2012
Nuclear noncoding RNA surveillance: is the end in sight?
Wolin SL, Sim S, Chen X. Nuclear noncoding RNA surveillance: is the end in sight? Trends In Genetics 2012, 28: 306-313. PMID: 22475369, PMCID: PMC3378728, DOI: 10.1016/j.tig.2012.03.005.Peer-Reviewed Original ResearchConceptsRNA surveillance pathwaySurveillance pathwayAberrant RNAsSteady-state transcriptomeYeast counterpartEukaryotic cellsProtein cofactorsMammalian cellsRNA determinantsHuman diseasesSubsequent degradationRNACofactorPathwayKey roleRecent studiesMetazoansExoribonucleasesAccessible endTranscriptomeNcRNAsCellsYeastNucleaseProtein
2011
The zipcode-binding protein ZBP1 influences the subcellular location of the Ro 60-kDa autoantigen and the noncoding Y3 RNA
Sim S, Yao J, Weinberg DE, Niessen S, Yates JR, Wolin SL. The zipcode-binding protein ZBP1 influences the subcellular location of the Ro 60-kDa autoantigen and the noncoding Y3 RNA. RNA 2011, 18: 100-110. PMID: 22114317, PMCID: PMC3261732, DOI: 10.1261/rna.029207.111.Peer-Reviewed Original ResearchConceptsY3 RNASubcellular locationCRM1 inhibitor leptomycin B.RNA-binding proteinExport signalVertebrate nucleiVertebrate cellsY RNAsRNA bindingLeptomycin B.Nuclear exportSubcellular localizationNoncoding RNAsRNA complexZBP1Ro proteinCellular componentsRNACytoplasmProteinNucleusCRM1Complex increasesExportUV irradiation
2001
Phosphorylation of the Saccharomyces cerevisiae La protein does not appear to be required for its functions in tRNA maturation and nascent RNA stabilization.
Long K, Cedervall T, Walch-Solimena C, Noe D, Huddleston M, Annan R, Wolin S. Phosphorylation of the Saccharomyces cerevisiae La protein does not appear to be required for its functions in tRNA maturation and nascent RNA stabilization. RNA 2001, 7: 1589-602. PMID: 11720288, PMCID: PMC1370201.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAutoantigensBinding SitesCell NucleolusCell NucleusFungal ProteinsMolecular Sequence DataPeptide MappingPhosphorylationProtein IsoformsRibonucleoproteinsRibonucleoproteins, Small NuclearRNARNA StabilityRNA, FungalRNA, TransferRNA-Binding ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsConceptsLa proteinAbundant nuclear phosphoproteinRNA polymerase III transcriptsS. cerevisiae proteinTwo-dimensional gel electrophoresisRole of phosphorylationPolymerase III transcriptsCerevisiae proteinsNascent RNANascent transcriptsS. pombeSchizosaccharomyces pombeLhp1pPhosphorylation sitesYeast SaccharomycesProtein functionMutant versionSubcellular locationFirst proteinHuman proteinsNuclear phosphoproteinExonucleolytic degradationSerine phosphorylationPhosphorylation statusRNA stabilization
2000
Import of proteins into the trypanosome nucleus and their distribution at karyokinesis
Marchetti M, Tschudi C, Kwon H, Wolin S, Ullu E. Import of proteins into the trypanosome nucleus and their distribution at karyokinesis. Journal Of Cell Science 2000, 113: 899-906. PMID: 10671379, DOI: 10.1242/jcs.113.5.899.Peer-Reviewed Original ResearchConceptsNuclear localization sequenceMonopartite nuclear localization sequenceBipartite nuclear localization sequenceFusion proteinSpecific nuclear localization sequencesImport of proteinsEarly divergent eukaryoteGreen fluorescent proteinDivergent eukaryotesOrganism's proteinsChromosome segregationBeta-galactosidase reporterTrypanosomatid protozoaProtein homologueTrypanosomal proteinsHistone H2BHistone H2B.Localization sequenceNuclear divisionLa proteinC-terminusTrypanosome nucleusMitotic cellsFluorescent proteinATP synthesis
1999
The trials and travels of tRNA
Wolin S, Matera A. The trials and travels of tRNA. Genes & Development 1999, 13: 1-10. PMID: 9887094, DOI: 10.1101/gad.13.1.1.Peer-Reviewed Original Research