2018
Resistance of Leishmania (Viannia) Panamensis to Meglumine Antimoniate or Miltefosine Modulates Neutrophil Effector Functions
Regli IB, Fernández OL, Martínez-Salazar B, Gómez MA, Saravia NG, Tacchini-Cottier F. Resistance of Leishmania (Viannia) Panamensis to Meglumine Antimoniate or Miltefosine Modulates Neutrophil Effector Functions. Frontiers In Immunology 2018, 9: 3040. PMID: 30622537, PMCID: PMC6308327, DOI: 10.3389/fimmu.2018.03040.Peer-Reviewed Original ResearchConceptsNeutrophil effector functionsMeglumine antimoniateNeutrophil extracellular trapsEffector functionsLeishmania panamensisCell surface activation markersHuman neutrophilsExpression of CD66bReactive oxygen speciesSurface activation markersDrug-susceptible strainsOutcome of infectionMain causative agentChronic lesionsActivation markersDrug-resistant linesNeutrophil activationExtracellular trapsCutaneous leishmaniasisDrug susceptibilityNeutrophilsMurine neutrophilsDecreased expressionMiltefosineNET formation
2017
Profiling gene expression of antimony response genes in Leishmania (Viannia) panamensis and infected macrophages and its relationship with drug susceptibility
Barrera MC, Rojas LJ, Weiss A, Fernandez O, McMahon-Pratt D, Saravia NG, Gomez MA. Profiling gene expression of antimony response genes in Leishmania (Viannia) panamensis and infected macrophages and its relationship with drug susceptibility. Acta Tropica 2017, 176: 355-363. PMID: 28843396, PMCID: PMC5633519, DOI: 10.1016/j.actatropica.2017.08.017.Peer-Reviewed Original ResearchConceptsLeishmania resistanceDrug susceptibilityDrug resistanceHost cell gene expressionClinical strainsCell gene expressionExpression of ABCA2THP-1 cellsPrimary human macrophagesLeishmania susceptibilityGene expressionAntimonial drugsCutaneous leishmaniasisInfected macrophagesLeishmania panamensisIntracellular amastigotesHuman macrophagesResistant strainsSusceptible parasitesAntimonialsIntracellular parasitesLeishmania speciesMacrophagesL. panamensisAQP-9
2014
Chronicity of Dermal Leishmaniasis Caused by Leishmania panamensis Is Associated with Parasite-Mediated Induction of Chemokine Gene Expression
Navas A, Vargas DA, Freudzon M, McMahon-Pratt D, Saravia NG, Gómez MA. Chronicity of Dermal Leishmaniasis Caused by Leishmania panamensis Is Associated with Parasite-Mediated Induction of Chemokine Gene Expression. Infection And Immunity 2014, 82: 2872-2880. PMID: 24752514, PMCID: PMC4097649, DOI: 10.1128/iai.01133-13.Peer-Reviewed Original ResearchConceptsSelf-healed patientsChronic cutaneous leishmaniasisDermal leishmaniasisChemokine gene expressionClinical outcomesBiopsy specimensChronic diseasesInflammatory responseLeishmania panamensisExpression levelsCCR5 receptor geneInduction of CXCL5Montenegro skin testLesion biopsy specimensL. panamensisSelf-healing diseaseNew therapeutic targetsReverse transcription-quantitative PCRTranscription-quantitative PCRChemotactic chemokinesInflammation contributesSkin testAsymptomatic infectionInflammatory activationImmune cells
2013
Treatment Failure and Miltefosine Susceptibility in Dermal Leishmaniasis Caused by Leishmania Subgenus Viannia Species
