1990
Reductions in γ-GIobin mRNA Levels Restricted to the Cytoplasm of 5-Fluorouridine Treated K-562 Human Erythroleukemia Cells
Heimer R, Sartorelli A. Reductions in γ-GIobin mRNA Levels Restricted to the Cytoplasm of 5-Fluorouridine Treated K-562 Human Erythroleukemia Cells. Oncology Research Featuring Preclinical And Clinical Cancer Therapeutics 1990, 2: 45-53. PMID: 2369550, DOI: 10.3727/095535490820874759.Peer-Reviewed Original ResearchConceptsGamma-globin mRNAHuman erythroleukemia cellsErythroleukemia cellsRNA synthesisRibosomal RNA synthesisNuclease protection analysisMRNA levelsMRNA degradationSteady-state levelsCytoplasmic mRNAMRNA speciesCytoplasmic rRNACytoplasmic endonucleaseMRNA synthesisBeta-actin mRNANorthern blottingProtection analysisUntreated control cellsProtein levelsMRNAControl cellsUntreated cellsUridine analogs
1985
Lack of enhanced cytotoxicity of cultured L1210 cells using folinic acid in combination with sequential methotrexate and fluorouracil
Danhauser L, Heimer R, Cadman E. Lack of enhanced cytotoxicity of cultured L1210 cells using folinic acid in combination with sequential methotrexate and fluorouracil. Cancer Chemotherapy And Pharmacology 1985, 15: 214-219. PMID: 3876889, DOI: 10.1007/bf00263888.Peer-Reviewed Original ResearchConceptsSequential methotrexateΜM methotrexateL1210 cellsSynergistic cell killingCultured L1210 cellsFolinic acidMTX pretreatmentMethotrexateL1210 cell growthAcid-precipitable materialHuman neoplasmsPresence of methotrexateFUraInhibitory effectElevated levelsEnhanced cytotoxicityCell killingCell fractionCytotoxicityDUrd incorporationInhibitionThymidylate synthetaseCell growthThymidylate synthesisCultured cells
1981
The influence of methotrexate pretreatment on 5-fluorouracil metabolism in L1210 cells.
Cadman E, Heimer R, Benz C. The influence of methotrexate pretreatment on 5-fluorouracil metabolism in L1210 cells. Journal Of Biological Chemistry 1981, 256: 1695-1704. PMID: 6161926, DOI: 10.1016/s0021-9258(19)69863-7.Peer-Reviewed Original ResearchConceptsMethotrexate pretreatmentConversion of FUraAddition of leucovorinSynergistic antitumor activityAbility of methotrexateTimes control valuesL1210 cellsEnhancement of intracellularSynergistic cell killingSoft agar cloningMethotrexate treatmentAntipurine effectMethotrexateConcentrations of methotrexateMaximal inhibitionControl valuesIntracellular methotrexateFUraInhibitory effectAntitumor activityExact mechanismIntracellular accumulationAgar cloningSimilar elevationsMicroM methotrexate