2023
Genomic evidence of sex chromosome aneuploidy and infection-associated genotypes in the tsetse fly Glossina fuscipes, the major vector of African trypanosomiasis in Uganda
Saarman N, Son J, Zhao H, Cosme L, Kong Y, Li M, Wang S, Weiss B, Echodu R, Opiro R, Aksoy S, Caccone A. Genomic evidence of sex chromosome aneuploidy and infection-associated genotypes in the tsetse fly Glossina fuscipes, the major vector of African trypanosomiasis in Uganda. Infection Genetics And Evolution 2023, 114: 105501. PMID: 37709241, PMCID: PMC10593118, DOI: 10.1016/j.meegid.2023.105501.Peer-Reviewed Original ResearchMeSH KeywordsAneuploidyAnimalsGenome-Wide Association StudyGenomicsGenotypeHumansMammalsSex ChromosomesTrypanosomaTrypanosomiasis, AfricanTsetse FliesUgandaConceptsGenome-wide association analysisNovel vector control strategiesAnimal African trypanosomiasisSex chromosome aneuploidyNatural populationsGenome assemblySex chromosomesGenomic regionsGenomic evidenceGenetic variationBp upstreamAutosomal SNPsSeq dataTrypanosome infectionTrypanosome parasitesAssociation analysisMolecular pathwaysAssembly metricsLinkage disequilibriumLecithin-cholesterol acyltransferaseAfrican trypanosomiasisMajor vectorGenomeSNPsPrimary vector
2020
Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens
Vigneron A, O'Neill MB, Weiss BL, Savage AF, Campbell OC, Kamhawi S, Valenzuela JG, Aksoy S. Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 2613-2621. PMID: 31964820, PMCID: PMC7007551, DOI: 10.1073/pnas.1914423117.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingRNA sequencingInfectious metacyclic formsMetacyclic parasitesMammalian host environmentFly salivary glandsMajor cell clustersClustering of cellsTsetse salivary glandsFamily proteinsDevelopmental programMammalian hostsMetacyclic cellsProtein transcriptsTrypanosoma bruceiDevelopmental processesGene expressionAfrican trypanosomesExpression profilesMolecular mechanismsSalivary glandsNew hostSurface localizationTrypanosome transmissionMetacyclogenesis
2019
Colonization of the tsetse fly midgut with commensal Kosakonia cowanii Zambiae inhibits trypanosome infection establishment
Weiss BL, Maltz MA, Vigneron A, Wu Y, Walter KS, O’Neill M, Wang J, Aksoy S. Colonization of the tsetse fly midgut with commensal Kosakonia cowanii Zambiae inhibits trypanosome infection establishment. PLOS Pathogens 2019, 15: e1007470. PMID: 30817773, PMCID: PMC6394900, DOI: 10.1371/journal.ppat.1007470.Peer-Reviewed Original ResearchConceptsRefractory phenotypeEnormous socio-economic burdenWild-type counterpartsInfection establishmentSocio-economic burdenMidgut environmentEntomopathogenic Serratia marcescensEndemic regionsPathogenic trypanosomesInfectionStable infectionAdverse effectsAnimal African trypanosomiasesVector competenceGutCurrent disease control strategiesSaharan AfricaDisease control strategiesSurvivalSerratia marcescensTsetse gutExogenous bacteriumFly survivalNovel strategyPhenotype
2017
Human African trypanosomiasis control: Achievements and challenges
Aksoy S, Buscher P, Lehane M, Solano P, Van Den Abbeele J. Human African trypanosomiasis control: Achievements and challenges. PLOS Neglected Tropical Diseases 2017, 11: e0005454. PMID: 28426685, PMCID: PMC5398477, DOI: 10.1371/journal.pntd.0005454.Peer-Reviewed Original Research
2016
Mammalian African trypanosome VSG coat enhances tsetse’s vector competence
Aksoy E, Vigneron A, Bing X, Zhao X, O'Neill M, Wu YN, Bangs JD, Weiss BL, Aksoy S. Mammalian African trypanosome VSG coat enhances tsetse’s vector competence. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: 6961-6966. PMID: 27185908, PMCID: PMC4922192, DOI: 10.1073/pnas.1600304113.Peer-Reviewed Original ResearchConceptsVariant surface glycoproteinPeritrophic matrixMammalian hostsVector competenceTranscription factor familyMidgut homeostasisTsetse midgutTrypanosome biologyFactor familyPM barrierCoat proteinNovel functionAfrican trypanosomesTsetse vectorInfection processParasite developmentAnimal trypanosomiasesAntigenic variationVSG moleculesVSG coatBiological vectorsMidgutProtozoan parasiteDisease transmissionTsetse flies
