2021
Potency and Preclinical Evidence of Synergy of Oral Azole Drugs and Miltefosine in an Ex Vivo Model of Leishmania (Viannia) panamensis Infection
Fernández OL, Rosales-Chilama M, Quintero N, Travi BL, Wetzel DM, Gómez MA, Saravia NG. Potency and Preclinical Evidence of Synergy of Oral Azole Drugs and Miltefosine in an Ex Vivo Model of Leishmania (Viannia) panamensis Infection. Antimicrobial Agents And Chemotherapy 2021, 66: e01425-21. PMID: 34694879, PMCID: PMC8765415, DOI: 10.1128/aac.01425-21.Peer-Reviewed Original ResearchConceptsFractional inhibitory concentration indexPeripheral blood mononuclear cellsL. panamensisHuman peripheral blood mononuclear cellsClinical strainsLeishmania panamensis infectionOral azole drugsOral combination therapyFailure of treatmentBlood mononuclear cellsEfficacy of treatmentNovel therapeutic strategiesEffectiveness of treatmentHigh potencyReduction of infectionEx vivo modelInhibitory concentration indexFree drug concentrationPanamensis infectionPreclinical evidenceCombination therapyMononuclear cellsAntimonial drugsCutaneous leishmaniasisDrug combinations
2017
Profiling gene expression of antimony response genes in Leishmania (Viannia) panamensis and infected macrophages and its relationship with drug susceptibility
Barrera MC, Rojas LJ, Weiss A, Fernandez O, McMahon-Pratt D, Saravia NG, Gomez MA. Profiling gene expression of antimony response genes in Leishmania (Viannia) panamensis and infected macrophages and its relationship with drug susceptibility. Acta Tropica 2017, 176: 355-363. PMID: 28843396, PMCID: PMC5633519, DOI: 10.1016/j.actatropica.2017.08.017.Peer-Reviewed Original ResearchConceptsLeishmania resistanceDrug susceptibilityDrug resistanceHost cell gene expressionClinical strainsCell gene expressionExpression of ABCA2THP-1 cellsPrimary human macrophagesLeishmania susceptibilityGene expressionAntimonial drugsCutaneous leishmaniasisInfected macrophagesLeishmania panamensisIntracellular amastigotesHuman macrophagesResistant strainsSusceptible parasitesAntimonialsIntracellular parasitesLeishmania speciesMacrophagesL. panamensisAQP-9
2016
An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections
Saldarriaga OA, Castellanos-Gonzalez A, Porrozzi R, Baldeviano GC, Lescano AG, de Los Santos MB, Fernandez OL, Saravia NG, Costa E, Melby PC, Travi BL. An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections. PLOS Neglected Tropical Diseases 2016, 10: e0004638. PMID: 27115155, PMCID: PMC4845993, DOI: 10.1371/journal.pntd.0004638.Peer-Reviewed Original ResearchConceptsMucosal leishmaniasisDiagnostic testsL. braziliensisPrimary health systemL. panamensisRPA-LFSensitive diagnostic testMost endemic regionsMolecular diagnostic testsChronic lesionsReal-time PCRCutaneous leishmaniasisEndemic regionsParasite burdenHealth systemClinical validationLeishmaniasisL. naiffiMolecular testsParasite DNALeishmania sppTime PCRClinical samplesViannia subgenusRecombinase polymerase amplification
2014
Miltefosine and Antimonial Drug Susceptibility of Leishmania Viannia Species and Populations in Regions of High Transmission in Colombia
Fernández OL, Diaz-Toro Y, Ovalle C, Valderrama L, Muvdi S, Rodríguez I, Gomez MA, Saravia NG. Miltefosine and Antimonial Drug Susceptibility of Leishmania Viannia Species and Populations in Regions of High Transmission in Colombia. PLOS Neglected Tropical Diseases 2014, 8: e2871. PMID: 24853871, PMCID: PMC4031164, DOI: 10.1371/journal.pntd.0002871.Peer-Reviewed Original ResearchConceptsV. panamensisLine treatmentClinical strainsDrug susceptibilitySecond-line treatmentFirst-line treatmentEmergence of resistancePopulations of LeishmaniaViannia speciesResistant clinical strainsAntimony susceptibilityClinical evidenceMeglumine antimoniatePentavalent antimonialsDermal leishmaniasisEpidemiologic differencesLeishmania VianniaProxy markerIntracellular amastigotesMunicipality of TumacoResistant strainsMiltefosineDisparate susceptibilityDrugsL. panamensisChronicity of Dermal Leishmaniasis Caused by Leishmania panamensis Is Associated with Parasite-Mediated Induction of Chemokine Gene Expression
