2021
Infection with endosymbiotic Spiroplasma disrupts tsetse (Glossina fuscipes fuscipes) metabolic and reproductive homeostasis
Son JH, Weiss BL, Schneider DI, Dera KM, Gstöttenmayer F, Opiro R, Echodu R, Saarman NP, Attardo GM, Onyango M, Abdalla A, Aksoy S. Infection with endosymbiotic Spiroplasma disrupts tsetse (Glossina fuscipes fuscipes) metabolic and reproductive homeostasis. PLOS Pathogens 2021, 17: e1009539. PMID: 34529715, PMCID: PMC8478229, DOI: 10.1371/journal.ppat.1009539.Peer-Reviewed Original ResearchConceptsReproductive fitnessSpiroplasma infectionSex-biased gene expressionHigh-throughput RNA sequencingReproductive physiologyIntrauterine larval developmentMale reproductive fitnessPathogenic African trypanosomesEndosymbiotic bacteriaFly resistanceTsetse fecundityFemale spermathecaFemale fecundityRNA sequencingLarval developmentSpiroplasmaGene expressionAfrican trypanosomesReproductive tissuesReproductive homeostasisTsetse hostHuman diseasesPopulation sizeProtective phenotypeLab lines
2020
Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens
Vigneron A, O'Neill MB, Weiss BL, Savage AF, Campbell OC, Kamhawi S, Valenzuela JG, Aksoy S. Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 2613-2621. PMID: 31964820, PMCID: PMC7007551, DOI: 10.1073/pnas.1914423117.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingRNA sequencingInfectious metacyclic formsMetacyclic parasitesMammalian host environmentFly salivary glandsMajor cell clustersClustering of cellsTsetse salivary glandsFamily proteinsDevelopmental programMammalian hostsMetacyclic cellsProtein transcriptsTrypanosoma bruceiDevelopmental processesGene expressionAfrican trypanosomesExpression profilesMolecular mechanismsSalivary glandsNew hostSurface localizationTrypanosome transmissionMetacyclogenesis
2019
Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes
Attardo GM, Abd-Alla AMM, Acosta-Serrano A, Allen JE, Bateta R, Benoit JB, Bourtzis K, Caers J, Caljon G, Christensen MB, Farrow DW, Friedrich M, Hua-Van A, Jennings EC, Larkin DM, Lawson D, Lehane MJ, Lenis VP, Lowy-Gallego E, Macharia RW, Malacrida AR, Marco HG, Masiga D, Maslen GL, Matetovici I, Meisel RP, Meki I, Michalkova V, Miller WJ, Minx P, Mireji PO, Ometto L, Parker AG, Rio R, Rose C, Rosendale AJ, Rota-Stabelli O, Savini G, Schoofs L, Scolari F, Swain MT, Takáč P, Tomlinson C, Tsiamis G, Van Den Abbeele J, Vigneron A, Wang J, Warren WC, Waterhouse RM, Weirauch MT, Weiss BL, Wilson RK, Zhao X, Aksoy S. Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes. Genome Biology 2019, 20: 187. PMID: 31477173, PMCID: PMC6721284, DOI: 10.1186/s13059-019-1768-2.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNA Transposable ElementsDrosophila melanogasterFemaleGene Expression RegulationGenes, InsectGenes, X-LinkedGenome, InsectGenomicsGeographyInsect ProteinsInsect VectorsMaleMutagenesis, InsertionalPhylogenyRepetitive Sequences, Nucleic AcidSequence Homology, Amino AcidSyntenyTrypanosomaTsetse FliesWolbachiaConceptsFemale-specific gene expressionMale seminal proteinsSex-linked scaffoldsComparative genomic analysisLow evolutionary ratesVectors of humanSyntenic analysisEvolutionary ratesNovel pestsEvolutionary relationshipsBacterial symbiosisGustatory genesEvolutionary biologyHelicase activityStructural conservationDifferent habitatsSeminal proteinsGenomic analysisHost preferenceX chromosomeDisease control strategiesUnique adaptationsGene expressionAfrican trypanosomesRhodopsin gene
2007
Refractoriness in Tsetse Flies (Diptera: Glossinidae) May be a Matter of Timing
Nayduch D, Aksoy S. Refractoriness in Tsetse Flies (Diptera: Glossinidae) May be a Matter of Timing. Journal Of Medical Entomology 2007, 51 DOI: 10.1603/0022-2585(2007)44[660:ritfdg]2.0.co;2.Peer-Reviewed Original ResearchAttacin expressionTsetse fliesReverse geneticsRNA interferenceFly speciesGene expressionAttacinCertain speciesSusceptible speciesBlood feedingSpeciesVector competenceFliesImmune challengeEfficient vectorDevastating diseaseExpressionRefractory speciesTsetseG. pallidipesAfrican trypanosomiasisHumoral immune responseSalivary glandsRepressionDisease transmissionRefractoriness in Tsetse Flies (Diptera: Glossinidae) May be a Matter of Timing
Nayduch D, Aksoy S. Refractoriness in Tsetse Flies (Diptera: Glossinidae) May be a Matter of Timing. Journal Of Medical Entomology 2007, 44: 660-665. DOI: 10.1603/0022-2585%282007%2944%5b660%3aritfdg%5d2.0.co%3b2.Peer-Reviewed Original ResearchAttacin expressionTsetse fliesReverse geneticsRNA interferenceFly speciesGene expressionAttacinCertain speciesSusceptible speciesBlood feedingSpeciesVector competenceFliesImmune challengeEfficient vectorSusceptible fliesDevastating diseaseExpressionRefractory speciesTsetseG. pallidipesAfrican trypanosomiasisHumoral immune responseSalivary glandsRepression