2021
Reprogramming of bivalent chromatin states in NRAS mutant melanoma suggests PRC2 inhibition as a therapeutic strategy
Terranova C, Tang M, Maitituoheti M, Raman A, Ghosh A, Schulz J, Amin S, Orouji E, Tomczak K, Sarkar S, Oba J, Creasy C, Wu C, Khan S, Lazcano R, Wani K, Singh A, Barrodia P, Zhao D, Chen K, Haydu L, Wang W, Lazar A, Woodman S, Bernatchez C, Rai K. Reprogramming of bivalent chromatin states in NRAS mutant melanoma suggests PRC2 inhibition as a therapeutic strategy. Cell Reports 2021, 36: 109410. PMID: 34289358, PMCID: PMC8369408, DOI: 10.1016/j.celrep.2021.109410.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Line, TumorCell ProliferationChromatinEnhancer of Zeste Homolog 2 ProteinFemaleGTP PhosphohydrolasesHistonesHumansMelanocytesMelanomaMembrane ProteinsMesodermMice, NudeMitogen-Activated Protein Kinase KinasesMutationNeoplasm MetastasisPolycomb Repressive Complex 2Transcription, GeneticTumor BurdenConceptsHistone H3 lysine 27 trimethylationH3 lysine 27 trimethylationBivalent chromatin stateCell identity genesLysine 27 trimethylationKey epigenetic alterationsNRAS mutantsMaster transcription factorBivalent domainsChromatin statePRC2 inhibitionEpigenetic elementsTranscription factorsEpigenetic alterationsGenetic driversMesenchymal phenotypeNRAS-mutant melanomaState profilingTherapeutic vulnerabilitiesInvasive capacityPharmacological inhibitionMutantsTherapeutic strategiesMelanoma samplesMutant melanoma patients
2013
Transcription factor-pathway coexpression analysis reveals cooperation between SP1 and ESR1 on dysregulating cell cycle arrest in non-hyperdiploid multiple myeloma
Wang X, Yan Z, Fulciniti M, Li Y, Gkotzamanidou M, Amin S, Shah P, Zhang Y, Munshi N, Li C. Transcription factor-pathway coexpression analysis reveals cooperation between SP1 and ESR1 on dysregulating cell cycle arrest in non-hyperdiploid multiple myeloma. Leukemia 2013, 28: 894-903. PMID: 23925045, PMCID: PMC4155324, DOI: 10.1038/leu.2013.233.Peer-Reviewed Original ResearchConceptsCell cycle arrestCycle arrestCoexpression analysisCell cycle arrest genesHyperdiploid MMCell cycle arrest pathwaysNon-hyperdiploid multiple myelomaDistinct chromosomal alterationsMyeloma subtypeMultiple myelomaTranscription factorsArrest pathwaysSp1Low coexpressionProper regulationHuman cancersDifferent survival outcomesChromosomal alterationsPlasma B cellsCoexpressionCell linesNovel hypothesisSurvival outcomesMyeloma proliferationClinical utility
2012
Integrative analysis of gene and miRNA expression profiles with transcription factor–miRNA feed-forward loops identifies regulators in human cancers
Yan Z, Shah P, Amin S, Samur M, Huang N, Wang X, Misra V, Ji H, Gabuzda D, Li C. Integrative analysis of gene and miRNA expression profiles with transcription factor–miRNA feed-forward loops identifies regulators in human cancers. Nucleic Acids Research 2012, 40: e135-e135. PMID: 22645320, PMCID: PMC3458521, DOI: 10.1093/nar/gks395.Peer-Reviewed Original ResearchConceptsFeed-forward loopTranscription factorsMiRNA expression profilesExpression profilesNovel feed-forward loopCancer-related transcription factorsExpression dataTF target genesMiRNA-mRNA interactionsCommon target genesMiR-15/miRMiRNA expression dataMiRNA partnersTranscriptome changesTarget genesDifferential genesIntegrative analysisMultiple cancer typesGenesMiRNA expressionHuman cancersLiterature validationBiological conditionsMiRNAsRegulator
2011
Significant Biological Role of Sp1 Transactivation in Multiple Myeloma
Fulciniti M, Amin S, Nanjappa P, Rodig S, Prabhala R, Li C, Minvielle S, Tai Y, Tassone P, Avet-Loiseau H, Hideshima T, Anderson K, Munshi N. Significant Biological Role of Sp1 Transactivation in Multiple Myeloma. Clinical Cancer Research 2011, 17: 6500-6509. PMID: 21856768, PMCID: PMC4318245, DOI: 10.1158/1078-0432.ccr-11-1036.Peer-Reviewed Original ResearchConceptsBone marrow stromal cellsSp1 activitySp1 knockdownTranscription factor specificity protein 1Caspase-9-dependent apoptosisCritical cell cycleSp1 DNA bindingSp1-responsive promotersSpecificity protein 1Cell growthFirefly luciferase reporter geneImportant transcription factorImportant regulatory roleLuciferase reporter geneSignificant biological roleApoptosis-related genesSp1 transactivationShort hairpin RNASp1 DNACellular processesTranscription factorsPromoter elementsMarrow stromal cellsReporter geneMM cells
2010
Targeting Sp1 Transactivation In Waldenstrom's Macroglobulinemia: a Novel Therapeutic Option
Fulciniti M, Amin S, Mohan V, Yang G, Nanjappa P, Tassone P, Prabhala R, Cheng L, Anderson K, Treon S, Munshi N. Targeting Sp1 Transactivation In Waldenstrom's Macroglobulinemia: a Novel Therapeutic Option. Blood 2010, 116: 120. DOI: 10.1182/blood.v116.21.120.120.Peer-Reviewed Original ResearchBone marrow stromal cellsSp1 activityWM cellsCell growthSp1 protein expressionSp1 protein levelsTranscription factor Sp1Lentiviral shRNA constructsGC-rich motifsCaspase-8 activationImportant transcription factorMitochondrial apoptotic pathwayExpression of genesAnti-apoptotic genesGene promoter regionHigh Sp1 expressionSp1 transactivationLuciferase reporter plasmidFactor Sp1Sp proteinsSp1 inhibitorDownregulation of expressionSp1 expressionTranscription factorsPromoter elementsBiology and Therapeutic Targeting of Sp1 Transactivation In Myeloma
Fulciniti M, Amin S, Nanjappa P, Rodig S, Hideshima T, Pal J, Mohan V, Lee K, Shammas M, Minvielle S, Prabhala R, Avet-Loiseau H, Cheng L, Anderson K, Munshi N. Biology and Therapeutic Targeting of Sp1 Transactivation In Myeloma. Blood 2010, 116: 134. DOI: 10.1182/blood.v116.21.134.134.Peer-Reviewed Original ResearchSp1 activityTranscription factorsCell growthApoptotic pathwayOverexpression of Sp1Role of Sp1GC-rich motifsGene expression profilesMitochondrial apoptotic pathwayPathway inhibitor U0126ERK pathway inhibitor U0126Important regulatory roleSpecific inhibitionSp1 transactivationMAPK genesSp1 siRNAMM cell growthSp1 activationMM cellsCellular processesNuclear localizationDNA bindingSp1Transcriptional activityPromoter activity