2015
Parasitological Confirmation and Analysis of Leishmania Diversity in Asymptomatic and Subclinical Infection following Resolution of Cutaneous Leishmaniasis
Rosales-Chilama M, Gongora RE, Valderrama L, Jojoa J, Alexander N, Rubiano LC, Cossio A, Adams ER, Saravia NG, Gomez MA. Parasitological Confirmation and Analysis of Leishmania Diversity in Asymptomatic and Subclinical Infection following Resolution of Cutaneous Leishmaniasis. PLOS Neglected Tropical Diseases 2015, 9: e0004273. PMID: 26659114, PMCID: PMC4684356, DOI: 10.1371/journal.pntd.0004273.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAged, 80 and overAsymptomatic InfectionsBlotting, SouthernChildCluster AnalysisColombiaDNA, HelminthDNA, KinetoplastFemaleGenetic VariationGenotypeHumansLeishmaniaLeishmaniasis, CutaneousMaleMiddle AgedMolecular Sequence DataPhylogenyPolymerase Chain ReactionRNA, Small CytoplasmicSequence Analysis, DNASignal Recognition ParticleYoung AdultConceptsCutaneous leishmaniasisSubclinical infectionParasitological confirmationAsymptomatic infectionEndemic areasHistory of CLTest-positive individualsImmunological evidenceViability of LeishmaniaMucosal swab samplesPersistent subclinical infectionMucosal tissue samplesReservoir of infectionActive diseaseLeishmania kDNALeishmania infectionPositive individualsPersistent infectionBlood monocytesParasite burdenInfectionParasite populationsSwab samplesTransmission of diseaseTissue samples
2010
Viability and Burden of Leishmania in Extralesional Sites during Human Dermal Leishmaniasis
Romero I, Téllez J, Suárez Y, Cardona M, Figueroa R, Zelazny A, Saravia N. Viability and Burden of Leishmania in Extralesional Sites during Human Dermal Leishmaniasis. PLOS Neglected Tropical Diseases 2010, 4: e819. PMID: 20856851, PMCID: PMC2939031, DOI: 10.1371/journal.pntd.0000819.Peer-Reviewed Original ResearchConceptsLeishmaniasis patientsExtralesional sitesNormal skinTonsil swabsViable parasitesDissemination of LeishmaniaMucocutaneous leishmaniasis patientsMonocyte samplesPositive samplesLuciferase activityParasite viabilitySkin aspiratesActive diseaseDermal leishmaniasisHuman leishmaniasisBlood monocytesEpidemiologic investigationsPatientsLeishmania DNAParasite burdenHuman infectionsIntracellular amastigotesLesionsSwabsEpidemiological significance
1998
Permissiveness of human monocytes and monocyte-derived macrophages to infection by promastigotes of Leishmania (Viannia) panamensis.
Bosque F, Milon G, Valderrama L, Saravia N. Permissiveness of human monocytes and monocyte-derived macrophages to infection by promastigotes of Leishmania (Viannia) panamensis. Journal Of Parasitology 1998, 84: 1250-6. PMID: 9920322, DOI: 10.2307/3284682.Peer-Reviewed Original ResearchConceptsPercentage of cellsBlood monocytesInduction of differentiationLeishmania panamensis infectionHuman peripheral blood monocytesHistiocytic cell line UDifferentiated macrophagesExpression of CD68Number of amastigotesPeripheral blood monocytesOutcome of infectionSite of infectionMonocyte-derived macrophagesL. panamensis promastigotesMononuclear phagocytic cellsPhorbol myristate acetatePanamensis infectionNumber of parasitesCell line UBone marrowFresh monocytesLeishmania panamensisMonocytesResident macrophagesStages of differentiation
1994
Human monocyte infection by Leishmania (Viannia) panamensis. Role of complement receptors and correlation of susceptibility in vitro with clinical phenotype.
Robledo S, Wozencraft A, Valencia A, Saravia N. Human monocyte infection by Leishmania (Viannia) panamensis. Role of complement receptors and correlation of susceptibility in vitro with clinical phenotype. The Journal Of Immunology 1994, 152: 1265-76. PMID: 8301130, DOI: 10.4049/jimmunol.152.3.1265.Peer-Reviewed Original ResearchConceptsPeripheral blood monocytesClinical phenotypeHuman monocytesDistinctive clinical outcomesDifferent clinical groupsComplement receptors CR1Human monocyte cell line UPercentage of cellsPatterns of susceptibilityMonocyte infectionPanamensis infectionClinical outcomesDisease 5Number of parasitesStationary phase promastigotesBlood monocytesComplement receptorsCell line UHealthy individualsReceptors CR1Leishmania panamensisExperimental infectionInfectionUnexposed controlsAntireceptor antibodies