2023
CLIP-Seq analysis enables the design of protective ribosomal RNA bait oligonucleotides against C9ORF72 ALS/FTD poly-GR pathophysiology
Ortega J, Sasselli I, Boccitto M, Fleming A, Fortuna T, Li Y, Sato K, Clemons T, Mckenna E, Nguyen T, Anderson E, Asin J, Ichida J, Pandey U, Wolin S, Stupp S, Kiskinis E. CLIP-Seq analysis enables the design of protective ribosomal RNA bait oligonucleotides against C9ORF72 ALS/FTD poly-GR pathophysiology. Science Advances 2023, 9: eadf7997. PMID: 37948524, PMCID: PMC10637751, DOI: 10.1126/sciadv.adf7997.Peer-Reviewed Original Research
2018
Bacterial Y RNAs: Gates, Tethers, and tRNA Mimics
Sim S, Wolin S. Bacterial Y RNAs: Gates, Tethers, and tRNA Mimics. 2018, 369-381. DOI: 10.1128/9781683670247.ch21.Peer-Reviewed Original ResearchY RNAsBacterial Y RNAsRing-shaped proteinSubset of bacteriaCharacterized organismsProtein partnersNoncoding RNAsTRNA mimicAnimal cellsHomology searchHuman RNAHuman cellsRNANucleotidesImportant targetBacteriaSystemic autoimmune rheumatic diseasesAutoimmune rheumatic diseasesSystemic lupus erythematosusCellsRNAsOrganismsSpeciesProteinLupus erythematosus084 The role of interferon and retroelements in lupus-prone Ro60 knockout mouse skin
Rokunohe D, Chiou E, Sun X, Tanaka L, Wolin S, Elkon K, Kawasumi M. 084 The role of interferon and retroelements in lupus-prone Ro60 knockout mouse skin. Journal Of Investigative Dermatology 2018, 138: s14. DOI: 10.1016/j.jid.2018.03.088.Peer-Reviewed Original Research
2017
068 Lupus Ro60 autoantigen cross-reactivity with commensal Ro60 orthologs
Greiling T, Dehner C, Chen X, Hughes K, Vieira S, Ruff W, Sim S, Wolin S, Kriegel M. 068 Lupus Ro60 autoantigen cross-reactivity with commensal Ro60 orthologs. Journal Of Investigative Dermatology 2017, 137: s12. DOI: 10.1016/j.jid.2017.02.081.Peer-Reviewed Original ResearchCommensal Ro60 Orthologs as Persistent Triggers of Human Lupus
Dehner C, Greiling T, Chen X, Renfroe S, Hughes K, Vieira S, Ruff W, Boccitto M, Sim S, Chen X, Kriegel C, Degnan P, Goodman A, Wolin S, Kriegel M. Commensal Ro60 Orthologs as Persistent Triggers of Human Lupus. The FASEB Journal 2017, 31 DOI: 10.1096/fasebj.31.1_supplement.55.3.Peer-Reviewed Original ResearchMesenteric lymph nodesSystemic lupus erythematosusT cell clonesLupus patientsSLE patientsTg miceAutoimmune diseasesMicrobial triggersCell clonesT cellsGerm-free (GF) C57BL/6 miceP. propionicumMemory CD4 T cellsHuman SLE seraPrototypical autoimmune diseaseCD4 T cellsHuman autoimmune diseasesGerm-free miceAnti-Ro60 antibodiesEarliest autoantibodiesRo60 antibodiesLupus modelsLupus erythematosusLymph nodesHuman lupus
2015
A retrovirus packages nascent host noncoding RNAs from a novel surveillance pathway
Eckwahl MJ, Sim S, Smith D, Telesnitsky A, Wolin SL. A retrovirus packages nascent host noncoding RNAs from a novel surveillance pathway. Genes & Development 2015, 29: 646-657. PMID: 25792599, PMCID: PMC4378196, DOI: 10.1101/gad.258731.115.Peer-Reviewed Original ResearchConceptsSmall nuclear RNAMoloney leukemia virusSurveillance pathwayU6 small nuclear RNASmall nucleolar RNAsHigh-throughput sequencingHost cell RNAExoribonuclease DIS3L2RNA exosomeLeukemia virusExportin-5Nucleolar RNAsNuclear RNAHost RNASpecific tRNAsCytoplasmic recruitmentMurine leukemia virusCell RNAEndogenous retrovirusesRNAModel retrovirusRNAsVirionsCytoplasmPathway
2014
Bacterial noncoding Y RNAs are widespread and mimic tRNAs
Chen X, Sim S, Wurtmann EJ, Feke A, Wolin SL. Bacterial noncoding Y RNAs are widespread and mimic tRNAs. RNA 2014, 20: 1715-1724. PMID: 25232022, PMCID: PMC4201824, DOI: 10.1261/rna.047241.114.Peer-Reviewed Original ResearchConceptsY RNAsStructured RNA degradationRing-shaped proteinNoncoding Y RNAsBacterial physiologyAnimal cellsNucleotide modificationsDeinococcus radioduransPhage speciesRNA degradationTRNARo60 autoantigenRNAOrthologsNcRNAsSpeciesBacteriaExoribonucleaseRNAsRadioduransProteinRo60EnzymePhysiologyPhosphorylase
2013
An RNA Degradation Machine Sculpted by Ro Autoantigen and Noncoding RNA
