2009
Detection of Leishmania in Unaffected Mucosal Tissues of Patients with Cutaneous Leishmaniasis Caused by Leishmania (Viannia) Species
Figueroa RA, Lozano LE, Romero IC, Cardona MT, Prager M, Pacheco R, Diaz YR, Tellez JA, Saravia NG. Detection of Leishmania in Unaffected Mucosal Tissues of Patients with Cutaneous Leishmaniasis Caused by Leishmania (Viannia) Species. The Journal Of Infectious Diseases 2009, 200: 638-646. PMID: 19569974, PMCID: PMC5056532, DOI: 10.1086/600109.Peer-Reviewed Original ResearchConceptsKinetoplast minicircle DNAMucosal tissuesCutaneous leishmaniasisMucocutaneous leishmaniasisNasal mucosaSwab samplesMucosa of patientsLeishmania speciesPresence of LeishmaniaDetection of LeishmaniaMucosal leishmaniasisMucosal diseaseCutaneous diseaseLeishmania infectionPolymerase chain reactionParasitological evaluationPatientsLeishmania panamensisLeishmania guyanensisMucosaNatural historyLeishmaniasisLeishmania braziliensisChain reactionAsymptomatic presence
2000
Evaluation of Polymerase Chain Reaction, Adenosine Deaminase, and Interferon-γ in Pleural Fluid for the Differential Diagnosis of Pleural Tuberculosis
Villegas M, Labrada L, Saravia N. Evaluation of Polymerase Chain Reaction, Adenosine Deaminase, and Interferon-γ in Pleural Fluid for the Differential Diagnosis of Pleural Tuberculosis. CHEST Journal 2000, 118: 1355-1364. PMID: 11083686, DOI: 10.1378/chest.118.5.1355.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine DeaminaseAge FactorsBacteriological TechniquesBayes TheoremBiopsyBloodChi-Square DistributionDiagnosis, DifferentialDNA, BacterialFemaleFeverHumansInterferon-gammaMaleMiddle AgedMycobacterium tuberculosisPleural DiseasesPleural EffusionPleural NeoplasmsPolymerase Chain ReactionPredictive Value of TestsSensitivity and SpecificitySputumTuberculosis, PleuralConceptsIFN-gamma levelsADA activity determinationPleural tuberculosisPolymerase chain reactionPleural effusionPleural fluidADA activityNonspecific clinical presentationPleural biopsy specimensTuberculous pleural effusionChain reactionAlternative diagnostic strategiesDifferent clinical settingsNegative predictive valueMeasurement of interferonAdenosine deaminase activityCombination of PCRMalignant etiologyUndetermined etiologyClinical presentationPaucibacillary natureClinical variablesPatient populationPleural biopsyDiagnostic challenge