Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Assay for Diagnosis of Cutaneous and Visceral Leishmaniasis
Adams ER, Schoone G, Versteeg I, Gomez MA, Diro E, Mori Y, Perlee D, Downing T, Saravia N, Assaye A, Hailu A, Albertini A, Ndung'u JM, Schallig H. Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Assay for Diagnosis of Cutaneous and Visceral Leishmaniasis. Journal Of Clinical Microbiology 2018, 56: 10.1128/jcm.00386-18. PMID: 29695527, PMCID: PMC6018344, DOI: 10.1128/jcm.00386-18.Peer-Reviewed Original ResearchConceptsVisceral leishmaniasisLAMP assayDiagnostic accuracyProspective cohort trialDiagnosis of CLBone marrow aspirateMinicircle kinetoplast DNANear-patient settingIsothermal Amplification AssayCohort trialVL patientsCL diagnosisVL suspectsMarrow aspiratesMalaria parasitesSwab samplesDiagnosisWhole bloodGold standardInvasive samplesAnalytical specificityClinical samplesAmplification assaysPatientsReference standard