2022
Microbe Profile: Wigglesworthia glossinidia: the tsetse fly’s significant other
Weiss BL, Rio RVM, Aksoy S. Microbe Profile: Wigglesworthia glossinidia: the tsetse fly’s significant other. Microbiology 2022, 168: 001242. PMID: 36129743, PMCID: PMC10723186, DOI: 10.1099/mic.0.001242.Peer-Reviewed Original ResearchConceptsPhysiological homeostasisNutritional roleEssential nutritional roleUnique physiological adaptationsTsetse fliesFly microbiotaWigglesworthia glossinidiaObligate mutualistsHost fitnessAncient associationParasitic trypanosomesLarval periodPhysiological adaptationsFitness outcomesTsetse's abilityAntimicrobial responsesImmune systemAmidasesFliesMicrobiotaMutualistsWigglesworthiaEndosymbiontsGenomeB vitamins
2021
Infection with endosymbiotic Spiroplasma disrupts tsetse (Glossina fuscipes fuscipes) metabolic and reproductive homeostasis
Son JH, Weiss BL, Schneider DI, Dera KM, Gstöttenmayer F, Opiro R, Echodu R, Saarman NP, Attardo GM, Onyango M, Abdalla A, Aksoy S. Infection with endosymbiotic Spiroplasma disrupts tsetse (Glossina fuscipes fuscipes) metabolic and reproductive homeostasis. PLOS Pathogens 2021, 17: e1009539. PMID: 34529715, PMCID: PMC8478229, DOI: 10.1371/journal.ppat.1009539.Peer-Reviewed Original ResearchConceptsReproductive fitnessSpiroplasma infectionSex-biased gene expressionHigh-throughput RNA sequencingReproductive physiologyIntrauterine larval developmentMale reproductive fitnessPathogenic African trypanosomesEndosymbiotic bacteriaFly resistanceTsetse fecundityFemale spermathecaFemale fecundityRNA sequencingLarval developmentSpiroplasmaGene expressionAfrican trypanosomesReproductive tissuesReproductive homeostasisTsetse hostHuman diseasesPopulation sizeProtective phenotypeLab lines
2019
Colonization of the tsetse fly midgut with commensal Kosakonia cowanii Zambiae inhibits trypanosome infection establishment
Weiss BL, Maltz MA, Vigneron A, Wu Y, Walter KS, O’Neill M, Wang J, Aksoy S. Colonization of the tsetse fly midgut with commensal Kosakonia cowanii Zambiae inhibits trypanosome infection establishment. PLOS Pathogens 2019, 15: e1007470. PMID: 30817773, PMCID: PMC6394900, DOI: 10.1371/journal.ppat.1007470.Peer-Reviewed Original ResearchConceptsRefractory phenotypeEnormous socio-economic burdenWild-type counterpartsInfection establishmentSocio-economic burdenMidgut environmentEntomopathogenic Serratia marcescensEndemic regionsPathogenic trypanosomesInfectionStable infectionAdverse effectsAnimal African trypanosomiasesVector competenceGutCurrent disease control strategiesSaharan AfricaDisease control strategiesSurvivalSerratia marcescensTsetse gutExogenous bacteriumFly survivalNovel strategyPhenotype
2017
Unravelling the relationship between the tsetse fly and its obligate symbiont Wigglesworthia: transcriptomic and metabolomic landscapes reveal highly integrated physiological networks
Bing X, Attardo GM, Vigneron A, Aksoy E, Scolari F, Malacrida A, Weiss BL, Aksoy S. Unravelling the relationship between the tsetse fly and its obligate symbiont Wigglesworthia: transcriptomic and metabolomic landscapes reveal highly integrated physiological networks. Proceedings Of The Royal Society B 2017, 284: 20170360. PMID: 28659447, PMCID: PMC5489720, DOI: 10.1098/rspb.2017.0360.Peer-Reviewed Original ResearchConceptsPeptidoglycan recognition proteinsAfrican trypanosome parasitesTsetse fliesAmino acid metabolismWigglesworthia glossinidiaMultiple metabolic pathwaysObligate endosymbiontsMolecular chaperonesTransport machineryVertebrate bloodMetabolomic landscapeRecognition proteinsSeq analysisSymbiotic dialogueNucleotide biosynthesisAdenosyl methionineBiological functionsSpecialized cellsTsetse survivalTrypanosome parasitesEssential cofactorMetabolic pathwaysNew biological targetsAcid metabolismPhysiological networks
2014
