Ryan MacLeod, MD, PhD
he/him/his
Clinical FellowAbout
Research
Publications
2023
A Ticklish Parrot-dox: Dual Diagnosis of Lyme Carditis and Psittacosis
Tran P, Maayah M, MacLeod R, Frampton J. A Ticklish Parrot-dox: Dual Diagnosis of Lyme Carditis and Psittacosis. Journal Of Brown Hospital Medicine 2023, 2 DOI: 10.56305/001c.72765.Peer-Reviewed Original ResearchLyme carditisDry coughDiagnosis of Lyme carditisComplete heart blockInitiation of treatmentShortness of breathSymptoms of dry coughClinical suspicionHeart blockAtrial fibrillationAtrial flutterChest pressureEarly treatmentCarditisEndemic areasYoung femalesHeadacheCoughYoung individualsLyme diseasePsittacosis
2022
Loss of chaperone-mediated autophagy is associated with low vertebral cancellous bone mass
Akel N, MacLeod R, Berryhill S, Laster D, Dimori M, Crawford J, Fu Q, Onal M. Loss of chaperone-mediated autophagy is associated with low vertebral cancellous bone mass. Scientific Reports 2022, 12: 3134. PMID: 35210514, PMCID: PMC8873216, DOI: 10.1038/s41598-022-07157-9.Peer-Reviewed Original ResearchConceptsProtein degradation pathwaysRole of CMACRISPR-Cas9-based genome editingChaperone-mediated autophagySkeletal homeostasisDegradation pathwayProteome remodelingMouse genomeGene trapCytoplasmic proteinsOsteoblastic cell lineGenome editingProteasomal degradationSpecific knockdownWild-type controlsCell typesLAMP2ACultured cellsOsteoblast formationCell linesRANKL expressionCathepsin K.Pathophysiological conditionsAutophagyCMA process
2021
Deletion of a putative promoter-proximal Tnfsf11 regulatory region in mice does not alter bone mass or Tnfsf11 expression in vivo
MacLeod RS, Meyer MB, Xiong J, Cawley KM, Liu Y, Onal M, Benkusky NA, Thostenson JD, Pike JW, O’Brien C. Deletion of a putative promoter-proximal Tnfsf11 regulatory region in mice does not alter bone mass or Tnfsf11 expression in vivo. PLOS ONE 2021, 16: e0250974. PMID: 33970941, PMCID: PMC8109787, DOI: 10.1371/journal.pone.0250974.Peer-Reviewed Original ResearchConceptsMonths of ageParathyroid hormoneBone massVitamin D3Osteoclast formationLumbar vertebraeElevated parathyroid hormoneSympathetic nervous systemPathological bone resorptionCultured stromal cellsWild-type littermatesFourth lumbar vertebraFifth lumbar vertebraMRNA abundanceLymph nodesLymphocyte productionBone resorptionCytokine RANKLT lymphocytesKnockout miceBone marrowNervous systemStromal cellsNormal physiological conditionsMaximal stimulation
2020
Osteocyte RANKL is required for cortical bone loss with age and is induced by senescence
Kim HN, Xiong J, MacLeod RS, Iyer S, Fujiwara Y, Cawley KM, Han L, He Y, Thostenson JD, Ferreira E, Jilka RL, Zhou D, Almeida M, O'Brien CA. Osteocyte RANKL is required for cortical bone loss with age and is induced by senescence. JCI Insight 2020, 5: e138815. PMID: 32870816, PMCID: PMC7566701, DOI: 10.1172/jci.insight.138815.Peer-Reviewed Original ResearchConceptsCortical bone lossBone lossControl miceAged miceCortical boneAge-associated bone lossOsteoclastogenic cytokine RANKLOsteocyte-derived RANKLTrabecular boneMonths of ageSenescent cellsConditional knockout miceHuman cell culture modelsRANKL levelsCytokine RANKLRANKL productionKnockout miceTranscription factor GATA4Cell culture modelSenolytic compoundsRANKLMiceAgeBoneCellular senescenceLocal Production of Osteoprotegerin by Osteoblasts Suppresses Bone Resorption
Cawley KM, Bustamante-Gomez NC, Guha AG, MacLeod RS, Xiong J, Gubrij I, Liu Y, Mulkey R, Palmieri M, Thostenson JD, Goellner JJ, O’Brien C. Local Production of Osteoprotegerin by Osteoblasts Suppresses Bone Resorption. Cell Reports 2020, 32: 108052. PMID: 32905775, PMCID: PMC7493998, DOI: 10.1016/j.celrep.2020.108052.Peer-Reviewed Original Research
2019
Effective CRISPR interference of an endogenous gene via a single transgene in mice
MacLeod RS, Cawley KM, Gubrij I, Nookaew I, Onal M, O’Brien C. Effective CRISPR interference of an endogenous gene via a single transgene in mice. Scientific Reports 2019, 9: 17312. PMID: 31754144, PMCID: PMC6872636, DOI: 10.1038/s41598-019-53611-6.Peer-Reviewed Original Research