2021
Deletion of a putative promoter-proximal Tnfsf11 regulatory region in mice does not alter bone mass or Tnfsf11 expression in vivo
MacLeod RS, Meyer MB, Xiong J, Cawley KM, Liu Y, Onal M, Benkusky NA, Thostenson JD, Pike JW, O’Brien C. Deletion of a putative promoter-proximal Tnfsf11 regulatory region in mice does not alter bone mass or Tnfsf11 expression in vivo. PLOS ONE 2021, 16: e0250974. PMID: 33970941, PMCID: PMC8109787, DOI: 10.1371/journal.pone.0250974.Peer-Reviewed Original ResearchConceptsMonths of ageParathyroid hormoneBone massVitamin D3Osteoclast formationLumbar vertebraeElevated parathyroid hormoneSympathetic nervous systemPathological bone resorptionCultured stromal cellsWild-type littermatesFourth lumbar vertebraFifth lumbar vertebraMRNA abundanceLymph nodesLymphocyte productionBone resorptionCytokine RANKLT lymphocytesKnockout miceBone marrowNervous systemStromal cellsNormal physiological conditionsMaximal stimulation
2019
Effective CRISPR interference of an endogenous gene via a single transgene in mice
MacLeod RS, Cawley KM, Gubrij I, Nookaew I, Onal M, O’Brien C. Effective CRISPR interference of an endogenous gene via a single transgene in mice. Scientific Reports 2019, 9: 17312. PMID: 31754144, PMCID: PMC6872636, DOI: 10.1038/s41598-019-53611-6.Peer-Reviewed Original Research