Genome Sequence of the Tsetse Fly (Glossina morsitans): Vector of African Trypanosomiasis
Initiative I, Attardo G, Abila P, Auma J, Baumann A, Benoit J, Brelsfoard C, Ribeiro J, Cotton J, Pham D, Darby A, Van Den Abbeele J, Denlinger D, Field L, Nyanjom S, Gaunt M, Geiser D, Gomulski L, Haines L, Hansen I, Jones J, Kibet C, Kinyua J, Larkin D, Lehane M, Rio R, Macdonald S, Macharia R, Malacrida A, Marco H, Marucha K, Masiga D, Meuti M, Mireji P, Obiero G, Koekemoer J, Okoro C, Omedo I, Osamor V, Balyeidhusa A, Peyton J, Price D, Quail M, Ramphul U, Rawlings N, Riehle M, Robertson H, Sanders M, Scott M, Dashti Z, Snyder A, Srivastava T, Stanley E, Swain M, Hughes D, Tarone A, Taylor T, Telleria E, Thomas G, Walshe D, Wilson R, Winzerling J, Acosta-Serrano A, Aksoy S, Arensburger P, Aslett M, Bateta R, Benkahla A, Berriman M, Bourtzis K, Caers J, Caljon G, Christoffels A, Falchetto M, Friedrich M, Fu S, Gäde G, Githinji G, Gregory R, Hall N, Harkins G, Hattori M, Hertz-Fowler C, Hide W, Hu W, Imanishi T, Inoue N, Jonas M, Kawahara Y, Koffi M, Kruger A, Lawson D, Lehane S, Lehväslaiho H, Luiz T, Makgamathe M, Malele I, Manangwa O, Manga L, Megy K, Michalkova V, Mpondo F, Mramba F, Msangi A, Mulder N, Murilla G, Mwangi S, Okedi L, Ommeh S, Ooi C, Ouma J, Panji S, Ravel S, Rose C, Sakate R, Schoofs L, Scolari F, Sharma V, Sim C, Siwo G, Solano P, Stephens D, Suzuki Y, Sze S, Touré Y, Toyoda A, Tsiamis G, Tu Z, Wamalwa M, Wamwiri F, Wang J, Warren W, Watanabe J, Weiss B, Willis J, Wincker P, Zhang Q, Zhou J. Genome Sequence of the Tsetse Fly (Glossina morsitans): Vector of African Trypanosomiasis. Science 2014, 344: 380-386. PMID: 24763584, PMCID: PMC4077534, DOI: 10.1126/science.1249656.Peer-Reviewed Original ResearchConceptsGenome sequenceLactation-specific proteinsProtein-encoding genesBacterial genome sequencesPathogen recognition proteinsTsetse fliesMicrobial symbiosesTsetse biologyViviparous reproductionGenome dataRecognition proteinsSole vectorsChromosomal integrationDisease vectorsAfrican trypanosomiasisGenomeGenesFliesProteinSequenceMultiple aspectsHuman African trypanosomiasisImportant insightsMultiple discoveriesSymbioses
2012
PGRP-LB is a maternally transmitted immune milk protein that influences symbiosis and parasitism in tsetse’s offspring
Wang J, Aksoy S. PGRP-LB is a maternally transmitted immune milk protein that influences symbiosis and parasitism in tsetse’s offspring. Proceedings Of The National Academy Of Sciences Of The United States Of America 2012, 109: 10552-10557. PMID: 22689989, PMCID: PMC3387098, DOI: 10.1073/pnas.1116431109.Peer-Reviewed Original ResearchConceptsMother's milkImmune deficiency (IMD) pathwayPGRP-LBHost immune systemImmune system developmentIntrauterine environmentNutritional supplementationDietary supplementationHyperimmune responseImmune systemImpact immunityNewborn progenyParasite infectionAdultsParasitized adultsPeptidoglycan recognition proteinsSuch adultsImmunitySupplementationAntitrypanosomal activityPivotal roleMilkInductionHost fecundityMilk proteinsObligate Symbionts Activate Immune System Development in the Tsetse Fly
Weiss BL, Maltz M, Aksoy S. Obligate Symbionts Activate Immune System Development in the Tsetse Fly. The Journal Of Immunology 2012, 188: 3395-3403. PMID: 22368278, PMCID: PMC3311772, DOI: 10.4049/jimmunol.1103691.Peer-Reviewed Original ResearchConceptsSymbiotic bacteriaImmune system developmentNovel evolutionary adaptationImmunity-related genesObligate symbiontsSymbiotic microbesObligate mutualistsViviparous modeProper immune system functionEvolutionary adaptationPhagocytic hemocytesMolecular mechanismsCell extractsMolecular componentsSusceptible phenotypeNonpathogenic Escherichia coliEscherichia coliTsetse fliesImmune systemFliesAtypical expressionHemocytesImmune system functionPhenotypeTsetse
2011
