2012
Novel Approach to In Vitro Drug Susceptibility Assessment of Clinical Strains of Leishmania spp
Fernández O, Diaz-Toro Y, Valderrama L, Ovalle C, Valderrama M, Castillo H, Perez M, Saravia NG. Novel Approach to In Vitro Drug Susceptibility Assessment of Clinical Strains of Leishmania spp. Journal Of Clinical Microbiology 2012, 50: 2207-2211. PMID: 22518860, PMCID: PMC3405580, DOI: 10.1128/jcm.00216-12.Peer-Reviewed Original ResearchConceptsClinical strainsMeglumine antimoniateDrug susceptibilityCell ratioParasite burdenAntileishmanial drugsDrug susceptibility assessmentReduction of infectionParasites/cellIntracellular burdenAntimonial drugsLeishmania panamensisDrug concentrationsEffective dosesHuman macrophagesPresence of drugsL. braziliensisParasite growthHost cell ratioMiltefosineLeishmania sppDrugsL. guyanensisLeishmaniaAntimoniate
2005
Role of the ABC Transporter MRPA (PGPA) in Antimony Resistance in Leishmania infantum Axenic and Intracellular Amastigotes
Fadili K, Messier N, Leprohon P, Roy G, Guimond C, Trudel N, Saravia NG, Papadopoulou B, Légaré D, Ouellette M. Role of the ABC Transporter MRPA (PGPA) in Antimony Resistance in Leishmania infantum Axenic and Intracellular Amastigotes. Antimicrobial Agents And Chemotherapy 2005, 49: 1988-1993. PMID: 15855523, PMCID: PMC1087671, DOI: 10.1128/aac.49.5.1988-1993.2005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntimonyAntimony Sodium GluconateATP-Binding Cassette TransportersCell LineCysteineDNA, ProtozoanDrug ResistanceGlutathioneLeishmania infantumMembrane GlycoproteinsMicroscopy, ConfocalMonocytesOligonucleotide Array Sequence AnalysisProtozoan ProteinsRibonucleoproteinsSaccharomyces cerevisiae ProteinsSpermidineTransfectionConceptsABC transporter MRPAAntimony resistanceSodium stibogluconate (SSG) resistanceTreatment of infectionsMRPA geneSodium stibogluconateAntimonial compoundsMain drugsAmastigote parasitesPutative alterationsIntracellular amastigotesLevel of expressionProtozoan parasite LeishmaniaIntracellular parasitesAxenic amastigotesButhionine sulfoximineLevels of cysteineAmastigote stageDNA microarraysS-adenosylhomocysteine hydrolaseParasite LeishmaniaPotassium antimonyl tartrateAmastigotesLeishmaniaGenes
1998
Sensitivity of Leishmania viannia panamensis to Pentavalent Antimony Is Correlated with the Formation of Cleavable DNA-Protein Complexes
Lucumi A, Robledo S, Gama V, Saravia N. Sensitivity of Leishmania viannia panamensis to Pentavalent Antimony Is Correlated with the Formation of Cleavable DNA-Protein Complexes. Antimicrobial Agents And Chemotherapy 1998, 42: 1990-1995. PMID: 9687395, PMCID: PMC105721, DOI: 10.1128/aac.42.8.1990.Peer-Reviewed Original ResearchConceptsSbv/Sodium stibogluconateAntimonial drugsHuman promonocytic cell line UCleavable complexLeishmania Viannia panamensisAntimonial agentsMedian ED50Meglumine antimoniateCell line UEffective doseEffects of camptothecinPentavalent antimonyPromastigotes of LeishmaniaReports of inhibitionInhibitors of topoisomeraseInhibition of topoisomeraseLeishmania donovaniL. panamensisLeishmaniaAntimoniatePatientsRelapseStibogluconateLine U
1996
Leishmania RNA viruses in Leishmania of the Viannia subgenus.
Salinas G, Zamora M, Stuart K, Saravia N. Leishmania RNA viruses in Leishmania of the Viannia subgenus. American Journal Of Tropical Medicine And Hygiene 1996, 54: 425-9. PMID: 8615459, DOI: 10.4269/ajtmh.1996.54.425.Peer-Reviewed Original ResearchConceptsL. braziliensisL. braziliensis strainWorld Health Organization referenceViannia subgenusLeishmania RNA virusCapsid protein fragmentMucosal lesionsStrains of LeishmaniaEpidemiologic markersMetastatic traitsVirus presentKaryotype analysisMaterial reactiveColombian strainL. guyanensisVirusBraziliensisLeishmaniaLRV1Polyclonal antibodiesRNA virusesRecombinant fragmentsTotal lysatesReference strainsBiological characterization