Kinetics of RNA Degradation by Specific Base Catalysis of Transesterification Involving the 2‘-Hydroxyl Group
Li Y, Breaker R. Kinetics of RNA Degradation by Specific Base Catalysis of Transesterification Involving the 2‘-Hydroxyl Group. Journal Of The American Chemical Society 1999, 121: 5364-5372. DOI: 10.1021/ja990592p.Peer-Reviewed Original ResearchSpecific base catalysisBase catalysisHydroxyl groupsRNA phosphodiesterRate enhancementMaximum rate enhancementGeneral base catalysisEnzyme mimicsAlkaline pH conditionsHydroxide ionTransfer reactionsReaction conditionsCatalysisBase-catalyzed cleavageDivalent magnesiumRNA polymersDNA/RNARNA linkageTransesterification reactionPH conditionsCleavage mechanismStrand scissionRNA transesterificationBiological systemsDominant pathway