2021
Infection with endosymbiotic Spiroplasma disrupts tsetse (Glossina fuscipes fuscipes) metabolic and reproductive homeostasis
Son JH, Weiss BL, Schneider DI, Dera KM, Gstöttenmayer F, Opiro R, Echodu R, Saarman NP, Attardo GM, Onyango M, Abdalla A, Aksoy S. Infection with endosymbiotic Spiroplasma disrupts tsetse (Glossina fuscipes fuscipes) metabolic and reproductive homeostasis. PLOS Pathogens 2021, 17: e1009539. PMID: 34529715, PMCID: PMC8478229, DOI: 10.1371/journal.ppat.1009539.Peer-Reviewed Original ResearchConceptsReproductive fitnessSpiroplasma infectionSex-biased gene expressionHigh-throughput RNA sequencingReproductive physiologyIntrauterine larval developmentMale reproductive fitnessPathogenic African trypanosomesEndosymbiotic bacteriaFly resistanceTsetse fecundityFemale spermathecaFemale fecundityRNA sequencingLarval developmentSpiroplasmaGene expressionAfrican trypanosomesReproductive tissuesReproductive homeostasisTsetse hostHuman diseasesPopulation sizeProtective phenotypeLab lines
2020
Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens
Vigneron A, O'Neill MB, Weiss BL, Savage AF, Campbell OC, Kamhawi S, Valenzuela JG, Aksoy S. Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 2613-2621. PMID: 31964820, PMCID: PMC7007551, DOI: 10.1073/pnas.1914423117.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingRNA sequencingInfectious metacyclic formsMetacyclic parasitesMammalian host environmentFly salivary glandsMajor cell clustersClustering of cellsTsetse salivary glandsFamily proteinsDevelopmental programMammalian hostsMetacyclic cellsProtein transcriptsTrypanosoma bruceiDevelopmental processesGene expressionAfrican trypanosomesExpression profilesMolecular mechanismsSalivary glandsNew hostSurface localizationTrypanosome transmissionMetacyclogenesis
2019
Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes
Attardo GM, Abd-Alla AMM, Acosta-Serrano A, Allen JE, Bateta R, Benoit JB, Bourtzis K, Caers J, Caljon G, Christensen MB, Farrow DW, Friedrich M, Hua-Van A, Jennings EC, Larkin DM, Lawson D, Lehane MJ, Lenis VP, Lowy-Gallego E, Macharia RW, Malacrida AR, Marco HG, Masiga D, Maslen GL, Matetovici I, Meisel RP, Meki I, Michalkova V, Miller WJ, Minx P, Mireji PO, Ometto L, Parker AG, Rio R, Rose C, Rosendale AJ, Rota-Stabelli O, Savini G, Schoofs L, Scolari F, Swain MT, Takáč P, Tomlinson C, Tsiamis G, Van Den Abbeele J, Vigneron A, Wang J, Warren WC, Waterhouse RM, Weirauch MT, Weiss BL, Wilson RK, Zhao X, Aksoy S. Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes. Genome Biology 2019, 20: 187. PMID: 31477173, PMCID: PMC6721284, DOI: 10.1186/s13059-019-1768-2.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNA Transposable ElementsDrosophila melanogasterFemaleGene Expression RegulationGenes, InsectGenes, X-LinkedGenome, InsectGenomicsGeographyInsect ProteinsInsect VectorsMaleMutagenesis, InsertionalPhylogenyRepetitive Sequences, Nucleic AcidSequence Homology, Amino AcidSyntenyTrypanosomaTsetse FliesWolbachiaConceptsFemale-specific gene expressionMale seminal proteinsSex-linked scaffoldsComparative genomic analysisLow evolutionary ratesVectors of humanSyntenic analysisEvolutionary ratesNovel pestsEvolutionary relationshipsBacterial symbiosisGustatory genesEvolutionary biologyHelicase activityStructural conservationDifferent habitatsSeminal proteinsGenomic analysisHost preferenceX chromosomeDisease control strategiesUnique adaptationsGene expressionAfrican trypanosomesRhodopsin gene
2016
Mammalian African trypanosome VSG coat enhances tsetse’s vector competence
Aksoy E, Vigneron A, Bing X, Zhao X, O'Neill M, Wu YN, Bangs JD, Weiss BL, Aksoy S. Mammalian African trypanosome VSG coat enhances tsetse’s vector competence. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: 6961-6966. PMID: 27185908, PMCID: PMC4922192, DOI: 10.1073/pnas.1600304113.Peer-Reviewed Original ResearchConceptsVariant surface glycoproteinPeritrophic matrixMammalian hostsVector competenceTranscription factor familyMidgut homeostasisTsetse midgutTrypanosome biologyFactor familyPM barrierCoat proteinNovel functionAfrican trypanosomesTsetse vectorInfection processParasite developmentAnimal trypanosomiasesAntigenic variationVSG moleculesVSG coatBiological vectorsMidgutProtozoan parasiteDisease transmissionTsetse flies