Chen X, Taylor DW, Fowler CC, Galan JE, Wang HW, Wolin SL. An RNA Degradation Machine Sculpted by Ro Autoantigen and Noncoding RNA. Cell 2013, 153: 166-177. PMID: 23540697, PMCID: PMC3646564, DOI: 10.1016/j.cell.2013.02.037.Peer-Reviewed Original ResearchConceptsRNA degradation machineDegradation machineY RNAsSingle-particle electron microscopyRing-shaped proteinRibosomal RNA maturationExoribonuclease polynucleotide phosphorylaseRo autoantigenNcRNAs actRRNA decayRNA maturationProtein cofactorsNoncoding RNAsSubstrate specificityDeinococcus radioduransStructured RNAsPolynucleotide phosphorylaseBiochemical assaysRNAOrthologsNcRNAPNPaseProteinSalmonella typhimuriumAtomic model
2012
An MBoC Favorite: The historic covers selected by Joseph Gall that graced MBoC from 1992 to 1996
Wolin S. An MBoC Favorite: The historic covers selected by Joseph Gall that graced MBoC from 1992 to 1996. Molecular Biology Of The Cell 2012, 23: 1797-1797. PMCID: PMC3350544, DOI: 10.1091/mbc.e12-02-0141.Peer-Reviewed Original ResearchNuclear noncoding RNA surveillance: is the end in sight?
Wolin SL, Sim S, Chen X. Nuclear noncoding RNA surveillance: is the end in sight? Trends In Genetics 2012, 28: 306-313. PMID: 22475369, PMCID: PMC3378728, DOI: 10.1016/j.tig.2012.03.005.Peer-Reviewed Original ResearchConceptsRNA surveillance pathwaySurveillance pathwayAberrant RNAsSteady-state transcriptomeYeast counterpartEukaryotic cellsProtein cofactorsMammalian cellsRNA determinantsHuman diseasesSubsequent degradationRNACofactorPathwayKey roleRecent studiesMetazoansExoribonucleasesAccessible endTranscriptomeNcRNAsCellsYeastNucleaseProtein
2011
The zipcode-binding protein ZBP1 influences the subcellular location of the Ro 60-kDa autoantigen and the noncoding Y3 RNA
Sim S, Yao J, Weinberg DE, Niessen S, Yates JR, Wolin SL. The zipcode-binding protein ZBP1 influences the subcellular location of the Ro 60-kDa autoantigen and the noncoding Y3 RNA. RNA 2011, 18: 100-110. PMID: 22114317, PMCID: PMC3261732, DOI: 10.1261/rna.029207.111.Peer-Reviewed Original ResearchConceptsY3 RNASubcellular locationCRM1 inhibitor leptomycin B.RNA-binding proteinExport signalVertebrate nucleiVertebrate cellsY RNAsRNA bindingLeptomycin B.Nuclear exportSubcellular localizationNoncoding RNAsRNA complexZBP1Ro proteinCellular componentsRNACytoplasmProteinNucleusCRM1Complex increasesExportUV irradiationAn intrinsically disordered C terminus allows the La protein to assist the biogenesis of diverse noncoding RNA precursors
Kucera NJ, Hodsdon ME, Wolin SL. An intrinsically disordered C terminus allows the La protein to assist the biogenesis of diverse noncoding RNA precursors. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 1308-1313. PMID: 21212361, PMCID: PMC3029687, DOI: 10.1073/pnas.1017085108.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnticodonChymotrypsinElectrophoretic Mobility Shift AssayImmunoblottingModels, MolecularMolecular Sequence DataMutationNucleic Acid ConformationProtein BindingProtein Structure, TertiaryRNA PrecursorsRNA, FungalRNA, RibosomalRNA, Small NuclearRNA, TransferRNA, UntranslatedRNA-Binding ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidTrypsin
2008
Running Rings Around RNA: The role of Ro RNPs in RNA maturation and decay
Wolin S. Running Rings Around RNA: The role of Ro RNPs in RNA maturation and decay. The FASEB Journal 2008, 22: 527.3-527.3. DOI: 10.1096/fasebj.22.1_supplement.527.3.Peer-Reviewed Original ResearchY RNAsRNA bindsAnimal cellsRNA binding proteinRNA quality controlY-RNAsRo RNPsRNA maturationVertebrate cellsSmall RNAsCertain prokaryotesMammalian cellsD. radioduransLikely functionsStructured RNAsRunning ringsHelical portionBinding proteinRNABiochemical studiesEfficient maturationCentral cavityBacteriaProteinBinds
2006
Molecular Chaperones and Quality Control in Noncoding RNA Biogenesis
WOLIN S, WURTMANN E. Molecular Chaperones and Quality Control in Noncoding RNA Biogenesis. Cold Spring Harbor Symposia On Quantitative Biology 2006, 71: 505-511. PMID: 17381333, DOI: 10.1101/sqb.2006.71.051.Peer-Reviewed Original ResearchConceptsRNA quality controlNoncoding RNAsMolecular chaperonesSm-like protein HfqQuality control pathwaysRNA biogenesisProtein HfqCorrect foldingEfficient foldingAnimal cellsExonucleolytic degradationLikely functionsLa proteinControl pathwaysQuality control systemRo proteinRNACertain bacteriaProteinChaperonesCritical roleFoldingFunctional structureBacteriaQuality control
2001
Phosphorylation of the Saccharomyces cerevisiae La protein does not appear to be required for its functions in tRNA maturation and nascent RNA stabilization.
