal peptide sequenceRegulation of expressionPattern of expressionSerine protease 1Insect gutMature proteinVector insectsDeduced peptideChymotrypsin-like proteaseTsetse genomeSpecific residuesCatalytic triadAfrican trypanosomesMature peptidePathogen establishment
2000
A family of genes with growth factor and adenosine deaminase similarity are preferentially expressed in the salivary glands of Glossina m. morsitans
Li S, Aksoy S. A family of genes with growth factor and adenosine deaminase similarity are preferentially expressed in the salivary glands of Glossina m. morsitans. Gene 2000, 252: 83-93. PMID: 10903440, DOI: 10.1016/s0378-1119(00)00226-2.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine DeaminaseAmino Acid SequenceAnimalsBase SequenceBlotting, NorthernBlotting, SouthernDNADNA, ComplementaryFemaleGene Expression RegulationGene Expression Regulation, DevelopmentalGrowth SubstancesMaleMolecular Sequence DataRNA, MessengerSalivaSalivary GlandsSequence Analysis, DNASpecies SpecificityTissue DistributionTsetse FliesConceptsOpen reading frameFemale adult fliesDeduced protein sequenceSignal peptide characteristicDifferent fly tissuesFamily of genesFull-length cDNAAmino acid identityLarval developmental stagesRegulation of expressionN-terminal regionGrowth factorAmino acid residuesTissues of adultAnalysis of proteinsGenomic arrangementPutative proteinFly tissuesSarcophaga peregrinaAcid identityHydrophobic sequenceCDNA libraryReading frameAdult fliesProtein sequences
1999
Concordant Evolution of a Symbiont with Its Host Insect Species: Molecular Phylogeny of Genus Glossina and Its Bacteriome-Associated Endosymbiont, Wigglesworthia glossinidia
Chen X, Li S, Aksoy S. Concordant Evolution of a Symbiont with Its Host Insect Species: Molecular Phylogeny of Genus Glossina and Its Bacteriome-Associated Endosymbiont, Wigglesworthia glossinidia. Journal Of Molecular Evolution 1999, 48: 49-58. PMID: 9873076, DOI: 10.1007/pl00006444.Peer-Reviewed Original ResearchConceptsHost insect speciesRDNA sequence analysisInsect speciesGroup speciesSequence analysisSister-group relationshipSpacer 2 (ITS2) regionParasitic African trypanosomesWigglesworthia glossinidiaMolecular phylogenyDifferentiated epithelial cellsGlossina austeniPrimary symbiontSecondary symbiontsSymbiotic associationDistinct lineagesRibosomal DNAGenus WolbachiaSymbiotic organismsTaxonomic placementΓ-subdivisionITS-2Third organismMidgut cellsAfrican trypanosomes
1998
Tsetse thrombin inhibitor: Bloodmeal-induced expression of an anticoagulant in salivary glands and gut tissue of Glossina morsitans morsitans
Cappello M, Li S, Chen X, Li C, Harrison L, Narashimhan S, Beard C, Aksoy S. Tsetse thrombin inhibitor: Bloodmeal-induced expression of an anticoagulant in salivary glands and gut tissue of Glossina morsitans morsitans. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 14290-14295. PMID: 9826693, PMCID: PMC24366, DOI: 10.1073/pnas.95.24.14290.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAnticoagulantsAntithrombin ProteinsAntithrombinsBase SequenceBloodCloning, MolecularDigestive SystemDNA, ComplementaryEatingEscherichia coliFemaleGene Expression RegulationInsect ProteinsMaleMolecular Sequence DataPupaRecombinant ProteinsRNA, MessengerSalivary GlandsThrombinTranscription, GeneticTsetse FliesConceptsTsetse thrombin inhibitorPutative secretory signal peptideNative proteinGlossina morsitans morsitansFull-length cDNADegenerate oligonucleotide probeSecretory signal peptideSalivary gland cDNA libraryLack of homologyMorsitans morsitansUntranslated segmentSignal peptideInhibitor geneCDNA librarySingle copyTsetse genomeAdult tsetse fliesFamily of moleculesSegment codesEscherichia coliOptimal inhibitory activityOligonucleotide probesTsetse fliesSalivary glandsGut tissue
1993
Phylogenetically distant symbiotic microorganisms reside in Glossina midgut and ovary tissues
O'NEILL S, GOODING R, AKSOY S. Phylogenetically distant symbiotic microorganisms reside in Glossina midgut and ovary tissues. Medical And Veterinary Entomology 1993, 7: 377-383. PMID: 8268495, DOI: 10.1111/j.1365-2915.1993.tb00709.x.Peer-Reviewed Original ResearchConceptsIntracellular bacterial symbiontsBlood-feeding insectsDNA oligonucleotide primersBacterial symbiontsSymbiotic microorganismsAlpha subdivisionSymbiotic bacteriaGamma subdivisionGenus WolbachiaGlossina speciesPolymerase chain reactionOvary tissuesOligonucleotide primersTsetse fliesMidgutSpeciesSymbiontsInsectsWolbachiaProteobacteriaSubspeciesChain reactionFliesMicroorganismsTissue
1992
Spliced leader RNA sequences of Trypanosoma rangeli are organized within the 5S rRNA-encoding genes
Aksoy S, Shay G, Villaneuva M, Beard C, Richards F. Spliced leader RNA sequences of Trypanosoma rangeli are organized within the 5S rRNA-encoding genes. Gene 1992, 113: 239-243. PMID: 1572544, DOI: 10.1016/0378-1119(92)90401-a.Peer-Reviewed Original ResearchSpliced leader RNA and 5S rRNA genes in Herpetomonas spp. are genetically linked
Aksoy S. Spliced leader RNA and 5S rRNA genes in Herpetomonas spp. are genetically linked. Nucleic Acids Research 1992, 20: 913-913. PMID: 1542583, PMCID: PMC312040, DOI: 10.1093/nar/20.4.913.Peer-Reviewed Original Research
1990
SLACS retrotransposon from Trypanosoma brucei gambiense is similar to mammalian LINEs
Aksoy S, Williams S, Chang S, Richards F. SLACS retrotransposon from Trypanosoma brucei gambiense is similar to mammalian LINEs. Nucleic Acids Research 1990, 18: 785-792. PMID: 2156231, PMCID: PMC330328, DOI: 10.1093/nar/18.4.785.Peer-Reviewed Original Research