1993
Discrete nascent chain lengths are required for the insertion of presecretory proteins into microsomal membranes.
Wolin S, Walter P. Discrete nascent chain lengths are required for the insertion of presecretory proteins into microsomal membranes. Journal Of Cell Biology 1993, 121: 1211-1219. PMID: 8389768, PMCID: PMC2119713, DOI: 10.1083/jcb.121.6.1211.Peer-Reviewed Original ResearchConceptsSignal recognition particlePresecretory proteinsER membraneInteraction of SRPSecretory proteinsNascent chain lengthNascent secretory proteinsMembrane-associated ribosomesTranslation of mRNAsMicrosomal membranesMembrane-bound ribosomesRecognition particleSmall ribonucleoproteinTranslation arrestSignal peptideRibosomesBovine preprolactinProteinHigh saltMembraneFurther elongationChain insertionPreprolactinRibonucleoproteinTranslation
1989
Signal recognition particle mediates a transient elongation arrest of preprolactin in reticulocyte lysate.
Wolin S, Walter P. Signal recognition particle mediates a transient elongation arrest of preprolactin in reticulocyte lysate. Journal Of Cell Biology 1989, 109: 2617-2622. PMID: 2556403, PMCID: PMC2115964, DOI: 10.1083/jcb.109.6.2617.Peer-Reviewed Original ResearchConceptsSignal recognition particleReticulocyte lysateRecognition particleMammalian signal recognition particleWheat germ translation extractsCanine pancreatic microsomal membranesTargeting of ribosomesPancreatic microsomal membranesWheat germ extractSRP bindsPresecretory proteinsElongation arrestER membraneTranslation arrestSignal sequenceTranslation extractsSecretory proteinsGerm extractRibosomesPreprolactin mRNAMicrosomal membranesLysatesSpecific sitesProteinFurther elongation