Obonaga R, Fernández O, Valderrama L, Rubiano L, del Mar Castro M, Barrera M, Gomez M, Saravia N. Treatment Failure and Miltefosine Susceptibility in Dermal Leishmaniasis Caused by Leishmania Subgenus Viannia Species. Antimicrobial Agents And Chemotherapy 2013, 58: 144-152. PMID: 24145529, PMCID: PMC3910710, DOI: 10.1128/aac.01023-13.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultATP Binding Cassette Transporter, Subfamily GATP-Binding Cassette TransportersChildDrug ResistanceFemaleHumansLeishmaniaLeishmaniasis, CutaneousMaleMiddle AgedMultidrug Resistance-Associated Protein 2Multidrug Resistance-Associated ProteinsPhosphorylcholineProspective StudiesTreatment FailureYoung AdultConceptsTreatment failureDermal leishmaniasisQuantitative reverse transcription PCRLoss of susceptibilityMiltefosine susceptibilityDrug susceptibilityIntracellular amastigotesL. panamensis infectionsL. braziliensis infectionPanamensis infectionMucocutaneous diseaseMiltefosine treatmentBraziliensis infectionCutaneous lesionsProspective evaluationMucosal diseaseDrug exposureReverse transcription-PCRClinical failureIndividual patientsGene polymorphismsLeishmania panamensisConcurrent conditionsDecreased expressionTransporter expression
2012
Novel Approach to In Vitro Drug Susceptibility Assessment of Clinical Strains of Leishmania spp
Fernández O, Diaz-Toro Y, Valderrama L, Ovalle C, Valderrama M, Castillo H, Perez M, Saravia NG. Novel Approach to In Vitro Drug Susceptibility Assessment of Clinical Strains of Leishmania spp. Journal Of Clinical Microbiology 2012, 50: 2207-2211. PMID: 22518860, PMCID: PMC3405580, DOI: 10.1128/jcm.00216-12.Peer-Reviewed Original ResearchConceptsClinical strainsMeglumine antimoniateDrug susceptibilityCell ratioParasite burdenAntileishmanial drugsDrug susceptibility assessmentReduction of infectionParasites/cellIntracellular burdenAntimonial drugsLeishmania panamensisDrug concentrationsEffective dosesHuman macrophagesPresence of drugsL. braziliensisParasite growthHost cell ratioMiltefosineLeishmania sppDrugsL. guyanensisLeishmaniaAntimoniateNoninferiority of Miltefosine Versus Meglumine Antimoniate for Cutaneous Leishmaniasis in Children
Rubiano L, Miranda M, Arenas S, Montero L, Rodríguez-Barraquer I, Garcerant D, Prager M, Osorio L, Rojas M, Pérez M, Nicholls R, Saravia N. Noninferiority of Miltefosine Versus Meglumine Antimoniate for Cutaneous Leishmaniasis in Children. The Journal Of Infectious Diseases 2012, 205: 684-692. PMID: 22238470, PMCID: PMC3266136, DOI: 10.1093/infdis/jir816.Peer-Reviewed Original ResearchConceptsPediatric cutaneous leishmaniasisMeglumine antimoniateCutaneous leishmaniasisTreatment failureInitiation of treatmentNoninferiority clinical trialPercent of childrenLow response rateOral miltefosineAdverse eventsMasked evaluationPrimary outcomeTreat analysisWeek 26Clinical trialsOral administrationAntimonial drugsTreatment groupsResponse rateAntimoniateLeishmania panamensisLeishmania guyanensisMiltefosineLeishmaniasisElimination rate
2011
Toll-Like Receptors Participate in Macrophage Activation and Intracellular Control of Leishmania (Viannia) panamensis
Gallego C, Golenbock D, Gomez MA, Saravia NG. Toll-Like Receptors Participate in Macrophage Activation and Intracellular Control of Leishmania (Viannia) panamensis. Infection And Immunity 2011, 79: 2871-2879. PMID: 21518783, PMCID: PMC3191987, DOI: 10.1128/iai.01388-10.Peer-Reviewed Original ResearchConceptsToll-like receptorsEndosomal Toll-like receptorsTNF-α productionMacrophage activationLeishmania panamensisTumor necrosis factor-alpha secretionNecrosis factor-alpha secretionHost macrophagesL. panamensis infectionsLack of TLR2Specific TLR ligandsBone marrow-derived macrophagesIntracellular parasite survivalTNF-α secretionMurine bone marrow-derived macrophagesMarrow-derived macrophagesHuman primary macrophagesEarly time pointsMyD88/Panamensis infectionMouse macrophage cell lineTLR4 expressionTLR ligandsHost cell responsesAlpha secretion