Long K, Cedervall T, Walch-Solimena C, Noe D, Huddleston M, Annan R, Wolin S. Phosphorylation of the Saccharomyces cerevisiae La protein does not appear to be required for its functions in tRNA maturation and nascent RNA stabilization. RNA 2001, 7: 1589-602. PMID: 11720288, PMCID: PMC1370201.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAutoantigensBinding SitesCell NucleolusCell NucleusFungal ProteinsMolecular Sequence DataPeptide MappingPhosphorylationProtein IsoformsRibonucleoproteinsRibonucleoproteins, Small NuclearRNARNA StabilityRNA, FungalRNA, TransferRNA-Binding ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsConceptsLa proteinAbundant nuclear phosphoproteinRNA polymerase III transcriptsS. cerevisiae proteinTwo-dimensional gel electrophoresisRole of phosphorylationPolymerase III transcriptsCerevisiae proteinsNascent RNANascent transcriptsS. pombeSchizosaccharomyces pombeLhp1pPhosphorylation sitesYeast SaccharomycesProtein functionMutant versionSubcellular locationFirst proteinHuman proteinsNuclear phosphoproteinExonucleolytic degradationSerine phosphorylationPhosphorylation statusRNA stabilizationMultiple Functional Interactions Between Components of the Lsm2-Lsm8 Complex, U6 snRNA, and the Yeast La Protein
Pannone B, Do Kim S, Noe D, Wolin S. Multiple Functional Interactions Between Components of the Lsm2-Lsm8 Complex, U6 snRNA, and the Yeast La Protein. Genetics 2001, 158: 187-196. PMID: 11333229, PMCID: PMC1461625, DOI: 10.1093/genetics/158.1.187.Peer-Reviewed Original ResearchConceptsU6 snRNALa proteinLow copy suppressorU6 small nuclear ribonucleoproteinYeast La proteinSm-like proteinsFunctional interactionOnly essential functionU6 snRNA genesSmall nuclear ribonucleoproteinMultiple functional interactionsLsm geneRNA biogenesisLsm proteinsSnRNA genesEukaryotic spliceosomeSmall RNAsSm motifsAbundant phosphoproteinLsm8pU6 snRNPsFirst proteinNuclear ribonucleoproteinLhp1pSnRNA
2000
RNA degradation: Sm-like proteins wRING the neck of mRNA
Pannone B, Wolin S. RNA degradation: Sm-like proteins wRING the neck of mRNA. Current Biology 2000, 10: r478-r481. PMID: 10898971, DOI: 10.1016/s0960-9822(00)00552-2.Peer-Reviewed Original ResearchU snRNP assembly in yeast involves the La protein
Xue D, Rubinson D, Pannone B, Yoo C, Wolin S. U snRNP assembly in yeast involves the La protein. The EMBO Journal 2000, 19: 2763-2763. DOI: 10.1093/emboj/19.11.2763.Peer-Reviewed Original ResearchU snRNP assembly in yeast involves the La protein
Xue D, Rubinson D, Pannone B, Yoo C, Wolin S. U snRNP assembly in yeast involves the La protein. The EMBO Journal 2000, 19: 1650-1660. PMID: 10747032, PMCID: PMC310233, DOI: 10.1093/emboj/19.7.1650.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceDNA PrimersFungal ProteinsGenes, FungalMutationProtein BindingRibonucleoprotein, U1 Small NuclearRibonucleoprotein, U4-U6 Small NuclearRibonucleoproteins, Small NuclearRNA PrecursorsRNA StabilityRNA, FungalRNA-Binding ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSpliceosomesConceptsU4 RNALa proteinU4/U6 snRNPLa autoantigen bindsU snRNP assemblyRNA polymerase III transcriptsU4/U6 snRNPsRNA polymerase IIPolymerase III transcriptsEukaryotic nucleusSnRNP assemblySpliceosomal snRNPsSm proteinsU5 snRNPsMature RNAPolymerase IIU5 RNALhp1pU6 snRNPMutant cellsU6 snRNPsPermissive temperatureYeast cellsSnRNPsRNARo ribonucleoproteins contribute to the resistance of Deinococcus radiodurans to ultraviolet irradiation
Chen X, Quinn A, Wolin S. Ro ribonucleoproteins contribute to the resistance of Deinococcus radiodurans to ultraviolet irradiation. Genes & Development 2000, 14: 777-782. PMID: 10766734, PMCID: PMC316496, DOI: 10.1101/gad.14.7.777.Peer-Reviewed Original Research