2008
Vaccine Protection by Live, Attenuated Simian Immunodeficiency Virus in the Absence of High-Titer Antibody Responses and High-Frequency Cellular Immune Responses Measurable in the Periphery
Mansfield K, Lang S, Gauduin M, Sanford H, Lifson J, Johnson R, Desrosiers R. Vaccine Protection by Live, Attenuated Simian Immunodeficiency Virus in the Absence of High-Titer Antibody Responses and High-Frequency Cellular Immune Responses Measurable in the Periphery. Journal Of Virology 2008, 82: 4135-4148. PMID: 18272584, PMCID: PMC2292994, DOI: 10.1128/jvi.00015-08.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, ViralInterferon-gammaIntestinal MucosaLymphoid TissueMacaca mulattaNeutralization TestsRetroviridae ProteinsRNA, ViralSAIDS VaccinesSequence DeletionSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusT-LymphocytesVaccines, AttenuatedViral Envelope ProteinsViral LoadViremiaConceptsAnti-SIV antibody responsesSimian immunodeficiency virusAntibody responseImmune responseVaccine protectionImmunodeficiency virusCopies/Gamma interferon enzyme-linked immunospotVaccine/challenge experimentsAttenuated simian immunodeficiency virusGut-associated lymphoid tissueHigh-titer antibody responsesAnti-SIV immune responsesEnzyme-linked immunospotMacrophage marker CD68Cellular immune responsesHumoral immune responseViral load measurementsWeeks of infectionNeutralization-resistant strainDouble-labeling experimentsVaccinated monkeysViral loadPeripheral bloodLymphoid tissue
2006
Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus
Kaur A, Sanford H, Garry D, Lang S, Klumpp S, Watanabe D, Bronson R, Lifson J, Rosati M, Pavlakis G, Felber B, Knipe D, Desrosiers R. Ability of herpes simplex virus vectors to boost immune responses to DNA vectors and to protect against challenge by simian immunodeficiency virus. Virology 2006, 357: 199-214. PMID: 16962628, PMCID: PMC1819472, DOI: 10.1016/j.virol.2006.08.007.Peer-Reviewed Original ResearchConceptsSimian immunodeficiency virusELISPOT responsesImmunodeficiency virusHSV vectorsHerpes simplex virus vectorsPlasma SIV RNAPlasma viremia levelsVector-based vaccinesDay of challengeSimplex virus vectorsNef fusion proteinRecombinant HSV vectorsSIV RNAIntravenous challengeViremia levelsAntibody titersVaccine approachesImmune responseRhesus macaquesProtective capacityMacaquesRecombinant herpesvirusDNA vectorsFusion proteinEnvA Genetic System for Rhesus Monkey Rhadinovirus: Use of Recombinant Virus To Quantitate Antibody-Mediated Neutralization
Bilello J, Morgan J, Damania B, Lang S, Desrosiers R. A Genetic System for Rhesus Monkey Rhadinovirus: Use of Recombinant Virus To Quantitate Antibody-Mediated Neutralization. Journal Of Virology 2006, 80: 1549-1562. PMID: 16415030, PMCID: PMC1346924, DOI: 10.1128/jvi.80.3.1549-1562.2006.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAnimalsAntibodies, ViralBase SequenceCell LineCloning, MolecularCosmidsDNA, ViralGene LibraryGenes, ReporterGenome, ViralGreen Fluorescent ProteinsHerpesviridae InfectionsHumansMacaca mulattaNeutralization TestsRecombination, GeneticRhadinovirusTumor Virus InfectionsConceptsRhesus monkey rhadinovirusGreen fluorescent proteinGenetic systemRhesus monkey fibroblastsGFP reporter geneMonkey fibroblastsGamma-2 herpesvirusKaposi's sarcoma-associated herpesvirusDifferent cell typesGenome reconstitutionSarcoma-associated herpesvirusCosmid clonesTerminal repeat regionR1 geneReporter geneRepeat regionFluorescent proteinExpression cassetteCell typesSEAP activityRRV infectivityRecombinant virusesGenomeB cellsGenes
2005
Immunization of Macaques with Single-Cycle Simian Immunodeficiency Virus (SIV) Stimulates Diverse Virus-Specific Immune Responses and Reduces Viral Loads after Challenge with SIVmac239
Evans D, Bricker J, Sanford H, Lang S, Carville A, Richardson B, Piatak M, Lifson J, Mansfield K, Desrosiers R. Immunization of Macaques with Single-Cycle Simian Immunodeficiency Virus (SIV) Stimulates Diverse Virus-Specific Immune Responses and Reduces Viral Loads after Challenge with SIVmac239. Journal Of Virology 2005, 79: 7707-7720. PMID: 15919923, PMCID: PMC1143664, DOI: 10.1128/jvi.79.12.7707-7720.2005.Peer-Reviewed Original ResearchConceptsSingle-Cycle Simian Immunodeficiency VirusesRNA copies/AIDS vaccine approachSingle-cycle SIVEnzyme-linked immunospotViral loadSimian immunodeficiency virusCopies/Immunodeficiency virusVaccine approachesGamma interferon enzyme-linked immunospotGag-specific T-cell responsesVirus-specific immune responsesMajor Histocompatibility Complex Class I DownregulationT cell proliferative responsesImmunization of macaquesPlasma viral loadT cell responsesMHC class IThird doseChronic phasePeripheral bloodWeek 8Immune responseControl animals
1995
Progression to AIDS in the absence of a gene for vpr or vpx
Gibbs J, Lackner A, Lang S, Simon M, Sehgal P, Daniel M, Desrosiers R. Progression to AIDS in the absence of a gene for vpr or vpx. Journal Of Virology 1995, 69: 2378-2383. PMID: 7884883, PMCID: PMC188910, DOI: 10.1128/jvi.69.4.2378-2383.1995.Peer-Reviewed Original ResearchConceptsLow virus burdenSimian immunodeficiency virusVirus burdenRhesus monkeysLymphocyte concentrationsPlasma antigenemiaHigh virus burdenLymph node morphologyImmunodeficiency virusDisease progressionSIVmac replicationPrimate lentivirusesVirus replicationWild-type virusNode morphologyAIDSVpx geneTerminal stageProgressionAntigenemiaCD4VpxMonkeysEarly spikeBurden
1993
Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus
Lang S, Weeger M, Stahl-Hennig C, Coulibaly C, Hunsmann G, Müller J, Müller-Hermelink H, Fuchs D, Wachter H, Daniel M. Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus. Journal Of Virology 1993, 67: 902-912. PMID: 8380472, PMCID: PMC237444, DOI: 10.1128/jvi.67.2.902-912.1993.Peer-Reviewed Original ResearchConceptsVpr variantsTAA stop codonVpr geneATG start codonSite-specific mutagenesisVpr mutant virusSignificant selective pressureImportance of VprStart codonSelective pressureVpr mutantsStop codonGenesPathogenic molecular cloneMolecular cloneCell linesVprTest animalsCEMx174 cell lineCodonNef geneSimilar kineticsVirus replicationReversionStop signal