2008
Vaccine Protection by Live, Attenuated Simian Immunodeficiency Virus in the Absence of High-Titer Antibody Responses and High-Frequency Cellular Immune Responses Measurable in the Periphery
Mansfield K, Lang S, Gauduin M, Sanford H, Lifson J, Johnson R, Desrosiers R. Vaccine Protection by Live, Attenuated Simian Immunodeficiency Virus in the Absence of High-Titer Antibody Responses and High-Frequency Cellular Immune Responses Measurable in the Periphery. Journal Of Virology 2008, 82: 4135-4148. PMID: 18272584, PMCID: PMC2292994, DOI: 10.1128/jvi.00015-08.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodies, ViralInterferon-gammaIntestinal MucosaLymphoid TissueMacaca mulattaNeutralization TestsRetroviridae ProteinsRNA, ViralSAIDS VaccinesSequence DeletionSimian Acquired Immunodeficiency SyndromeSimian immunodeficiency virusT-LymphocytesVaccines, AttenuatedViral Envelope ProteinsViral LoadViremiaConceptsAnti-SIV antibody responsesSimian immunodeficiency virusAntibody responseImmune responseVaccine protectionImmunodeficiency virusCopies/Gamma interferon enzyme-linked immunospotVaccine/challenge experimentsAttenuated simian immunodeficiency virusGut-associated lymphoid tissueHigh-titer antibody responsesAnti-SIV immune responsesEnzyme-linked immunospotMacrophage marker CD68Cellular immune responsesHumoral immune responseViral load measurementsWeeks of infectionNeutralization-resistant strainDouble-labeling experimentsVaccinated monkeysViral loadPeripheral bloodLymphoid tissue
2005
Immunization of Macaques with Single-Cycle Simian Immunodeficiency Virus (SIV) Stimulates Diverse Virus-Specific Immune Responses and Reduces Viral Loads after Challenge with SIVmac239
Evans D, Bricker J, Sanford H, Lang S, Carville A, Richardson B, Piatak M, Lifson J, Mansfield K, Desrosiers R. Immunization of Macaques with Single-Cycle Simian Immunodeficiency Virus (SIV) Stimulates Diverse Virus-Specific Immune Responses and Reduces Viral Loads after Challenge with SIVmac239. Journal Of Virology 2005, 79: 7707-7720. PMID: 15919923, PMCID: PMC1143664, DOI: 10.1128/jvi.79.12.7707-7720.2005.Peer-Reviewed Original ResearchConceptsSingle-Cycle Simian Immunodeficiency VirusesRNA copies/AIDS vaccine approachSingle-cycle SIVEnzyme-linked immunospotViral loadSimian immunodeficiency virusCopies/Immunodeficiency virusVaccine approachesGamma interferon enzyme-linked immunospotGag-specific T-cell responsesVirus-specific immune responsesMajor Histocompatibility Complex Class I DownregulationT cell proliferative responsesImmunization of macaquesPlasma viral loadT cell responsesMHC class IThird doseChronic phasePeripheral bloodWeek 8Immune responseControl animals
2003
Importance of B-Cell Responses for Immunological Control of Variant Strains of Simian Immunodeficiency Virus
Johnson W, Lifson J, Lang S, Johnson R, Desrosiers R. Importance of B-Cell Responses for Immunological Control of Variant Strains of Simian Immunodeficiency Virus. Journal Of Virology 2003, 77: 375-381. PMID: 12477842, PMCID: PMC140644, DOI: 10.1128/jvi.77.1.375-381.2003.Peer-Reviewed Original ResearchConceptsHigh viral loadSimian immunodeficiency virusViral loadAntibody-mediated neutralizationImmunological controlEffective immunological controlImmunodeficiency virusVariant strainsContinuous viral replicationReplicative capacityB cell responsesGp120 envelope proteinTime of infectionHigh replicative capacityAntibody responseDisease progressionB cellsDay 14Tissue macrophagesExperimental infectionViral replicationRhesus monkeysVariable region 1InfectionV2 sequences
2000
Simian Immunodeficiency Virus Containing Mutations in N-Terminal Tyrosine Residues and in the PxxP Motif in Nef Replicates Efficiently in Rhesus Macaques
Carl S, Iafrate A, Lang S, Stolte N, Stahl-Hennig C, Mätz-Rensing K, Fuchs D, Skowronski J, Kirchhoff F. Simian Immunodeficiency Virus Containing Mutations in N-Terminal Tyrosine Residues and in the PxxP Motif in Nef Replicates Efficiently in Rhesus Macaques. Journal Of Virology 2000, 74: 4155-4164. PMID: 10756028, PMCID: PMC111930, DOI: 10.1128/jvi.74.9.4155-4164.2000.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBinding SitesCD4 AntigensCell Line, TransformedCOS CellsDown-RegulationGene Products, nefHistocompatibility Antigens Class IHumansMacaca mulattaMutagenesisP21-Activated KinasesPhosphorylationProtein Serine-Threonine KinasesSimian immunodeficiency virusSrc Homology DomainsTyrosineViral LoadVirus ReplicationConceptsAbility of NefTyrosine-based endocytosis signalTyrosine residuesHigh viral loadCellular signal transductionTyrosine kinase SrcNef functionViral loadEndocytosis signalEndocytic machineryPXXP motifN-terminal tyrosine residueCell surface expressionKinase SrcKinase assaysSignal transductionProline residuesClass I major histocompatibility complexN-terminal tyrosineI major histocompatibility complexLigand domainCombined mutationsNef interactionsMajor histocompatibility complexMutations