2018
Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Assay for Diagnosis of Cutaneous and Visceral Leishmaniasis
Adams ER, Schoone G, Versteeg I, Gomez MA, Diro E, Mori Y, Perlee D, Downing T, Saravia N, Assaye A, Hailu A, Albertini A, Ndung'u JM, Schallig H. Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Assay for Diagnosis of Cutaneous and Visceral Leishmaniasis. Journal Of Clinical Microbiology 2018, 56: 10.1128/jcm.00386-18. PMID: 29695527, PMCID: PMC6018344, DOI: 10.1128/jcm.00386-18.Peer-Reviewed Original ResearchConceptsVisceral leishmaniasisLAMP assayDiagnostic accuracyProspective cohort trialDiagnosis of CLBone marrow aspirateMinicircle kinetoplast DNANear-patient settingIsothermal Amplification AssayCohort trialVL patientsCL diagnosisVL suspectsMarrow aspiratesMalaria parasitesSwab samplesDiagnosisWhole bloodGold standardInvasive samplesAnalytical specificityClinical samplesAmplification assaysPatientsReference standard
2016
An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections
Saldarriaga OA, Castellanos-Gonzalez A, Porrozzi R, Baldeviano GC, Lescano AG, de Los Santos MB, Fernandez OL, Saravia NG, Costa E, Melby PC, Travi BL. An Innovative Field-Applicable Molecular Test to Diagnose Cutaneous Leishmania Viannia spp. Infections. PLOS Neglected Tropical Diseases 2016, 10: e0004638. PMID: 27115155, PMCID: PMC4845993, DOI: 10.1371/journal.pntd.0004638.Peer-Reviewed Original ResearchConceptsMucosal leishmaniasisDiagnostic testsL. braziliensisPrimary health systemL. panamensisRPA-LFSensitive diagnostic testMost endemic regionsMolecular diagnostic testsChronic lesionsReal-time PCRCutaneous leishmaniasisEndemic regionsParasite burdenHealth systemClinical validationLeishmaniasisL. naiffiMolecular testsParasite DNALeishmania sppTime PCRClinical samplesViannia subgenusRecombinase polymerase amplification