Genome-wide methylation and expression profiling identifies promoter characteristics affecting demethylation-induced gene up-regulation in melanoma
Rubinstein JC, Tran N, Ma S, Halaban R, Krauthammer M. Genome-wide methylation and expression profiling identifies promoter characteristics affecting demethylation-induced gene up-regulation in melanoma. BMC Medical Genomics 2010, 3: 4. PMID: 20144234, PMCID: PMC2843643, DOI: 10.1186/1755-8794-3-4.Peer-Reviewed Original ResearchMeSH KeywordsAgedAntimetabolites, AntineoplasticAzacitidineCell Line, TumorCpG IslandsDecitabineDNA MethylationDNA Modification MethylasesEnzyme InhibitorsEpigenesis, GeneticFemaleGene Expression ProfilingGenome, HumanHumansMaleMelanomaMiddle AgedPromoter Regions, GeneticTumor Suppressor ProteinsUp-RegulationConceptsHigh CpG contentCpG contentGenome-wide gene expression dataMethylation levelsPromoter CpG contentUp-regulates gene expressionGenome-wide methylationGenome-wide dataLow CpG contentMelanoma cell strainsGene expression dataTumor suppressor geneCytosine analog 5Lower methylation levelsTranscriptional silencingMethylated promotersHigher methylation levelsBisulfite sequencingDNA methylationCpG methylationDNA methyltransferaseGenomic hypomethylationPromoter characteristicsSpecific genesGene expression