Navas A, Vargas DA, Freudzon M, McMahon-Pratt D, Saravia NG, Gómez MA. Chronicity of Dermal Leishmaniasis Caused by Leishmania panamensis Is Associated with Parasite-Mediated Induction of Chemokine Gene Expression. Infection And Immunity 2014, 82: 2872-2880. PMID: 24752514, PMCID: PMC4097649, DOI: 10.1128/iai.01133-13.Peer-Reviewed Original ResearchConceptsSelf-healed patientsChronic cutaneous leishmaniasisDermal leishmaniasisChemokine gene expressionClinical outcomesBiopsy specimensChronic diseasesInflammatory responseLeishmania panamensisExpression levelsCCR5 receptor geneInduction of CXCL5Montenegro skin testLesion biopsy specimensL. panamensisSelf-healing diseaseNew therapeutic targetsReverse transcription-quantitative PCRTranscription-quantitative PCRChemotactic chemokinesInflammation contributesSkin testAsymptomatic infectionInflammatory activationImmune cells
2012
Human Macrophage Response to L. (Viannia) panamensis: Microarray Evidence for an Early Inflammatory Response
Ramírez C, Díaz-Toro Y, Tellez J, Castilho TM, Rojas R, Ettinger NA, Tikhonova I, Alexander ND, Valderrama L, Hager J, Wilson ME, Lin A, Zhao H, Saravia NG, McMahon-Pratt D. Human Macrophage Response to L. (Viannia) panamensis: Microarray Evidence for an Early Inflammatory Response. PLOS Neglected Tropical Diseases 2012, 6: e1866. PMID: 23145196, PMCID: PMC3493378, DOI: 10.1371/journal.pntd.0001866.Peer-Reviewed Original ResearchConceptsMRNA abundance profilesMonocyte-derived macrophagesRegulation of genesDistinct biologic responsesAbundance profilesL. panamensisDifferential gene expressionEarly time pointsMacrophage responseEarly macrophage responseImmune responseLeishmania speciesHuman monocyte-derived macrophagesMicroarray evidenceOutset of infectionGene regulationActivation of PKCHuman macrophage responseGene expressionLeishmania panamensis infectionTime pointsCellular responsesAdaptive immune responsesG proteinsEarly inflammatory response
2009
Role of Toll-like receptors in the response of human macrophages to Leishmania panamensis infection (133.17)
Gallego C, Saravia N, Valderrama L, Golenbock D. Role of Toll-like receptors in the response of human macrophages to Leishmania panamensis infection (133.17). The Journal Of Immunology 2009, 182: 133.17-133.17. DOI: 10.4049/jimmunol.182.supp.133.17.Peer-Reviewed Original ResearchToll-like receptorsOutcome of infectionPanamensis infectionHuman macrophagesTLR ligandsL. panamensisExpression of TLR1L. panamensis infectionsLeishmania panamensis infectionActivation of TLR2Production of TNFαAmerican cutaneous leishmaniasisHuman primary macrophagesL. panamensis promastigotesPossible roleHealthy donorsCutaneous leishmaniasisTNFα productionInnate recognitionWild-type (WT) mouse macrophagesInfectionPrimary macrophagesTLR2MacrophagesPhagocytic cells
2008
Etiologic agent of an epidemic of cutaneous leishmaniasis in Tolima, Colombia.
Rodríguez-Barraquer I, Góngora R, Prager M, Pacheco R, Montero LM, Navas A, Ferro C, Miranda MC, Saravia NG. Etiologic agent of an epidemic of cutaneous leishmaniasis in Tolima, Colombia. American Journal Of Tropical Medicine And Hygiene 2008, 78: 276-82. PMID: 18256429, DOI: 10.4269/ajtmh.2008.78.276.Peer-Reviewed Original ResearchConceptsAmerican cutaneous leishmaniasisCutaneous leishmaniasisEtiologic agentLeishmania guyanensisProbable etiologic agentLeishmaniasis control programLarge epidemicsMonoclonal antibodiesLeishmania isolatesLeishmaniasisPeridomestic settingsZoonotic diseaseL. panamensisEpidemicDomestic transmissionControl programsPresent studyIsoenzyme electrophoresisIsolatesAgentsGuyanensisPatientsDiseaseDiagnosisAntibodies
2006
Resistance to Oxidative Stress Is Associated with Metastasis in Mucocutaneous Leishmaniasis
Acestor N, Masina S, Ives A, Walker J, Saravia NG, Fasel N. Resistance to Oxidative Stress Is Associated with Metastasis in Mucocutaneous Leishmaniasis. The Journal Of Infectious Diseases 2006, 194: 1160-1167. PMID: 16991092, DOI: 10.1086/507646.Peer-Reviewed Original ResearchConceptsMucocutaneous leishmaniasisMetastatic phenotypeMetastatic diseaseCutaneous lesionsMicrobicidal responsesMetastatic disseminationGolden hamstersNasopharyngeal tissuesCytoplasmic peroxiredoxinOxidative stressLeishmania promastigotesNonmetastatic clonesL. panamensisLeishmaniasisHost cellsParasitesPhenotypePatientsMetastasisHeat shockPathogenesisLesionsPeroxidase activityDiseaseDissemination
