Application of a Modified Smart-seq2 Sample Preparation Protocol for Rare Cell Full-length Single-cell mRNA Sequencing to Mouse Oocytes.
Treger RS, Pope SD, Xing X, Iwasaki A. Application of a Modified Smart-seq2 Sample Preparation Protocol for Rare Cell Full-length Single-cell mRNA Sequencing to Mouse Oocytes. Bio-protocol 2019, 9: e3345. PMID: 33654848, PMCID: PMC7854224, DOI: 10.21769/bioprotoc.3345.Peer-Reviewed Original ResearchEndogenous retrovirusesFull-length cDNA libraryRNA sequencing librariesSmart-seq2 protocolSingle-cell mRNALibrary preparation methodsMurine genomeCDNA librarySequencing librariesExpression analysisERV expressionPrecise regulationGerm cellsIsolate populationMouse oocytesSubsequent sequencingOocytesMature oocytesSample preparation protocolFamily membersRetroelementsGenomeEmbryogenesisLibraryPreparation protocol