2011
Significant Biological Role of Sp1 Transactivation in Multiple Myeloma
Fulciniti M, Amin S, Nanjappa P, Rodig S, Prabhala R, Li C, Minvielle S, Tai Y, Tassone P, Avet-Loiseau H, Hideshima T, Anderson K, Munshi N. Significant Biological Role of Sp1 Transactivation in Multiple Myeloma. Clinical Cancer Research 2011, 17: 6500-6509. PMID: 21856768, PMCID: PMC4318245, DOI: 10.1158/1078-0432.ccr-11-1036.Peer-Reviewed Original ResearchConceptsBone marrow stromal cellsSp1 activitySp1 knockdownTranscription factor specificity protein 1Caspase-9-dependent apoptosisCritical cell cycleSp1 DNA bindingSp1-responsive promotersSpecificity protein 1Cell growthFirefly luciferase reporter geneImportant transcription factorImportant regulatory roleLuciferase reporter geneSignificant biological roleApoptosis-related genesSp1 transactivationShort hairpin RNASp1 DNACellular processesTranscription factorsPromoter elementsMarrow stromal cellsReporter geneMM cells
2010
Biology and Therapeutic Targeting of Sp1 Transactivation In Myeloma
Fulciniti M, Amin S, Nanjappa P, Rodig S, Hideshima T, Pal J, Mohan V, Lee K, Shammas M, Minvielle S, Prabhala R, Avet-Loiseau H, Cheng L, Anderson K, Munshi N. Biology and Therapeutic Targeting of Sp1 Transactivation In Myeloma. Blood 2010, 116: 134. DOI: 10.1182/blood.v116.21.134.134.Peer-Reviewed Original ResearchSp1 activityTranscription factorsCell growthApoptotic pathwayOverexpression of Sp1Role of Sp1GC-rich motifsGene expression profilesMitochondrial apoptotic pathwayPathway inhibitor U0126ERK pathway inhibitor U0126Important regulatory roleSpecific inhibitionSp1 transactivationMAPK genesSp1 siRNAMM cell growthSp1 activationMM cellsCellular processesNuclear localizationDNA bindingSp1Transcriptional activityPromoter activity