2017
Pharmacokinetics of Miltefosine in Children and Adults with Cutaneous Leishmaniasis
del Mar Castro M, Gomez MA, Kip AE, Cossio A, Ortiz E, Navas A, Dorlo TP, Saravia NG. Pharmacokinetics of Miltefosine in Children and Adults with Cutaneous Leishmaniasis. Antimicrobial Agents And Chemotherapy 2017, 61: 10.1128/aac.02198-16. PMID: 27956421, PMCID: PMC5328512, DOI: 10.1128/aac.02198-16.Peer-Reviewed Original ResearchConceptsCutaneous leishmaniasisMiltefosine concentrationsPeripheral blood mononuclear cellsInitiation of treatmentCompletion of treatmentBlood mononuclear cellsEnd of treatmentOutcome of treatmentConcentration-time curvePharmacokinetic clinical trialsClinical responsePediatric patientsPediatric populationMononuclear cellsTherapeutic regimensDrug exposureClinical trialsNoncompartmental analysisParasite eliminationTherapeutic outcomesStudy participantsMiltefosinePatientsLiquid chromatography-tandem mass spectrometryPK differences
2014
CD4 T cell activation by B cells in human Leishmania (Viannia)infection
Rodriguez-Pinto D, Saravia NG, McMahon-Pratt D. CD4 T cell activation by B cells in human Leishmania (Viannia)infection. BMC Infectious Diseases 2014, 14: 108. PMID: 24568275, PMCID: PMC3937821, DOI: 10.1186/1471-2334-14-108.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedBiotinB-LymphocytesCD4-Positive T-LymphocytesColombiaFemaleFlow CytometryFluorescein-5-isothiocyanateGene Expression RegulationHumansImmunoglobulin MInterferon-gammaInterleukin-6Leishmania braziliensisLeishmaniasis, CutaneousLeukocytes, MononuclearLymphocyte ActivationMaleMiddle AgedOvalbuminTumor Necrosis Factor-alphaYoung AdultConceptsCD4 T cellsCutaneous leishmaniasis patientsT cellsB cellsLeishmaniasis patientsT cell activationLeishmania antigenImmune responseCell activationT-cell activation parametersSpecific CD4 T cellsEffective adaptive immune responseCD4 T cell activationB-cell activation markersCultures of PBMCUpregulation of CD86Cell activation markersCostimulatory molecule CD86Activation markers CD25Adaptive immune responsesHuman cutaneous leishmaniasisPurified B cellsT cell culturesHuman B cell linesHuman B cells
1997
Carbohydrate and LPG expression in Leishmania viannia subgenus.
Muskus C, Segura I, Oddone R, Turco S, Leiby D, Toro L, Robledo S, Saravia N. Carbohydrate and LPG expression in Leishmania viannia subgenus. Journal Of Parasitology 1997, 83: 671-8. PMID: 9267410, DOI: 10.2307/3284245.Peer-Reviewed Original ResearchConceptsGrowth phaseLogarithmic phase promastigotesRepeat unitsViannia subgenusLPG repeat unitsSpecies referenceL. donovani lipophosphoglycanLPG expressionMolecular determinantsSubgeneraCell surfaceGlycosylated moleculesStationary phase promastigotesL. donovaniDistinct populationsBiochemical analysisExpression of carbohydratesSpeciesExpressionL. panamensisL. guyanensisSuch moleculesL. braziliensisLeishmania promastigotesFlow cytometry
1993
Epidemiology of Cutaneous Leishmaniasis in Colombia: A Longitudinal Study of the Natural History, Prevalence, and Incidence of Infection and Clinical Manifestations
Weigle K, Santrich C, Martinez F, Valderrama L, Saravia N. Epidemiology of Cutaneous Leishmaniasis in Colombia: A Longitudinal Study of the Natural History, Prevalence, and Incidence of Infection and Clinical Manifestations. The Journal Of Infectious Diseases 1993, 168: 699-708. PMID: 8354912, DOI: 10.1093/infdis/168.3.699.