1998
Analyses of Ribosome Distribution During In Vitro Translation
Wolin S. Analyses of Ribosome Distribution During In Vitro Translation. Methods In Molecular Biology 1998, 77: 1-9. PMID: 9770657, DOI: 10.1385/0-89603-397-x:1.Peer-Reviewed Original ResearchConceptsRibosome distributionFull-length cDNA cloneSignal recognition particleArrest of translationSingle-nucleotide precisionPosition of ribosomesDistribution of ribosomesRibosome pausesRecognition particleRibosome stallingParticular mRNA speciesTranslational regulationNucleotide precisionVitro translationCDNA clonesAbundant mRNAsMRNA speciesTermination codonRibosomesMRNA sequencesMRNAAssaysTranslationCodonSpecies
1993
Discrete nascent chain lengths are required for the insertion of presecretory proteins into microsomal membranes.
Wolin S, Walter P. Discrete nascent chain lengths are required for the insertion of presecretory proteins into microsomal membranes. Journal Of Cell Biology 1993, 121: 1211-1219. PMID: 8389768, PMCID: PMC2119713, DOI: 10.1083/jcb.121.6.1211.Peer-Reviewed Original ResearchConceptsSignal recognition particlePresecretory proteinsER membraneInteraction of SRPSecretory proteinsNascent chain lengthNascent secretory proteinsMembrane-associated ribosomesTranslation of mRNAsMicrosomal membranesMembrane-bound ribosomesRecognition particleSmall ribonucleoproteinTranslation arrestSignal peptideRibosomesBovine preprolactinProteinHigh saltMembraneFurther elongationChain insertionPreprolactinRibonucleoproteinTranslation
1989
Signal recognition particle mediates a transient elongation arrest of preprolactin in reticulocyte lysate.
Wolin S, Walter P. Signal recognition particle mediates a transient elongation arrest of preprolactin in reticulocyte lysate. Journal Of Cell Biology 1989, 109: 2617-2622. PMID: 2556403, PMCID: PMC2115964, DOI: 10.1083/jcb.109.6.2617.Peer-Reviewed Original ResearchConceptsSignal recognition particleReticulocyte lysateRecognition particleMammalian signal recognition particleWheat germ translation extractsCanine pancreatic microsomal membranesTargeting of ribosomesPancreatic microsomal membranesWheat germ extractSRP bindsPresecretory proteinsElongation arrestER membraneTranslation arrestSignal sequenceTranslation extractsSecretory proteinsGerm extractRibosomesPreprolactin mRNAMicrosomal membranesLysatesSpecific sitesProteinFurther elongation