2001
Immobilized RNA switches for the analysis of complex chemical and biological mixtures
Seetharaman S, Zivarts M, Sudarsan N, Breaker R. Immobilized RNA switches for the analysis of complex chemical and biological mixtures. Nature Biotechnology 2001, 19: 336-341. PMID: 11283591, DOI: 10.1038/86723.Peer-Reviewed Original ResearchConceptsDrug analytesMetal ionsBiological mixturesBiosensor arrayAnalyte sensorRNA molecular switchComplex mixturesComplex chemicalMolecular switchEnzyme cofactorMixtureRNA switchesBacterial culture mediumAnalytesMoietyIonsGoldImmobilizationCorresponding effectorsChemicalsStatus of targetAddressable pixelsRibozymeCofactor
1996
In vitro selection of self-cleaving DNAs
Carmi N, Shultz L, Breaker R. In vitro selection of self-cleaving DNAs. Cell Chemical Biology 1996, 3: 1039-1046. PMID: 9000012, DOI: 10.1016/s1074-5521(96)90170-2.Peer-Reviewed Original ResearchConceptsIndividual catalystsCatalytic DNAEnzyme-like activityChemical transformationsSole cofactorRate enhancementAdditional reactionsCu2DNA enzymeCatalystDNA cleavageBiological contextVitro SelectionUncatalyzed rateOxidative mechanismsDNAFurther optimizationDistinct classesRibozymeHydroxylDeoxyribozymesBiocatalystReactionCleavageCofactor