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAge FactorsAnimalsAntigens, ProtozoanChildChild, PreschoolCohort StudiesColombiaFemaleHumansIncidenceInfantInfant, NewbornLeishmania braziliensisLeishmaniasis, CutaneousLongitudinal StudiesMaleMiddle AgedModels, BiologicalProspective StudiesRespiratory SystemRural PopulationSex FactorsSkinSkin TestsTime FactorsConceptsLeishmanin skin test conversionMost primary infectionsSkin test conversionBurden of diseaseIncidence of infectionLeishmaniasis control programClinical manifestationsProspective studyPrimary infectionTest conversionLeishmania infectionIncidence rateTypical scarCutaneous leishmaniasisEndemic areasNew infectionsLeishmaniasis casesNatural historyInfectionLeishmaniasisNaive hostsLongitudinal studyLesionsOne-thirdIncidenceEpidemiology of Cutaneous Leishmaniasis in Colombia: Environmental and Behavioral Risk Factors for Infection, Clinical Manifestations, and Pathogenicity
Weigle K, Santrich C, Martinez F, Valderrama L, Saravia N. Epidemiology of Cutaneous Leishmaniasis in Colombia: Environmental and Behavioral Risk Factors for Infection, Clinical Manifestations, and Pathogenicity. The Journal Of Infectious Diseases 1993, 168: 709-714. PMID: 8354913, DOI: 10.1093/infdis/168.3.709.Peer-Reviewed Original ResearchConceptsRisk factorsFarming occupationLeishmania panamensis infectionRisk factor informationBehavioral risk factorsAcquisition of infectionYears of ageRisk of lesionsPanamensis infectionLeishmanial lesionsClinical manifestationsMale sexActive surveillanceLeishmania infectionCase ascertainmentCutaneous leishmaniasisNew infectionsInfectionLeishmania braziliensisLesionsFactor informationStrongest predictorAgeLeishmaniasisSexLeishmania (Viannia) panamensis-specific IgE and IgA antibodies in relation to expression of human tegumentary leishmaniasis.
O'Neil C, Labrada M, Saravia N. Leishmania (Viannia) panamensis-specific IgE and IgA antibodies in relation to expression of human tegumentary leishmaniasis. American Journal Of Tropical Medicine And Hygiene 1993, 49: 181-8. PMID: 8357080, DOI: 10.4269/ajtmh.1993.49.181.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAnimalsAntibodies, ProtozoanAntibody SpecificityAntigens, ProtozoanChildChild, PreschoolCross ReactionsDose-Response Relationship, ImmunologicEnzyme-Linked Immunosorbent AssayFemaleHumansImmunoglobulin AImmunoglobulin EInfantLeishmania braziliensisLeishmaniasis, CutaneousLeishmaniasis, MucocutaneousMaleRadioallergosorbent TestRetrospective StudiesConceptsAmerican tegumentary leishmaniasisIgA antibodiesEnzyme-linked immunosorbent assayTegumentary leishmaniasisRadioallergosorbent testRAST valuesHuman tegumentary leishmaniasisDuration of diseaseIgA levelsMucocutaneous diseaseBiopsy specimenBiopsy specimensCutaneous diseaseSevere formPatientsHeterologous antigensDisease evolutionIgEImmunosorbent assayElevated levelsLeishmaniasisDiseaseInhibition testingAntibodiesSeparate groups
1992
Haemoculture of Leishmania (Viannia) braziliensis from two cases of mucosal leishmaniasis: Re-examination of haematogenous dissemination
Martinez J, Alba, Arias L, Escobar M, Saravia N. Haemoculture of Leishmania (Viannia) braziliensis from two cases of mucosal leishmaniasis: Re-examination of haematogenous dissemination. Transactions Of The Royal Society Of Tropical Medicine And Hygiene 1992, 86: 392-394. PMID: 1440813, DOI: 10.1016/0035-9203(92)90233-3.Peer-Reviewed Original ResearchMeSH KeywordsAdultAnimalsHumansLeishmania braziliensisLeishmaniasis, MucocutaneousLeukocytes, MononuclearMaleMiddle AgedConceptsMucosal leishmaniasisActive pulmonary tuberculosisMontenegro skin testPeripheral blood leucocytesIsolation of parasitesIndirect fluorescent antibodyCases of leishmaniasisCulture of parasitesHaematogenous disseminationMucosal involvementPulmonary tuberculosisCutaneous lesionsSkin testMucosal diseaseMetastatic spreadMonths durationBlood leucocytesRight legPatientsLeishmania parasitesLeishmania braziliensisL. braziliensisLeishmaniasisFluorescent antibodyLeucocytes
1991
Metastatic capability of Leishmania (Viannia) panamensis and Leishmania (Viannia) guyanensis in golden hamsters.
