2016
An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections
Saldarriaga OA, Castellanos-Gonzalez A, Porrozzi R, Baldeviano GC, Lescano AG, de Los Santos MB, Fernandez OL, Saravia NG, Costa E, Melby PC, Travi BL. An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections. PLOS Neglected Tropical Diseases 2016, 10: e0004638. PMID: 27115155, PMCID: PMC4845993, DOI: 10.1371/journal.pntd.0004638.Peer-Reviewed Original ResearchConceptsMucosal leishmaniasisDiagnostic testsL. braziliensisPrimary health systemL. panamensisRPA-LFSensitive diagnostic testMost endemic regionsMolecular diagnostic testsChronic lesionsReal-time PCRCutaneous leishmaniasisEndemic regionsParasite burdenHealth systemClinical validationLeishmaniasisL. naiffiMolecular testsParasite DNALeishmania sppTime PCRClinical samplesViannia subgenusRecombinase polymerase amplification
2012
Novel Approach to In Vitro Drug Susceptibility Assessment of Clinical Strains of Leishmania spp
Fernández O, Diaz-Toro Y, Valderrama L, Ovalle C, Valderrama M, Castillo H, Perez M, Saravia NG. Novel Approach to In Vitro Drug Susceptibility Assessment of Clinical Strains of Leishmania spp. Journal Of Clinical Microbiology 2012, 50: 2207-2211. PMID: 22518860, PMCID: PMC3405580, DOI: 10.1128/jcm.00216-12.Peer-Reviewed Original ResearchConceptsClinical strainsMeglumine antimoniateDrug susceptibilityCell ratioParasite burdenAntileishmanial drugsDrug susceptibility assessmentReduction of infectionParasites/cellIntracellular burdenAntimonial drugsLeishmania panamensisDrug concentrationsEffective dosesHuman macrophagesPresence of drugsL. braziliensisParasite growthHost cell ratioMiltefosineLeishmania sppDrugsL. guyanensisLeishmaniaAntimoniate
2011
Leishmania (Viannia) Infection in the Domestic Dog in Chaparral, Colombia
Santaella J, Ocampo CB, Saravia NG, Méndez F, Góngora R, Gomez MA, Munstermann LE, Quinnell RJ. Leishmania (Viannia) Infection in the Domestic Dog in Chaparral, Colombia. American Journal Of Tropical Medicine And Hygiene 2011, 84: 674-680. PMID: 21540374, PMCID: PMC3083732, DOI: 10.4269/ajtmh.2011.10-0159.Peer-Reviewed Original ResearchConceptsCutaneous leishmaniasisSkin biopsy specimensHuman cutaneous leishmaniasisReservoirs of LeishmaniaEnzyme-linked immunosorbentAmerican cutaneous leishmaniasisPrevalence of infectionBiopsy specimensLeishmania infectionRisk factorsCanine infectionLeishmania guyanensisBuffy coatL. braziliensisDogsInfectionLeishmaniasisPeridomestic transmissionL. guyanensisPositive resultsBlottingMore testsDomestic dogsGuyanensisBiopsy
1997
Carbohydrate and LPG expression in Leishmania viannia subgenus.
Muskus C, Segura I, Oddone R, Turco S, Leiby D, Toro L, Robledo S, Saravia N. Carbohydrate and LPG expression in Leishmania viannia subgenus. Journal Of Parasitology 1997, 83: 671-8. PMID: 9267410, DOI: 10.2307/3284245.Peer-Reviewed Original ResearchConceptsGrowth phaseLogarithmic phase promastigotesRepeat unitsViannia subgenusLPG repeat unitsSpecies referenceL. donovani lipophosphoglycanLPG expressionMolecular determinantsSubgeneraCell surfaceGlycosylated moleculesStationary phase promastigotesL. donovaniDistinct populationsBiochemical analysisExpression of carbohydratesSpeciesExpressionL. panamensisL. guyanensisSuch moleculesL. braziliensisLeishmania promastigotesFlow cytometry
1996
Leishmania RNA viruses in Leishmania of the Viannia subgenus.
Salinas G, Zamora M, Stuart K, Saravia N. Leishmania RNA viruses in Leishmania of the Viannia subgenus. American Journal Of Tropical Medicine And Hygiene 1996, 54: 425-9. PMID: 8615459, DOI: 10.4269/ajtmh.1996.54.425.Peer-Reviewed Original ResearchConceptsL. braziliensisL. braziliensis strainWorld Health Organization referenceViannia subgenusLeishmania RNA virusCapsid protein fragmentMucosal lesionsStrains of LeishmaniaEpidemiologic markersMetastatic traitsVirus presentKaryotype analysisMaterial reactiveColombian strainL. guyanensisVirusBraziliensisLeishmaniaLRV1Polyclonal antibodiesRNA virusesRecombinant fragmentsTotal lysatesReference strainsBiological characterization
1992
Haemoculture of Leishmania (Viannia) braziliensis from two cases of mucosal leishmaniasis: Re-examination of haematogenous dissemination
Martinez J, Alba, Arias L, Escobar M, Saravia N. Haemoculture of Leishmania (Viannia) braziliensis from two cases of mucosal leishmaniasis: Re-examination of haematogenous dissemination. Transactions Of The Royal Society Of Tropical Medicine And Hygiene 1992, 86: 392-394. PMID: 1440813, DOI: 10.1016/0035-9203(92)90233-3.Peer-Reviewed Original ResearchConceptsMucosal leishmaniasisActive pulmonary tuberculosisMontenegro skin testPeripheral blood leucocytesIsolation of parasitesIndirect fluorescent antibodyCases of leishmaniasisCulture of parasitesHaematogenous disseminationMucosal involvementPulmonary tuberculosisCutaneous lesionsSkin testMucosal diseaseMetastatic spreadMonths durationBlood leucocytesRight legPatientsLeishmania parasitesLeishmania braziliensisL. braziliensisLeishmaniasisFluorescent antibodyLeucocytes