2016
An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections
Saldarriaga OA, Castellanos-Gonzalez A, Porrozzi R, Baldeviano GC, Lescano AG, de Los Santos MB, Fernandez OL, Saravia NG, Costa E, Melby PC, Travi BL. An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections. PLOS Neglected Tropical Diseases 2016, 10: e0004638. PMID: 27115155, PMCID: PMC4845993, DOI: 10.1371/journal.pntd.0004638.Peer-Reviewed Original ResearchConceptsMucosal leishmaniasisDiagnostic testsL. braziliensisPrimary health systemL. panamensisRPA-LFSensitive diagnostic testMost endemic regionsMolecular diagnostic testsChronic lesionsReal-time PCRCutaneous leishmaniasisEndemic regionsParasite burdenHealth systemClinical validationLeishmaniasisL. naiffiMolecular testsParasite DNALeishmania sppTime PCRClinical samplesViannia subgenusRecombinase polymerase amplification
2000
INTRASPECIFIC HETEROGENEITY IN THE MINI-EXON GENE LOCALIZATION OF LEISHMANIA (VIANNIA) PANAMENSIS AND LEISHMANIA (VIANNIA) GUYANENSIS FROM COLOMBIA
Pacheco R, Fernandes O, Salinas G, Segura I, Momen H, Degrave W, Saravia N, Campbell D. INTRASPECIFIC HETEROGENEITY IN THE MINI-EXON GENE LOCALIZATION OF LEISHMANIA (VIANNIA) PANAMENSIS AND LEISHMANIA (VIANNIA) GUYANENSIS FROM COLOMBIA. Journal Of Parasitology 2000, 86: 1250-1253. PMID: 11191900, DOI: 10.1645/0022-3395(2000)086[1250:ihitme]2.0.co;2.Peer-Reviewed Original ResearchConceptsMini-exon gene sequencesGene sequencesGene localizationMini-exon geneChromosomal localizationGenetic variabilityL. guyanensisIntraspecific heterogeneityDifferent karyotypesChromosomesPanamensisKbL. panamensisSpeciesLeishmania panamensisMolecular epidemiologic markerViannia subgenusHuman isolates
1997
Carbohydrate and LPG expression in Leishmania viannia subgenus.
Muskus C, Segura I, Oddone R, Turco S, Leiby D, Toro L, Robledo S, Saravia N. Carbohydrate and LPG expression in Leishmania viannia subgenus. Journal Of Parasitology 1997, 83: 671-8. PMID: 9267410, DOI: 10.2307/3284245.Peer-Reviewed Original ResearchConceptsGrowth phaseLogarithmic phase promastigotesRepeat unitsViannia subgenusLPG repeat unitsSpecies referenceL. donovani lipophosphoglycanLPG expressionMolecular determinantsSubgeneraCell surfaceGlycosylated moleculesStationary phase promastigotesL. donovaniDistinct populationsBiochemical analysisExpression of carbohydratesSpeciesExpressionL. panamensisL. guyanensisSuch moleculesL. braziliensisLeishmania promastigotesFlow cytometry
1996
Leishmania RNA viruses in Leishmania of the Viannia subgenus.
Salinas G, Zamora M, Stuart K, Saravia N. Leishmania RNA viruses in Leishmania of the Viannia subgenus. American Journal Of Tropical Medicine And Hygiene 1996, 54: 425-9. PMID: 8615459, DOI: 10.4269/ajtmh.1996.54.425.Peer-Reviewed Original ResearchConceptsL. braziliensisL. braziliensis strainWorld Health Organization referenceViannia subgenusLeishmania RNA virusCapsid protein fragmentMucosal lesionsStrains of LeishmaniaEpidemiologic markersMetastatic traitsVirus presentKaryotype analysisMaterial reactiveColombian strainL. guyanensisVirusBraziliensisLeishmaniaLRV1Polyclonal antibodiesRNA virusesRecombinant fragmentsTotal lysatesReference strainsBiological characterization