2009
Detection of Leishmania in Unaffected Mucosal Tissues of Patients with Cutaneous Leishmaniasis Caused by Leishmania (Viannia) Species
Figueroa RA, Lozano LE, Romero IC, Cardona MT, Prager M, Pacheco R, Diaz YR, Tellez JA, Saravia NG. Detection of Leishmania in Unaffected Mucosal Tissues of Patients with Cutaneous Leishmaniasis Caused by Leishmania (Viannia) Species. The Journal Of Infectious Diseases 2009, 200: 638-646. PMID: 19569974, PMCID: PMC5056532, DOI: 10.1086/600109.Peer-Reviewed Original ResearchConceptsKinetoplast minicircle DNAMucosal tissuesCutaneous leishmaniasisMucocutaneous leishmaniasisNasal mucosaSwab samplesMucosa of patientsLeishmania speciesPresence of LeishmaniaDetection of LeishmaniaMucosal leishmaniasisMucosal diseaseCutaneous diseaseLeishmania infectionPolymerase chain reactionParasitological evaluationPatientsLeishmania panamensisLeishmania guyanensisMucosaNatural historyLeishmaniasisLeishmania braziliensisChain reactionAsymptomatic presence
2008
Antimony Resistance and Trypanothione in Experimentally Selected and Clinical Strains of Leishmania panamensis
Goyeneche-Patino DA, Valderrama L, Walker J, Saravia NG. Antimony Resistance and Trypanothione in Experimentally Selected and Clinical Strains of Leishmania panamensis. Antimicrobial Agents And Chemotherapy 2008, 52: 4503-4506. PMID: 18824610, PMCID: PMC2592887, DOI: 10.1128/aac.01075-08.Peer-Reviewed Original Research
2004
Efficacy and toxicity of pentavalent antimonials (Glucantime and Pentostam) in an American cutaneous leishmaniasis animal model: luminometry application.
Henao HH, Osorio Y, Saravia NG, Gómez A, Travi B. Efficacy and toxicity of pentavalent antimonials (Glucantime and Pentostam) in an American cutaneous leishmaniasis animal model: luminometry application. Biomédica 2004, 24: 393-402. PMID: 15678803, DOI: 10.7705/biomedica.v24i4.1289.Peer-Reviewed Original ResearchConceptsAnti-Leishmania treatmentNormal serum levelsFirst-line drugsRight hind footSite of injectionParasitological efficacySerum levelsClinical efficacyLesion reductionCure rateLine drugsPentavalent antimonialsSimilar efficacyHepatic alterationsAlanine aminotransferaseLocal toxicityClinical observationsMicroscopic signsTreatment efficacyAnimal modelsHigh dosesLeishmania panamensisAspartate aminotransferaseParasite burdenGlucantime
2000
INTRASPECIFIC HETEROGENEITY IN THE MINI-EXON GENE LOCALIZATION OF LEISHMANIA (VIANNIA) PANAMENSIS AND LEISHMANIA (VIANNIA) GUYANENSIS FROM COLOMBIA
Pacheco R, Fernandes O, Salinas G, Segura I, Momen H, Degrave W, Saravia N, Campbell D. INTRASPECIFIC HETEROGENEITY IN THE MINI-EXON GENE LOCALIZATION OF LEISHMANIA (VIANNIA) PANAMENSIS AND LEISHMANIA (VIANNIA) GUYANENSIS FROM COLOMBIA. Journal Of Parasitology 2000, 86: 1250-1253. PMID: 11191900, DOI: 10.1645/0022-3395(2000)086[1250:ihitme]2.0.co;2.Peer-Reviewed Original ResearchConceptsMini-exon gene sequencesGene sequencesGene localizationMini-exon geneChromosomal localizationGenetic variabilityL. guyanensisIntraspecific heterogeneityDifferent karyotypesChromosomesPanamensisKbL. panamensisSpeciesLeishmania panamensisMolecular epidemiologic markerViannia subgenusHuman isolates
1998
Permissiveness of human monocytes and monocyte-derived macrophages to infection by promastigotes of Leishmania (Viannia) panamensis.