Martinez J, Travi B, Valencia A, Saravia N. Metastatic capability of Leishmania (Viannia) panamensis and Leishmania (Viannia) guyanensis in golden hamsters. Journal Of Parasitology 1991, 77: 762-8. PMID: 1919926, DOI: 10.2307/3282713.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone MarrowCricetinaeLeishmania braziliensisLeishmaniasis, MucocutaneousLiverLymph NodesMaleMesocricetusSkinSpleenTime FactorsConceptsCutaneous metastatic lesionsMetastatic lesionsFrequency of isolationLymph nodesL. panamensisL. guyanensisLeishmania panamensisLeishmania guyanensisGolden hamstersDistant lymph nodesCutaneous metastasesPrimary lesionLymphatic disseminationExperimental infectionLesionsMetastatic capabilityHuman casesTissue samplesHamstersParasite culturesSandfly vectorNineteen strainsInoculation siteGuyanensisInoculum doseCorrelation between histopathology, immune response, clinical presentation, and evolution in Leishmania braziliensis infection.
Gutierrez Y, Salinas G, Palma G, Valderrama L, Santrich C, Saravia N. Correlation between histopathology, immune response, clinical presentation, and evolution in Leishmania braziliensis infection. American Journal Of Tropical Medicine And Hygiene 1991, 45: 281-9. PMID: 1928562, DOI: 10.4269/ajtmh.1991.45.281.Peer-Reviewed Original ResearchConceptsPresence of amastigotesImmune responseClinical presentationLeishmania-specific immune responseAbsence of amastigotesPresence of epithelioidLymphocyte transformation responsesPresence of granulomasRole of antibodiesCellular immune responsesPresence of eosinophilsSerum antibody titersLeishmania braziliensis infectionPresence of LeishmaniaLeishmanial lesionsBraziliensis infectionCellular infiltrateType hypersensitivityClinical parametersActive lesionsAntibody titersEvolution of diseaseSoluble mediatorsTegumentary leishmaniasisEpitheloid cellsLeishmanin skin test standardization and evaluation of safety, dose, storage, longevity of reaction and sensitization.
Weigle K, Valderrama L, Arias A, Santrich C, Saravia N. Leishmanin skin test standardization and evaluation of safety, dose, storage, longevity of reaction and sensitization. American Journal Of Tropical Medicine And Hygiene 1991, 44: 260-71. PMID: 2035747, DOI: 10.4269/ajtmh.1991.44.260.Peer-Reviewed Original Research
1990
Infectivity of the subspecies of the Leishmania braziliensis complex in vivo and in vitro.
Rey J, Travi B, Valencia A, Saravia N. Infectivity of the subspecies of the Leishmania braziliensis complex in vivo and in vitro. American Journal Of Tropical Medicine And Hygiene 1990, 43: 623-31. PMID: 2267967, DOI: 10.4269/ajtmh.1990.43.623.Peer-Reviewed Original ResearchRecurrent lesions in human Leishmania braziliensis infection—reactivation or reinfection?
Saravia N, Segura I, Labrada L, Weigle K, Giannini S, Pacheco R, Goncalves A. Recurrent lesions in human Leishmania braziliensis infection—reactivation or reinfection? The Lancet 1990, 336: 398-402. PMID: 1974943, DOI: 10.1016/0140-6736(90)91945-7.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDNAHumansKaryotypingLeishmaniaLeishmania braziliensisLeishmaniasisPolymorphism, GeneticRecurrenceConceptsRecurrent lesionsInitial lesionLonger disease-free intervalDisease-free intervalAmerican tegumentary leishmaniasisRecurrent diseaseEndogenous reactivationExogenous reinfectionVaccination strategiesClinical dataPatientsTegumentary leishmaniasisLesionsTreatment evaluationRecurrent strainsLeishmania braziliensisIdentical strainsLatter groupReinfectionRestriction endonuclease analysisEndonuclease analysisStrain pairsMolecular karyotypingKaryotype profilePolymorphismMucosal disease caused by Leishmania braziliensis guyanensis.
