2021
Potency and Preclinical Evidence of Synergy of Oral Azole Drugs and Miltefosine in an Ex Vivo Model of Leishmania (Viannia) panamensis Infection
Fernández OL, Rosales-Chilama M, Quintero N, Travi BL, Wetzel DM, Gómez MA, Saravia NG. Potency and Preclinical Evidence of Synergy of Oral Azole Drugs and Miltefosine in an Ex Vivo Model of Leishmania (Viannia) panamensis Infection. Antimicrobial Agents And Chemotherapy 2021, 66: e01425-21. PMID: 34694879, PMCID: PMC8765415, DOI: 10.1128/aac.01425-21.Peer-Reviewed Original ResearchConceptsFractional inhibitory concentration indexPeripheral blood mononuclear cellsL. panamensisHuman peripheral blood mononuclear cellsClinical strainsLeishmania panamensis infectionOral azole drugsOral combination therapyFailure of treatmentBlood mononuclear cellsEfficacy of treatmentNovel therapeutic strategiesEffectiveness of treatmentHigh potencyReduction of infectionEx vivo modelInhibitory concentration indexFree drug concentrationPanamensis infectionPreclinical evidenceCombination therapyMononuclear cellsAntimonial drugsCutaneous leishmaniasisDrug combinations
2012
Human Macrophage Response to L. (Viannia) panamensis: Microarray Evidence for an Early Inflammatory Response
Ramírez C, Díaz-Toro Y, Tellez J, Castilho TM, Rojas R, Ettinger NA, Tikhonova I, Alexander ND, Valderrama L, Hager J, Wilson ME, Lin A, Zhao H, Saravia NG, McMahon-Pratt D. Human Macrophage Response to L. (Viannia) panamensis: Microarray Evidence for an Early Inflammatory Response. PLOS Neglected Tropical Diseases 2012, 6: e1866. PMID: 23145196, PMCID: PMC3493378, DOI: 10.1371/journal.pntd.0001866.Peer-Reviewed Original ResearchConceptsMRNA abundance profilesMonocyte-derived macrophagesRegulation of genesDistinct biologic responsesAbundance profilesL. panamensisDifferential gene expressionEarly time pointsMacrophage responseEarly macrophage responseImmune responseLeishmania speciesHuman monocyte-derived macrophagesMicroarray evidenceOutset of infectionGene regulationActivation of PKCHuman macrophage responseGene expressionLeishmania panamensis infectionTime pointsCellular responsesAdaptive immune responsesG proteinsEarly inflammatory response
2009
Role of Toll-like receptors in the response of human macrophages to Leishmania panamensis infection (133.17)
Gallego C, Saravia N, Valderrama L, Golenbock D. Role of Toll-like receptors in the response of human macrophages to Leishmania panamensis infection (133.17). The Journal Of Immunology 2009, 182: 133.17-133.17. DOI: 10.4049/jimmunol.182.supp.133.17.Peer-Reviewed Original ResearchToll-like receptorsOutcome of infectionPanamensis infectionHuman macrophagesTLR ligandsL. panamensisExpression of TLR1L. panamensis infectionsLeishmania panamensis infectionActivation of TLR2Production of TNFαAmerican cutaneous leishmaniasisHuman primary macrophagesL. panamensis promastigotesPossible roleHealthy donorsCutaneous leishmaniasisTNFα productionInnate recognitionWild-type (WT) mouse macrophagesInfectionPrimary macrophagesTLR2MacrophagesPhagocytic cells
1998
Permissiveness of human monocytes and monocyte-derived macrophages to infection by promastigotes of Leishmania (Viannia) panamensis.
Bosque F, Milon G, Valderrama L, Saravia N. Permissiveness of human monocytes and monocyte-derived macrophages to infection by promastigotes of Leishmania (Viannia) panamensis. Journal Of Parasitology 1998, 84: 1250-6. PMID: 9920322, DOI: 10.2307/3284682.Peer-Reviewed Original ResearchConceptsPercentage of cellsBlood monocytesInduction of differentiationLeishmania panamensis infectionHuman peripheral blood monocytesHistiocytic cell line UDifferentiated macrophagesExpression of CD68Number of amastigotesPeripheral blood monocytesOutcome of infectionSite of infectionMonocyte-derived macrophagesL. panamensis promastigotesMononuclear phagocytic cellsPhorbol myristate acetatePanamensis infectionNumber of parasitesCell line UBone marrowFresh monocytesLeishmania panamensisMonocytesResident macrophagesStages of differentiation
1996
The inflammatory response promotes cutaneous metastasis in hamsters infected with Leishmania (Viannia) panamensis.
Travi B, Osorio Y, Saravia N. The inflammatory response promotes cutaneous metastasis in hamsters infected with Leishmania (Viannia) panamensis. Journal Of Parasitology 1996, 82: 454-7. PMID: 8636852, DOI: 10.2307/3284085.Peer-Reviewed Original ResearchConceptsInflammatory responseMetastatic lesionsControl animalsL. panamensisLeishmania panamensis infectionType hypersensitivity responseFrequency of metastasisNonspecific inflammatory responseDevelopment of lesionsExperimental groupElicitation of inflammationPanamensis infectionSkin metastasesCutaneous metastasesHind pawsSurgical excisionHypersensitivity responseSite of inductionIntradermal applicationSecondary diseaseHamster modelInflammatory stimuliLeishmania panamensisMetastasisLesions
1993
Epidemiology of Cutaneous Leishmaniasis in Colombia: Environmental and Behavioral Risk Factors for Infection, Clinical Manifestations, and Pathogenicity
Weigle K, Santrich C, Martinez F, Valderrama L, Saravia N. Epidemiology of Cutaneous Leishmaniasis in Colombia: Environmental and Behavioral Risk Factors for Infection, Clinical Manifestations, and Pathogenicity. The Journal Of Infectious Diseases 1993, 168: 709-714. PMID: 8354913, DOI: 10.1093/infdis/168.3.709.Peer-Reviewed Original ResearchConceptsRisk factorsFarming occupationLeishmania panamensis infectionRisk factor informationBehavioral risk factorsAcquisition of infectionYears of ageRisk of lesionsPanamensis infectionLeishmanial lesionsClinical manifestationsMale sexActive surveillanceLeishmania infectionCase ascertainmentCutaneous leishmaniasisNew infectionsInfectionLeishmania braziliensisLesionsFactor informationStrongest predictorAgeLeishmaniasisSex