Wolbachia Symbiont Infections Induce Strong Cytoplasmic Incompatibility in the Tsetse Fly Glossina morsitans
Alam U, Medlock J, Brelsfoard C, Pais R, Lohs C, Balmand S, Carnogursky J, Heddi A, Takac P, Galvani A, Aksoy S. Wolbachia Symbiont Infections Induce Strong Cytoplasmic Incompatibility in the Tsetse Fly Glossina morsitans. PLOS Pathogens 2011, 7: e1002415. PMID: 22174680, PMCID: PMC3234226, DOI: 10.1371/journal.ppat.1002415.Peer-Reviewed Original ResearchTsetse Immune System Maturation Requires the Presence of Obligate Symbionts in Larvae
Weiss BL, Wang J, Aksoy S. Tsetse Immune System Maturation Requires the Presence of Obligate Symbionts in Larvae. PLOS Biology 2011, 9: e1000619. PMID: 21655301, PMCID: PMC3104962, DOI: 10.1371/journal.pbio.1000619.Peer-Reviewed Original ResearchConceptsIntrauterine larvaeBeneficial microbial symbiontsSpecific host phenotypesMilk gland secretionsHost physiological processesCo-evolutionary adaptationMicrobial symbiontsWigglesworthia glossinidiaObligate mutualistsHost phenotypePhysiological processesImmune system homeostasisWigglesworthiaTsetse fliesImportant functionsGland secretionSystem homeostasisFliesGlossina morsitansMutualistsSymbiontsDietary supplementationLarvaeHomeostasisPhenotype
2009
Interactions between mutualist Wigglesworthia and tsetse peptidoglycan recognition protein (PGRP-LB) influence trypanosome transmission
Wang J, Wu Y, Yang G, Aksoy S. Interactions between mutualist Wigglesworthia and tsetse peptidoglycan recognition protein (PGRP-LB) influence trypanosome transmission. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 12133-12138. PMID: 19587241, PMCID: PMC2715537, DOI: 10.1073/pnas.0901226106.Peer-Reviewed Original ResearchConceptsImmune deficiencyPGRP-LBHost immune responsePeptidoglycan recognition proteinsAnti-protozoal activityTsetse's abilityImmune responseInfection susceptibilityHost immunityExpression correlatesNormal adultsInfectionHost susceptibilityAntimicrobial peptidesParasitized adultsTrypanosome infectionAdult tsetseRecognition proteinsLimited exposureAdultsForeign microbesRNA interferencePathway functionActivationSusceptibility
2005
Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host
Toh H, Weiss BL, Perkin SA, Yamashita A, Oshima K, Hattori M, Aksoy S. Massive genome erosion and functional adaptations provide insights into the symbiotic lifestyle of Sodalis glossinidius in the tsetse host. Genome Research 2005, 16: 149-156. PMID: 16365377, PMCID: PMC1361709, DOI: 10.1101/gr.4106106.Peer-Reviewed Original ResearchConceptsHost developmental stageTemporal expression profilesDifferent temporal expression profilesGenome erosionMutualistic lifestylesGene inventorySymbiont infectionSymbiotic lifestyleHost developmentTranscript abundanceMetabolism of carbohydratesFlagellum structureSodalisComplete sequenceExpression profilesSPI-2SPI-1ChromosomesTsetse hostBacterial speciesVeterinary significancePathogenic microbesDevelopmental stagesPseudogenesTsetse flies
2001
Genome Size Determination and Coding Capacity of Sodalis glossinidius, an Enteric Symbiont of Tsetse Flies, as Revealed by Hybridization to Escherichia coliGene Arrays
Akman L, Rio R, Beard C, Aksoy S. Genome Size Determination and Coding Capacity of Sodalis glossinidius, an Enteric Symbiont of Tsetse Flies, as Revealed by Hybridization to Escherichia coliGene Arrays. Journal Of Bacteriology 2001, 183: 4517-4525. PMID: 11443086, PMCID: PMC95346, DOI: 10.1128/jb.183.15.4517-4525.2001.Peer-Reviewed Original ResearchConceptsGenome sizeLong evolutionary timeGenome size determinationSecondary symbiont SodalisAT-rich genomeConserved gene sequencesWhole genome sequencesTsetse fliesEnteric symbiontsObligate symbiontsOrthologous genesRecent molecular characterizationComparative genomicsMicrobial genomesSymbiotic microorganismsEvolutionary timeTsetse fecundityDNA replicationGenome sequenceGenomic aspectsGenomic contentGene sequencesMolecular basisRelated bacteriaExtrachromosomal plasmidsA novel application of gene arrays: Escherichia coli array provides insight into the biology of the obligate endosymbiont of tsetse flies