Santrich C, Segura I, Arias A, Saravia N. Mucosal disease caused by Leishmania braziliensis guyanensis. American Journal Of Tropical Medicine And Hygiene 1990, 42: 51-5. PMID: 2301705, DOI: 10.4269/ajtmh.1990.42.51.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsColombiaCricetinaeHumansLeishmaniaLeishmania braziliensisLeishmaniasis, MucocutaneousMaleMiddle AgedPhenotype
1989
Evaluación de la respuesta de isotipos de inmunoglobulina especifica a Leishmania en leishmaniasis tegumentaria Americana
Labrada M, Weigle K, Valderrama L, Saravia N. Evaluación de la respuesta de isotipos de inmunoglobulina especifica a Leishmania en leishmaniasis tegumentaria Americana. Memórias Do Instituto Oswaldo Cruz 1989, 84: 409-416. PMID: 2520833, DOI: 10.1590/s0074-02761989000300018.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, ProtozoanFluorescent Antibody TechniqueHumansImmunoglobulin IsotypesIntradermal TestsLeishmania braziliensisLeishmaniasis, CutaneousConceptsIndirect immunofluorescent antibody testSpecific IgMMonths durationImmunoglobulin subclass responsesLeishmania-specific IgGNormal control volunteersMontenegro skin testTime of recurrenceStaphylococcus aureus CowanImmunofluorescent antibody testL. mexicana amazonensisLeishmania antigenSubclass responsesSkin testingIgM titersSkin testActive lesionsIgG reactiveSpecific IgGSpecific IgG.Control volunteersComplete healingEarly lesionsAntibody testPatientsThe Relationship of Leishmania braziliensis Subspecies and Immune Response to Disease Expression in New World Leishmaniasis
Saravia N, Valderrama L, Labrada M, Holguin A, Navas C, Palma G, Weigle K. The Relationship of Leishmania braziliensis Subspecies and Immune Response to Disease Expression in New World Leishmaniasis. The Journal Of Infectious Diseases 1989, 159: 725-735. PMID: 2647862, DOI: 10.1093/infdis/159.4.725.Peer-Reviewed Original ResearchMeSH KeywordsAnalysis of VarianceAnimalsAntibodies, ProtozoanAntigens, ProtozoanDose-Response Relationship, ImmunologicFluorescent Antibody TechniqueHost-Parasite InteractionsHumansHypersensitivity, DelayedImmune SeraImmunity, CellularKineticsLeishmaniaLeishmania braziliensisLeishmaniasisLeishmaniasis, MucocutaneousLymphocyte ActivationRegression AnalysisConceptsCutaneous DTHLeishmania antigenBraziliensis infectionTegumentary leishmaniasisIFAT titersDisease formsVitro lymphocyte proliferative responseCutaneous DTH responseHuman tegumentary leishmaniasisL. braziliensis infectionLymphocyte proliferative responsesImmune response parametersHigher IFAT titersNew World leishmaniasisPanamensis infectionDTH responseMucocutaneous diseaseClinical presentationLesion durationCutaneous diseaseDisease presentationImmune mechanismsImmune responseProliferative responseTime of evolution
1988
Behavior of Leishmania braziliensis s.l. in golden hamsters: evolution of the infection under different experimental conditions.
Travi B, Rey-Ladino J, Saravia N. Behavior of Leishmania braziliensis s.l. in golden hamsters: evolution of the infection under different experimental conditions. Journal Of Parasitology 1988, 74: 1059-62. PMID: 3193329, DOI: 10.2307/3282237.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCricetinaeHydrocortisoneLeishmaniaLeishmania braziliensisLeishmaniasisLymph NodesMesocricetus