2006
Infection and Persistence of Rhesus Monkey Rhadinovirus in Immortalized B-Cell Lines
Bilello J, Lang S, Wang F, Aster J, Desrosiers R. Infection and Persistence of Rhesus Monkey Rhadinovirus in Immortalized B-Cell Lines. Journal Of Virology 2006, 80: 3644-3649. PMID: 16537632, PMCID: PMC1440374, DOI: 10.1128/jvi.80.7.3644-3649.2006.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternB-LymphocytesCell Culture TechniquesCell Line, TransformedCell NucleusCell Transformation, ViralFibroblastsGenes, ReporterGenome, ViralGreen Fluorescent ProteinsHerpesviridae InfectionsHerpesvirus 4, HumanIn Situ Hybridization, FluorescenceMacaca mulattaRecombinant ProteinsRhadinovirusTime FactorsTumor Virus InfectionsVirus Replication
2000
Simian Immunodeficiency Virus Containing Mutations in N-Terminal Tyrosine Residues and in the PxxP Motif in Nef Replicates Efficiently in Rhesus Macaques
Carl S, Iafrate A, Lang S, Stolte N, Stahl-Hennig C, Mätz-Rensing K, Fuchs D, Skowronski J, Kirchhoff F. Simian Immunodeficiency Virus Containing Mutations in N-Terminal Tyrosine Residues and in the PxxP Motif in Nef Replicates Efficiently in Rhesus Macaques. Journal Of Virology 2000, 74: 4155-4164. PMID: 10756028, PMCID: PMC111930, DOI: 10.1128/jvi.74.9.4155-4164.2000.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesCD4 AntigensCell Line, TransformedCOS CellsDown-RegulationGene Products, nefHistocompatibility Antigens Class IHumansMacaca mulattaMutagenesisP21-Activated KinasesPhosphorylationProtein Serine-Threonine KinasesSimian immunodeficiency virusSrc Homology DomainsTyrosineViral LoadVirus ReplicationConceptsAbility of NefTyrosine-based endocytosis signalTyrosine residuesHigh viral loadCellular signal transductionTyrosine kinase SrcNef functionViral loadEndocytosis signalEndocytic machineryPXXP motifN-terminal tyrosine residueCell surface expressionKinase SrcKinase assaysSignal transductionProline residuesClass I major histocompatibility complexN-terminal tyrosineI major histocompatibility complexLigand domainCombined mutationsNef interactionsMajor histocompatibility complexMutations
1999
The Acidic Region and Conserved Putative Protein Kinase C Phosphorylation Site in Nef Are Important for SIV Replication in Rhesus Macaques
Carl S, Iafrate A, Lang S, Stahl-Hennig C, Kuhn E, Fuchs D, Mätz-Rensing K, Haaft P, Heeney J, Skowronski J, Kirchhoff F. The Acidic Region and Conserved Putative Protein Kinase C Phosphorylation Site in Nef Are Important for SIV Replication in Rhesus Macaques. Virology 1999, 257: 138-155. PMID: 10208928, DOI: 10.1006/viro.1999.9645.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCD4 AntigensCOS CellsGene Products, nefHistocompatibility Antigens Class IHIV-1Hydrogen-Ion ConcentrationMacaca mulattaMolecular Sequence DataMutagenesis, Site-DirectedNef Gene Products, Human Immunodeficiency VirusPhosphorylationProtein Kinase CSimian immunodeficiency virusSurface PropertiesVirus ReplicationConceptsProtein kinase C phosphorylation sitesKinase C phosphorylation sitesC phosphorylation sitesPhosphorylation sitesPutative protein kinase C phosphorylation sitePotential protein kinase C phosphorylation sitesAcidic regionWild-type activityPKC phosphorylation sitesJurkat T cellsSignal transductionAbility of NefReplication assaysFunctional relevanceCell culture systemMutant virusNef functionPathogenic SIVmac239 cloneAcidic chargeReduced infectivityReplicationCulture systemNefRapid reversionRhesus macaques
1997
Association of simian immunodeficiency virus Nef with cellular serine/threonine kinases is dispensable for the development of AIDS in rhesus macaques
Lang S, Lafrate A, Stahl-Hennig C, Kuhn E, Nisslein T, Kaup F, Haupt M, Hunsmann G, Skowronski J, Kirchhoff F. Association of simian immunodeficiency virus Nef with cellular serine/threonine kinases is dispensable for the development of AIDS in rhesus macaques. Nature Medicine 1997, 3: 860-865. PMID: 9256276, DOI: 10.1038/nm0897-860.Peer-Reviewed Original ResearchConceptsCellular serine/threonine kinaseSerine/threonine kinaseThreonine kinaseTyrosine kinase SrcPutative SH3Simian immunodeficiency virus NefKinase SrcMutant formsEfficient replicationKinaseInduction of AIDSSimian immunodeficiency virusNef geneCD4 downregulationSIVmac NefRhesus macaquesRapid deathNef sequencesSH3NefSrcGenesDomainDifferent time pointsPathogenicity
1995
Identification of a nef allele that causes lymphocyte activation and acute disease in Macaque monkeys
Du Z, Lang S, Sasseville V, Lackner A, Ilyinskii P, Daniel M, Jung J, Desrosiers R. Identification of a nef allele that causes lymphocyte activation and acute disease in Macaque monkeys. Cell 1995, 82: 665-674. PMID: 7664345, DOI: 10.1016/0092-8674(95)90038-1.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAllelesAmino Acid SequenceAnimalsBase SequenceDNA PrimersDNA, ViralGene Products, envGene Products, nefGenes, nefLymphocyte ActivationMacaca mulattaMiceMolecular Sequence DataPhagocytesPhenotypePhosphorylationRetroviridae Proteins, OncogenicSignal TransductionSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusTransformation, GeneticTyrosineViral Fusion ProteinsVirus ReplicationConceptsAcute diseasePeripheral blood mononuclear cell culturesBlood mononuclear cell culturesMononuclear cell culturesT lymphocyte activationSevere diarrheaLymphoid proliferationsGastrointestinal tractNef allelesMacaque monkeysLymphocyte activationCellular activationSIVmac239NefDiseaseActivationMonkeysCell culturesImportant determinantCellsNIH 3T3 cellsTyrosine phosphorylationCotransfected COS cellsCOS cellsDiarrhea
1993
Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus
Lang S, Weeger M, Stahl-Hennig C, Coulibaly C, Hunsmann G, Müller J, Müller-Hermelink H, Fuchs D, Wachter H, Daniel M. Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus. Journal Of Virology 1993, 67: 902-912. PMID: 8380472, PMCID: PMC237444, DOI: 10.1128/jvi.67.2.902-912.1993.Peer-Reviewed Original ResearchConceptsVpr variantsTAA stop codonVpr geneATG start codonSite-specific mutagenesisVpr mutant virusSignificant selective pressureImportance of VprStart codonSelective pressureVpr mutantsStop codonGenesPathogenic molecular cloneMolecular cloneCell linesVprTest animalsCEMx174 cell lineCodonNef geneSimilar kineticsVirus replicationReversionStop signal