Akman L, Aksoy S. A novel application of gene arrays: Escherichia coli array provides insight into the biology of the obligate endosymbiont of tsetse flies. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 7546-7551. PMID: 11404467, PMCID: PMC34705, DOI: 10.1073/pnas.131057498.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBacterial ProteinsDNA MethylationDNA ReplicationDNA, BacterialDNA, ComplementaryEnterobacteriaceaeEscherichia coliGenome, BacterialMolecular Sequence DataNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisProtein BiosynthesisSymbiosisTranscription, GeneticTsetse FliesConceptsObligate endosymbiontsComplete genome sequence dataGene arrayBiosynthesis of cofactorsE. coli genesGenome sequence dataTsetse fliesFacultative anaerobic organismsObligate symbiontsOrthologous genesWigglesworthia glossinidiaSmall genomesGenome sizeSymbiotic associationGenome informationDNA replicationRelated organismsColi geneSymbiotic lifeSequence dataWigglesworthiaGene productsExpression analysisTransport proteinsExpression profiles
1999
Concordant Evolution of a Symbiont with Its Host Insect Species: Molecular Phylogeny of Genus Glossina and Its Bacteriome-Associated Endosymbiont, Wigglesworthia glossinidia
Chen X, Li S, Aksoy S. Concordant Evolution of a Symbiont with Its Host Insect Species: Molecular Phylogeny of Genus Glossina and Its Bacteriome-Associated Endosymbiont, Wigglesworthia glossinidia. Journal Of Molecular Evolution 1999, 48: 49-58. PMID: 9873076, DOI: 10.1007/pl00006444.Peer-Reviewed Original ResearchConceptsHost insect speciesRDNA sequence analysisInsect speciesGroup speciesSequence analysisSister-group relationshipSpacer 2 (ITS2) regionParasitic African trypanosomesWigglesworthia glossinidiaMolecular phylogenyDifferentiated epithelial cellsGlossina austeniPrimary symbiontSecondary symbiontsSymbiotic associationDistinct lineagesRibosomal DNAGenus WolbachiaSymbiotic organismsTaxonomic placementΓ-subdivisionITS-2Third organismMidgut cellsAfrican trypanosomes
1997
Prevention of insect-borne disease: An approach using transgenic symbiotic bacteria
Durvasula R, Gumbs A, Panackal A, Kruglov O, Aksoy S, Merrifield R, Richards F, Beard C. Prevention of insect-borne disease: An approach using transgenic symbiotic bacteria. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 3274-3278. PMID: 9096383, PMCID: PMC20359, DOI: 10.1073/pnas.94.7.3274.Peer-Reviewed Original ResearchConceptsSymbiotic bacteriaDisease-transmitting insectsChagas disease vectorsSymbiont acquisitionTransgenic bacteriaInsect-borne diseaseR. prolixusDisease vectorsCertain arthropodsRhodnius prolixusT. cruziTrypanosoma cruziCecropin AExpression of moleculesInsectsBacteriaPowerful approachProlixusCruziExpressionEndosymbiontsArthropodsCecropinAntiparasitic activityParasites
1993
Phylogenetically distant symbiotic microorganisms reside in Glossina midgut and ovary tissues
O'NEILL S, GOODING R, AKSOY S. Phylogenetically distant symbiotic microorganisms reside in Glossina midgut and ovary tissues. Medical And Veterinary Entomology 1993, 7: 377-383. PMID: 8268495, DOI: 10.1111/j.1365-2915.1993.tb00709.x.Peer-Reviewed Original ResearchConceptsIntracellular bacterial symbiontsBlood-feeding insectsDNA oligonucleotide primersBacterial symbiontsSymbiotic microorganismsAlpha subdivisionSymbiotic bacteriaGamma subdivisionGenus WolbachiaGlossina speciesPolymerase chain reactionOvary tissuesOligonucleotide primersTsetse fliesMidgutSpeciesSymbiontsInsectsWolbachiaProteobacteriaSubspeciesChain reactionFliesMicroorganismsTissue