Production of RNA by a polymerase protein encapsulated within phospholipid vesicles
Chakrabarti A, Breaker R, Joyce G, Deamer D. Production of RNA by a polymerase protein encapsulated within phospholipid vesicles. Journal Of Molecular Evolution 1994, 39: 555-559. PMID: 7528810, DOI: 10.1007/bf00160400.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine DiphosphateDimyristoylphosphatidylcholineDNA-Directed RNA PolymerasesElectrophoresis, Polyacrylamide GelFluorescenceLiposomesRNAConceptsRNA polymeraseTemplate-independent RNA polymeraseProduction of RNACatalyzed polymerization reactionsBoundary membraneMacromolecular catalystsRNA productsPolymerization reactionPolymerase proteinDimyristoyl phosphatidylcholine vesiclesPolyacrylamide gel electrophoresisEthidium bromide fluorescenceEarly cellsRNA polymersPhase transition temperatureSubstrate fluxPhospholipid vesiclesVesiclesFree enzymeSuch reactionsGel electrophoresisPhosphatidylcholine vesiclesComponent lipidsPolymeraseCatalystContinuous in vitro evolution of bacteriophage RNA polymerase promoters.
Breaker R, Banerji A, Joyce G. Continuous in vitro evolution of bacteriophage RNA polymerase promoters. Biochemistry 1994, 33: 11980-6. PMID: 7522554, DOI: 10.1021/bi00205a037.Peer-Reviewed Original Research