Deletion of a putative promoter-proximal Tnfsf11 regulatory region in mice does not alter bone mass or Tnfsf11 expression in vivo
MacLeod RS, Meyer MB, Xiong J, Cawley KM, Liu Y, Onal M, Benkusky NA, Thostenson JD, Pike JW, O’Brien C. Deletion of a putative promoter-proximal Tnfsf11 regulatory region in mice does not alter bone mass or Tnfsf11 expression in vivo. PLOS ONE 2021, 16: e0250974. PMID: 33970941, PMCID: PMC8109787, DOI: 10.1371/journal.pone.0250974.Peer-Reviewed Original ResearchConceptsMonths of ageParathyroid hormoneBone massVitamin D3Osteoclast formationLumbar vertebraeElevated parathyroid hormoneSympathetic nervous systemPathological bone resorptionCultured stromal cellsWild-type littermatesFourth lumbar vertebraFifth lumbar vertebraMRNA abundanceLymph nodesLymphocyte productionBone resorptionCytokine RANKLT lymphocytesKnockout miceBone marrowNervous systemStromal cellsNormal physiological conditionsMaximal stimulation