Engineering precision RNA molecular switches
Soukup G, Breaker R. Engineering precision RNA molecular switches. Proceedings Of The National Academy Of Sciences Of The United States Of America 1999, 96: 3584-3589. PMID: 10097080, PMCID: PMC22337, DOI: 10.1073/pnas.96.7.3584.Peer-Reviewed Original ResearchMeSH KeywordsAllosteric RegulationBase SequenceDNADNA PrimersFlavin MononucleotideKineticsMolecular Sequence DataNucleic Acid ConformationOligoribonucleotidesReverse Transcriptase Polymerase Chain ReactionRNARNA, CatalyticTheophyllineConceptsRNA molecular switchMolecular switchGenetic control elementsEnzyme engineering strategiesRNA switchesReceptor domainConformational changesControl elementsEngineering strategiesStructural bridgeModular natureMolecular sensorsStructural reorganizationCorresponding ligandsRNARibozymeSwitchLigandsCatalyticReceptorsTripartite constructsReorganizationDomain