2000
INTRASPECIFIC HETEROGENEITY IN THE MINI-EXON GENE LOCALIZATION OF LEISHMANIA (VIANNIA) PANAMENSIS AND LEISHMANIA (VIANNIA) GUYANENSIS FROM COLOMBIA
Pacheco R, Fernandes O, Salinas G, Segura I, Momen H, Degrave W, Saravia N, Campbell D. INTRASPECIFIC HETEROGENEITY IN THE MINI-EXON GENE LOCALIZATION OF LEISHMANIA (VIANNIA) PANAMENSIS AND LEISHMANIA (VIANNIA) GUYANENSIS FROM COLOMBIA. Journal Of Parasitology 2000, 86: 1250-1253. PMID: 11191900, DOI: 10.1645/0022-3395(2000)086[1250:ihitme]2.0.co;2.Peer-Reviewed Original ResearchConceptsMini-exon gene sequencesGene sequencesGene localizationMini-exon geneChromosomal localizationGenetic variabilityL. guyanensisIntraspecific heterogeneityDifferent karyotypesChromosomesPanamensisKbL. panamensisSpeciesLeishmania panamensisMolecular epidemiologic markerViannia subgenusHuman isolates
1998
Sensitivity of Leishmania viannia panamensis to Pentavalent Antimony Is Correlated with the Formation of Cleavable DNA-Protein Complexes
Lucumi A, Robledo S, Gama V, Saravia N. Sensitivity of Leishmania viannia panamensis to Pentavalent Antimony Is Correlated with the Formation of Cleavable DNA-Protein Complexes. Antimicrobial Agents And Chemotherapy 1998, 42: 1990-1995. PMID: 9687395, PMCID: PMC105721, DOI: 10.1128/aac.42.8.1990.Peer-Reviewed Original ResearchConceptsSbv/Sodium stibogluconateAntimonial drugsHuman promonocytic cell line UCleavable complexLeishmania Viannia panamensisAntimonial agentsMedian ED50Meglumine antimoniateCell line UEffective doseEffects of camptothecinPentavalent antimonyPromastigotes of LeishmaniaReports of inhibitionInhibitors of topoisomeraseInhibition of topoisomeraseLeishmania donovaniL. panamensisLeishmaniaAntimoniatePatientsRelapseStibogluconateLine U
1997
Carbohydrate and LPG expression in Leishmania viannia subgenus.
Muskus C, Segura I, Oddone R, Turco S, Leiby D, Toro L, Robledo S, Saravia N. Carbohydrate and LPG expression in Leishmania viannia subgenus. Journal Of Parasitology 1997, 83: 671-8. PMID: 9267410, DOI: 10.2307/3284245.Peer-Reviewed Original ResearchConceptsGrowth phaseLogarithmic phase promastigotesRepeat unitsViannia subgenusLPG repeat unitsSpecies referenceL. donovani lipophosphoglycanLPG expressionMolecular determinantsSubgeneraCell surfaceGlycosylated moleculesStationary phase promastigotesL. donovaniDistinct populationsBiochemical analysisExpression of carbohydratesSpeciesExpressionL. panamensisL. guyanensisSuch moleculesL. braziliensisLeishmania promastigotesFlow cytometry
1996
The inflammatory response promotes cutaneous metastasis in hamsters infected with Leishmania (Viannia) panamensis.
Travi B, Osorio Y, Saravia N. The inflammatory response promotes cutaneous metastasis in hamsters infected with Leishmania (Viannia) panamensis. Journal Of Parasitology 1996, 82: 454-7. PMID: 8636852, DOI: 10.2307/3284085.Peer-Reviewed Original ResearchConceptsInflammatory responseMetastatic lesionsControl animalsL. panamensisLeishmania panamensis infectionType hypersensitivity responseFrequency of metastasisNonspecific inflammatory responseDevelopment of lesionsExperimental groupElicitation of inflammationPanamensis infectionSkin metastasesCutaneous metastasesHind pawsSurgical excisionHypersensitivity responseSite of inductionIntradermal applicationSecondary diseaseHamster modelInflammatory stimuliLeishmania panamensisMetastasisLesions
1991
Metastatic capability of Leishmania (Viannia) panamensis and Leishmania (Viannia) guyanensis in golden hamsters.
Martinez J, Travi B, Valencia A, Saravia N. Metastatic capability of Leishmania (Viannia) panamensis and Leishmania (Viannia) guyanensis in golden hamsters. Journal Of Parasitology 1991, 77: 762-8. PMID: 1919926, DOI: 10.2307/3282713.Peer-Reviewed Original ResearchConceptsCutaneous metastatic lesionsMetastatic lesionsFrequency of isolationLymph nodesL. panamensisL. guyanensisLeishmania panamensisLeishmania guyanensisGolden hamstersDistant lymph nodesCutaneous metastasesPrimary lesionLymphatic disseminationExperimental infectionLesionsMetastatic capabilityHuman casesTissue samplesHamstersParasite culturesSandfly vectorNineteen strainsInoculation siteGuyanensisInoculum dose