2018
Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Assay for Diagnosis of Cutaneous and Visceral Leishmaniasis
Adams ER, Schoone G, Versteeg I, Gomez MA, Diro E, Mori Y, Perlee D, Downing T, Saravia N, Assaye A, Hailu A, Albertini A, Ndung'u JM, Schallig H. Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Assay for Diagnosis of Cutaneous and Visceral Leishmaniasis. Journal Of Clinical Microbiology 2018, 56: 10.1128/jcm.00386-18. PMID: 29695527, PMCID: PMC6018344, DOI: 10.1128/jcm.00386-18.Peer-Reviewed Original ResearchConceptsVisceral leishmaniasisLAMP assayDiagnostic accuracyProspective cohort trialDiagnosis of CLBone marrow aspirateMinicircle kinetoplast DNANear-patient settingIsothermal Amplification AssayCohort trialVL patientsCL diagnosisVL suspectsMarrow aspiratesMalaria parasitesSwab samplesDiagnosisWhole bloodGold standardInvasive samplesAnalytical specificityClinical samplesAmplification assaysPatientsReference standard
2006
Resistance to Antimony and Treatment Failure in Human Leishmania (Viannia) Infection
Rojas R, Valderrama L, Valderrama M, Varona MX, Ouellette M, Saravia NG. Resistance to Antimony and Treatment Failure in Human Leishmania (Viannia) Infection. The Journal Of Infectious Diseases 2006, 193: 1375-1383. PMID: 16619185, DOI: 10.1086/503371.Peer-Reviewed Original ResearchConceptsTreatment failureMeglumine antimoniatePrimary resistanceAntimonial drugsSecondary resistanceDrug resistanceHuman Leishmania infectionsEffective immune responseAnthroponotic visceral leishmaniasisAmerican cutaneous leishmaniasisAntimonial therapyStandard treatmentCutaneous diseaseLeishmania infectionTherapeutic responseImmune responseCutaneous leishmaniasisVisceral leishmaniasisAnthroponotic transmissionResistant organismsEffective doseIntracellular amastigotesSusceptible strainsAntimoniatePatients