2011
Significant Biological Role of Sp1 Transactivation in Multiple Myeloma
Fulciniti M, Amin S, Nanjappa P, Rodig S, Prabhala R, Li C, Minvielle S, Tai Y, Tassone P, Avet-Loiseau H, Hideshima T, Anderson K, Munshi N. Significant Biological Role of Sp1 Transactivation in Multiple Myeloma. Clinical Cancer Research 2011, 17: 6500-6509. PMID: 21856768, PMCID: PMC4318245, DOI: 10.1158/1078-0432.ccr-11-1036.Peer-Reviewed Original ResearchConceptsBone marrow stromal cellsSp1 activitySp1 knockdownTranscription factor specificity protein 1Caspase-9-dependent apoptosisCritical cell cycleSp1 DNA bindingSp1-responsive promotersSpecificity protein 1Cell growthFirefly luciferase reporter geneImportant transcription factorImportant regulatory roleLuciferase reporter geneSignificant biological roleApoptosis-related genesSp1 transactivationShort hairpin RNASp1 DNACellular processesTranscription factorsPromoter elementsMarrow stromal cellsReporter geneMM cells
2010
Targeting Sp1 Transactivation In Waldenstrom's Macroglobulinemia: a Novel Therapeutic Option
Fulciniti M, Amin S, Mohan V, Yang G, Nanjappa P, Tassone P, Prabhala R, Cheng L, Anderson K, Treon S, Munshi N. Targeting Sp1 Transactivation In Waldenstrom's Macroglobulinemia: a Novel Therapeutic Option. Blood 2010, 116: 120. DOI: 10.1182/blood.v116.21.120.120.Peer-Reviewed Original ResearchBone marrow stromal cellsSp1 activityWM cellsCell growthSp1 protein expressionSp1 protein levelsTranscription factor Sp1Lentiviral shRNA constructsGC-rich motifsCaspase-8 activationImportant transcription factorMitochondrial apoptotic pathwayExpression of genesAnti-apoptotic genesGene promoter regionHigh Sp1 expressionSp1 transactivationLuciferase reporter plasmidFactor Sp1Sp proteinsSp1 inhibitorDownregulation of expressionSp1 expressionTranscription factorsPromoter elements