2014
Genome Sequence of the Tsetse Fly (Glossina morsitans): Vector of African Trypanosomiasis
Initiative I, Attardo G, Abila P, Auma J, Baumann A, Benoit J, Brelsfoard C, Ribeiro J, Cotton J, Pham D, Darby A, Van Den Abbeele J, Denlinger D, Field L, Nyanjom S, Gaunt M, Geiser D, Gomulski L, Haines L, Hansen I, Jones J, Kibet C, Kinyua J, Larkin D, Lehane M, Rio R, Macdonald S, Macharia R, Malacrida A, Marco H, Marucha K, Masiga D, Meuti M, Mireji P, Obiero G, Koekemoer J, Okoro C, Omedo I, Osamor V, Balyeidhusa A, Peyton J, Price D, Quail M, Ramphul U, Rawlings N, Riehle M, Robertson H, Sanders M, Scott M, Dashti Z, Snyder A, Srivastava T, Stanley E, Swain M, Hughes D, Tarone A, Taylor T, Telleria E, Thomas G, Walshe D, Wilson R, Winzerling J, Acosta-Serrano A, Aksoy S, Arensburger P, Aslett M, Bateta R, Benkahla A, Berriman M, Bourtzis K, Caers J, Caljon G, Christoffels A, Falchetto M, Friedrich M, Fu S, Gäde G, Githinji G, Gregory R, Hall N, Harkins G, Hattori M, Hertz-Fowler C, Hide W, Hu W, Imanishi T, Inoue N, Jonas M, Kawahara Y, Koffi M, Kruger A, Lawson D, Lehane S, Lehväslaiho H, Luiz T, Makgamathe M, Malele I, Manangwa O, Manga L, Megy K, Michalkova V, Mpondo F, Mramba F, Msangi A, Mulder N, Murilla G, Mwangi S, Okedi L, Ommeh S, Ooi C, Ouma J, Panji S, Ravel S, Rose C, Sakate R, Schoofs L, Scolari F, Sharma V, Sim C, Siwo G, Solano P, Stephens D, Suzuki Y, Sze S, Touré Y, Toyoda A, Tsiamis G, Tu Z, Wamalwa M, Wamwiri F, Wang J, Warren W, Watanabe J, Weiss B, Willis J, Wincker P, Zhang Q, Zhou J. Genome Sequence of the Tsetse Fly (Glossina morsitans): Vector of African Trypanosomiasis. Science 2014, 344: 380-386. PMID: 24763584, PMCID: PMC4077534, DOI: 10.1126/science.1249656.Peer-Reviewed Original ResearchConceptsGenome sequenceLactation-specific proteinsProtein-encoding genesBacterial genome sequencesPathogen recognition proteinsTsetse fliesMicrobial symbiosesTsetse biologyViviparous reproductionGenome dataRecognition proteinsSole vectorsChromosomal integrationDisease vectorsAfrican trypanosomiasisGenomeGenesFliesProteinSequenceMultiple aspectsHuman African trypanosomiasisImportant insightsMultiple discoveriesSymbioses
2001
Molecular characterization of two serine proteases expressed in gut tissue of the African trypanosome vector, Glossina morsitans morsitans
Yan J, Cheng Q, Li C, Aksoy S. Molecular characterization of two serine proteases expressed in gut tissue of the African trypanosome vector, Glossina morsitans morsitans. Insect Molecular Biology 2001, 10: 47-56. PMID: 11240636, DOI: 10.1046/j.1365-2583.2001.00232.x.Peer-Reviewed Original ResearchConceptsSerine proteasesSerine protease-encoding genePutative mature proteinHydrophobic signal peptide sequenceInvertebrate serine proteasesProtease-encoding genesPost-transcriptional levelAmino acid mature peptideGut tissueSer catalytic triadSignal peptide sequenceRegulation of expressionPattern of expressionSerine protease 1Insect gutMature proteinVector insectsDeduced peptideChymotrypsin-like proteaseTsetse genomeSpecific residuesCatalytic triadAfrican trypanosomesMature peptidePathogen establishmentProspects for control of African trypanosomiasis by tsetse vector manipulation
Aksoy S, O'Neill S, Maudlin I, Dale C, Robinson A. Prospects for control of African trypanosomiasis by tsetse vector manipulation. Trends In Parasitology 2001, 17: 29-35. PMID: 11137738, DOI: 10.1016/s1471-4922(00)01850-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedHumansInsect VectorsMaleTrypanosomiasis, AfricanTsetse FliesConceptsArea-wide approachHuman disease managementLivestock ownersAnimal diseasesAgricultural outputMolecular genetic approachesImportant insectsInfected hostDisease managementEconomic incentivesTsetse populationsVector manipulationGenetic approachesMammalian hostsVector competenceEffective vaccineDiseases reliesHostTrypanosomiasisControl strategyInsectsAfrican trypanosomiasisTsetseLong runControl