2001
Characterization of a DNA-Cleaving deoxyribozyme
Carmi N, Breaker R. Characterization of a DNA-Cleaving deoxyribozyme. Bioorganic & Medicinal Chemistry 2001, 9: 2589-2600. PMID: 11557347, DOI: 10.1016/s0968-0896(01)00035-9.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCatalysisCopperDeoxyadenosinesDeoxyribonucleotidesDNADNA, CatalyticDNA, Single-StrandedElectrophoresis, Gel, Two-DimensionalModels, MolecularMolecular StructureNucleic Acid ConformationOxidation-ReductionSequence Analysis, DNAStructure-Activity RelationshipSubstrate Specificity
2000
Tech.Sight. Molecular biology. Making catalytic DNAs.
Breaker R. Tech.Sight. Molecular biology. Making catalytic DNAs. Science 2000, 290: 2095-6. PMID: 11187837, DOI: 10.1126/science.290.5499.2095.Peer-Reviewed Original ResearchAllosteric nucleic acid catalysts
Soukup G, Breaker R. Allosteric nucleic acid catalysts. Current Opinion In Structural Biology 2000, 10: 318-325. PMID: 10851196, DOI: 10.1016/s0959-440x(00)00090-7.Peer-Reviewed Original ResearchStructural diversity of self-cleaving ribozymes
Tang J, Breaker R. Structural diversity of self-cleaving ribozymes. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 5784-5789. PMID: 10823936, PMCID: PMC18511, DOI: 10.1073/pnas.97.11.5784.Peer-Reviewed Original ResearchAltering molecular recognition of RNA aptamers by allosteric selection11Edited by D. E. Draper
Soukup G, Emilsson G, Breaker R. Altering molecular recognition of RNA aptamers by allosteric selection11Edited by D. E. Draper. Journal Of Molecular Biology 2000, 298: 623-632. PMID: 10788325, DOI: 10.1006/jmbi.2000.3704.Peer-Reviewed Original ResearchCapping DNA with DNA †
Li Y, Liu Y, Breaker R. Capping DNA with DNA †. Biochemistry 2000, 39: 3106-3114. PMID: 10715132, DOI: 10.1021/bi992710r.Peer-Reviewed Original ResearchSelection for Catalytic Function with Nucleic Acids
Breaker R. Selection for Catalytic Function with Nucleic Acids. Current Protocols In Nucleic Acid Chemistry 2000, 00: 9.4.1-9.4.17. PMID: 18428882, DOI: 10.1002/0471142700.nc0904s00.Peer-Reviewed Original Research
1999
In vitro selection of deoxyribozymes with DNA capping activity.
Li Y, Liu Y, Breaker R. In vitro selection of deoxyribozymes with DNA capping activity. Nucleic Acids Symposium Series 1999, 42: 237-8. PMID: 10780467, DOI: 10.1093/nass/42.1.237.Peer-Reviewed Original ResearchAllosteric selection of ribozymes that respond to the second messengers cGMP and cAMP
Koizumi M, Soukup G, Kerr J, Breaker R. Allosteric selection of ribozymes that respond to the second messengers cGMP and cAMP. Nature Structural & Molecular Biology 1999, 6: 1062-1071. PMID: 10542100, DOI: 10.1038/14947.Peer-Reviewed Original ResearchConceptsRNA molecular switchGenetic control elementsMolecular recognition characteristicsEmergence of ribozymesSecond messenger cGMPRNAs exhibitAllosteric ribozymesRNA transcriptsCellular RNASelective sensorCAMP additionMolecular switchFold activationCatalytic rateRecognition characteristicsRibozymeControl elementsEffector compoundsHammerhead ribozymeChemical agentsCompoundsStructural characteristicsSpecific nucleosideNew combinatorial strategyCombinatorial strategiesDeoxyribozymes: New players in the ancient game of biocatalysis
Li Y, Breaker R. Deoxyribozymes: New players in the ancient game of biocatalysis. Current Opinion In Structural Biology 1999, 9: 315-323. PMID: 10361095, DOI: 10.1016/s0959-440x(99)80042-6.Peer-Reviewed Original ResearchConceptsSubstrate recognitionGenetic informationIdeal storage systemBiological catalysisRNA counterpartsInert characterChemical reactionsDNANovel chemicalRate enhancementSubstantial untapped potentialNew playersArtificial DNAHelical structurePolynucleotide chainSurprising varietyDeoxyribozymesRecent studiesStructure formationProteinCatalysisBiocatalysisStorage systemReactionChemicals
1998
Cleaving DNA with DNA
Carmi N, Balkhi S, Breaker R. Cleaving DNA with DNA. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 2233-2237. PMID: 9482868, PMCID: PMC19303, DOI: 10.1073/pnas.95.5.2233.Peer-Reviewed Original Research
1997
DNA aptamers and DNA enzymes
Breaker R. DNA aptamers and DNA enzymes. Current Opinion In Chemical Biology 1997, 1: 26-31. PMID: 9667831, DOI: 10.1016/s1367-5931(97)80105-6.Peer-Reviewed Original ResearchDNA enzymes
Breaker R. DNA enzymes. Nature Biotechnology 1997, 15: 427-431. PMID: 9131619, DOI: 10.1038/nbt0597-427.Peer-Reviewed Original Research
1994
A DNA enzyme that cleaves RNA
Breaker R, Joyce G. A DNA enzyme that cleaves RNA. Cell Chemical Biology 1994, 1: 223-229. PMID: 9383394, DOI: 10.1016/1074-5521(94)90014-0.Peer-Reviewed Original ResearchConceptsDNA enzymeRNA enzymeDependent cleavageSimilarity of DNATypes of RNACatalytic domainDNA sequencesRandom nucleotidesRapid turnoverRNASuccessive roundsEnzymeDNACatalytic rateSelective amplificationBiological systemsTurnover rateSequenceCleavageIntermolecular contextNucleotidesPopulationTurnoverPhosphoesters