Bosque F, Milon G, Valderrama L, Saravia N. Permissiveness of human monocytes and monocyte-derived macrophages to infection by promastigotes of Leishmania (Viannia) panamensis. Journal Of Parasitology 1998, 84: 1250-6. PMID: 9920322, DOI: 10.2307/3284682.Peer-Reviewed Original ResearchConceptsPercentage of cellsBlood monocytesInduction of differentiationLeishmania panamensis infectionHuman peripheral blood monocytesHistiocytic cell line UDifferentiated macrophagesExpression of CD68Number of amastigotesPeripheral blood monocytesOutcome of infectionSite of infectionMonocyte-derived macrophagesL. panamensis promastigotesMononuclear phagocytic cellsPhorbol myristate acetatePanamensis infectionNumber of parasitesCell line UBone marrowFresh monocytesLeishmania panamensisMonocytesResident macrophagesStages of differentiation
1997
In Situ Characterization of the Human Host Response to Leishmania panamensis
Palma G, Saravia N. In Situ Characterization of the Human Host Response to Leishmania panamensis. American Journal Of Dermatopathology 1997, 19: 585-590. PMID: 9415615, DOI: 10.1097/00000372-199712000-00006.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAnimalsAntibodies, ProtozoanAntigen-Antibody ReactionsAntigens, ProtozoanBiomarkersBiopsyCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesEpidermisFluorescent Antibody Technique, IndirectGranulocytesGranulomaHumansImmunity, CellularImmunohistochemistryLeishmaniaLeishmaniasisLymphocyte ActivationMaleMiddle AgedNecrosisRegression AnalysisSkinConceptsHuman host responseHost responseLeishmania panamensisPresence of amastigotesT cell subpopulationsImmune response dataIndirect immunoperoxidase methodCD8 ratioDermal infiltrateMale patientsHistopathologic changesParticular cell populationPrognostic indicatorLeishmania infectionSkin biopsiesPlasma cellsT cellsDermal distributionCutaneous leishmaniasisProspective analysisB cellsB lymphocytesUpper dermisImmunoperoxidase methodDisease evolution
1996
The inflammatory response promotes cutaneous metastasis in hamsters infected with Leishmania (Viannia) panamensis.
Travi B, Osorio Y, Saravia N. The inflammatory response promotes cutaneous metastasis in hamsters infected with Leishmania (Viannia) panamensis. Journal Of Parasitology 1996, 82: 454-7. PMID: 8636852, DOI: 10.2307/3284085.Peer-Reviewed Original ResearchConceptsInflammatory responseMetastatic lesionsControl animalsL. panamensisLeishmania panamensis infectionType hypersensitivity responseFrequency of metastasisNonspecific inflammatory responseDevelopment of lesionsExperimental groupElicitation of inflammationPanamensis infectionSkin metastasesCutaneous metastasesHind pawsSurgical excisionHypersensitivity responseSite of inductionIntradermal applicationSecondary diseaseHamster modelInflammatory stimuliLeishmania panamensisMetastasisLesions
1994
Human monocyte infection by Leishmania (Viannia) panamensis. Role of complement receptors and correlation of susceptibility in vitro with clinical phenotype.
Robledo S, Wozencraft A, Valencia A, Saravia N. Human monocyte infection by Leishmania (Viannia) panamensis. Role of complement receptors and correlation of susceptibility in vitro with clinical phenotype. The Journal Of Immunology 1994, 152: 1265-76. PMID: 8301130, DOI: 10.4049/jimmunol.152.3.1265.Peer-Reviewed Original ResearchConceptsPeripheral blood monocytesClinical phenotypeHuman monocytesDistinctive clinical outcomesDifferent clinical groupsComplement receptors CR1Human monocyte cell line UPercentage of cellsPatterns of susceptibilityMonocyte infectionPanamensis infectionClinical outcomesDisease 5Number of parasitesStationary phase promastigotesBlood monocytesComplement receptorsCell line UHealthy individualsReceptors CR1Leishmania panamensisExperimental infectionInfectionUnexposed controlsAntireceptor antibodies
1991
Metastatic capability of Leishmania (Viannia) panamensis and Leishmania (Viannia) guyanensis in golden hamsters.
Martinez J, Travi B, Valencia A, Saravia N. Metastatic capability of Leishmania (Viannia) panamensis and Leishmania (Viannia) guyanensis in golden hamsters. Journal Of Parasitology 1991, 77: 762-8. PMID: 1919926, DOI: 10.2307/3282713.Peer-Reviewed Original ResearchConceptsCutaneous metastatic lesionsMetastatic lesionsFrequency of isolationLymph nodesL. panamensisL. guyanensisLeishmania panamensisLeishmania guyanensisGolden hamstersDistant lymph nodesCutaneous metastasesPrimary lesionLymphatic disseminationExperimental infectionLesionsMetastatic capabilityHuman casesTissue samplesHamstersParasite culturesSandfly vectorNineteen strainsInoculation siteGuyanensisInoculum dose