Effect of de novo purine synthesis inhibitors on 5-fluorouracil metabolism and cytotoxicity
Cadman E, Benz C, Heimer R, O'Shaughnessy J. Effect of de novo purine synthesis inhibitors on 5-fluorouracil metabolism and cytotoxicity. Biochemical Pharmacology 1981, 30: 2469-2472. PMID: 21043247, DOI: 10.1016/0006-2952(81)90343-9.Peer-Reviewed Original ResearchConceptsSynergistic cytotoxicityEffect of methotrexateActivity of methotrexatePurine synthesis inhibitorsMethotrexate pretreatmentPurine synthesisControl levelsIntracellular elevationL-alanosineIntracellular accumulationGlutamine antagonistMethotrexateL1210 cellsSynthesis inhibitorInhibitorsCytotoxicityDe novo purineL-norleucineMetabolismAntagonistThe influence of methotrexate pretreatment on 5-fluorouracil metabolism in L1210 cells.
Cadman E, Heimer R, Benz C. The influence of methotrexate pretreatment on 5-fluorouracil metabolism in L1210 cells. Journal Of Biological Chemistry 1981, 256: 1695-1704. PMID: 6161926, DOI: 10.1016/s0021-9258(19)69863-7.Peer-Reviewed Original ResearchConceptsMethotrexate pretreatmentConversion of FUraAddition of leucovorinSynergistic antitumor activityAbility of methotrexateTimes control valuesL1210 cellsEnhancement of intracellularSynergistic cell killingSoft agar cloningMethotrexate treatmentAntipurine effectMethotrexateConcentrations of methotrexateMaximal inhibitionControl valuesIntracellular methotrexateFUraInhibitory effectAntitumor activityExact mechanismIntracellular accumulationAgar cloningSimilar elevationsMicroM methotrexate