Loss of chaperone-mediated autophagy is associated with low vertebral cancellous bone mass
Akel N, MacLeod R, Berryhill S, Laster D, Dimori M, Crawford J, Fu Q, Onal M. Loss of chaperone-mediated autophagy is associated with low vertebral cancellous bone mass. Scientific Reports 2022, 12: 3134. PMID: 35210514, PMCID: PMC8873216, DOI: 10.1038/s41598-022-07157-9.Peer-Reviewed Original ResearchConceptsProtein degradation pathwaysRole of CMACRISPR-Cas9-based genome editingChaperone-mediated autophagySkeletal homeostasisDegradation pathwayProteome remodelingMouse genomeGene trapCytoplasmic proteinsOsteoblastic cell lineGenome editingProteasomal degradationSpecific knockdownWild-type controlsCell typesLAMP2ACultured cellsOsteoblast formationCell linesRANKL expressionCathepsin K.Pathophysiological conditionsAutophagyCMA process