2007
Novel strategies targeting pathogen transmission reduction in insect vectors: Tsetse‐transmitted trypanosomiasis control
WEISS B, ATTARDO G, Roshan P, Jingwen W, AKSOY S. Novel strategies targeting pathogen transmission reduction in insect vectors: Tsetse‐transmitted trypanosomiasis control. Entomological Research 2007, 37: 231-237. DOI: 10.1111/j.1748-5967.2007.00119.x.Peer-Reviewed Original ResearchInsect vectorsEffective disease management strategiesNovel disease control strategiesDisease management strategiesInsect-pathogen interactionsDisease control strategiesPopulation reduction methodsAgricultural diseasesTransgenic technologyTrypanosomiasis controlImportant human diseasesInsect biologyManagement strategiesTsetse vectorVector populationsAfrican trypanosomesControl strategyHuman diseasesDisease controlInsectsRecent knowledgeVector control strategiesBiologyCropsSelective elimination
2001
Tsetse immune responses and trypanosome transmission: Implications for the development of tsetse-based strategies to reduce trypanosomiasis
Hao Z, Kasumba I, Lehane M, Gibson W, Kwon J, Aksoy S. Tsetse immune responses and trypanosome transmission: Implications for the development of tsetse-based strategies to reduce trypanosomiasis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 12648-12653. PMID: 11592981, PMCID: PMC60108, DOI: 10.1073/pnas.221363798.Peer-Reviewed Original ResearchConceptsFat bodyInsect immune systemAntimicrobial peptide genesFat body tissueImmune responsive genesDifferent life stagesTransgenic approachesResponsive genesAntimicrobial genesImmune responseSubtractive cloningPeptide genesAfrican trypanosomesMolecular signalsImmune systemDifferential regulationInfection processLife stagesTrypanosome transmissionParasite transmissionGenesImportant vectorEscherichia coliBiochemical natureDiptericinMolecular characterization of two serine proteases expressed in gut tissue of the African trypanosome vector, Glossina morsitans morsitans
Yan J, Cheng Q, Li C, Aksoy S. Molecular characterization of two serine proteases expressed in gut tissue of the African trypanosome vector, Glossina morsitans morsitans. Insect Molecular Biology 2001, 10: 47-56. PMID: 11240636, DOI: 10.1046/j.1365-2583.2001.00232.x.Peer-Reviewed Original ResearchConceptsSerine proteasesSerine protease-encoding genePutative mature proteinHydrophobic signal peptide sequenceInvertebrate serine proteasesProtease-encoding genesPost-transcriptional levelAmino acid mature peptideGut tissueSer catalytic triadSignal peptide sequenceRegulation of expressionPattern of expressionSerine protease 1Insect gutMature proteinVector insectsDeduced peptideChymotrypsin-like proteaseTsetse genomeSpecific residuesCatalytic triadAfrican trypanosomesMature peptidePathogen establishment
1999
Concordant Evolution of a Symbiont with Its Host Insect Species: Molecular Phylogeny of Genus Glossina and Its Bacteriome-Associated Endosymbiont, Wigglesworthia glossinidia
Chen X, Li S, Aksoy S. Concordant Evolution of a Symbiont with Its Host Insect Species: Molecular Phylogeny of Genus Glossina and Its Bacteriome-Associated Endosymbiont, Wigglesworthia glossinidia. Journal Of Molecular Evolution 1999, 48: 49-58. PMID: 9873076, DOI: 10.1007/pl00006444.Peer-Reviewed Original ResearchConceptsHost insect speciesRDNA sequence analysisInsect speciesGroup speciesSequence analysisSister-group relationshipSpacer 2 (ITS2) regionParasitic African trypanosomesWigglesworthia glossinidiaMolecular phylogenyDifferentiated epithelial cellsGlossina austeniPrimary symbiontSecondary symbiontsSymbiotic associationDistinct lineagesRibosomal DNAGenus WolbachiaSymbiotic organismsTaxonomic placementΓ-subdivisionITS-2Third organismMidgut cellsAfrican trypanosomes
1992
Spliced leader RNA sequences of Trypanosoma rangeli are organized within the 5S rRNA-encoding genes
Aksoy S, Shay G, Villaneuva M, Beard C, Richards F. Spliced leader RNA sequences of Trypanosoma rangeli are organized within the 5S rRNA-encoding genes. Gene 1992, 113: 239-243. PMID: 1572544, DOI: 10.1016/0378-1119(92)90401-a.Peer-Reviewed Original Research