2004
Sphingosine-1-phosphate mediates calcium signaling in guinea pig enteroglial cells
Segura BJ, Zhang W, Xiao L, Turner D, Cowles RA, Logsdon C, Mulholland MW. Sphingosine-1-phosphate mediates calcium signaling in guinea pig enteroglial cells. Journal Of Surgical Research 2004, 116: 42-54. PMID: 14732348, DOI: 10.1016/s0022-4804(03)00281-6.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCalciumCalcium ChannelsCalcium SignalingCulture TechniquesDose-Response Relationship, DrugDrug Administration ScheduleEnzyme ActivationGuinea PigsImmunohistochemistryInositol 1,4,5-Trisphosphate ReceptorsLysophospholipidsMyenteric PlexusNeurogliaPertussis ToxinReceptors, Cytoplasmic and NuclearReceptors, G-Protein-CoupledRNA, MessengerSphingolipidsSphingosineType C PhospholipasesConceptsSignal transduction cascadeEnteric nervous systemEnteric gliaLipid lysophosphatidic acidMajor cell typesRT-PCR analysisTransduction cascadeGlial cellsNervous systemEDG-1Trisphosphate receptorCalcium signalingLysophosphatidic acidCell typesExtracellular receptorsPhospholipase CEdg-3Enteric glial cellsBioactive lipidsEdg-5S1P effectsSphingomyelin metabolitesDigestive activityImmunocytochemical analysisEnteric neurons
2001
Cholinergic stimulation of rat acinar cells increases c-fos and c-jun expression via a mitogen-activated protein kinase-dependent pathway
Turner DJ, Cowles RA, Segura BJ, Mulholland MW. Cholinergic stimulation of rat acinar cells increases c-fos and c-jun expression via a mitogen-activated protein kinase-dependent pathway. Journal Of Gastrointestinal Surgery 2001, 5: 661-672. PMID: 12086906, DOI: 10.1016/s1091-255x(01)80110-4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBlotting, WesternCalciumCalcium SignalingCarbacholCells, CulturedCholinergic AgonistsDiaminesDose-Response Relationship, DrugEnzyme ActivationGene ExpressionMolecular Sequence DataPancreasPiperidinesPirenzepinePolymerase Chain ReactionProto-Oncogene Proteins c-fosProto-Oncogene Proteins c-junRatsReceptors, MuscarinicSensitivity and SpecificityConceptsC-jun early response geneMitogen-activated protein kinase activityProtein kinase-dependent pathwayMitogen-activated protein kinase-dependent pathwayProtein kinase activityAR42J acinar cellsEarly response genesKinase-dependent pathwayDose-dependent increaseC-fosAcetylcholine analogue carbacholC-jun expressionAcinar cellsKinase activityGene expressionM2 inhibitionRat acinar cellsMuscarinic receptor subtypes M1Enzyme activationSpecific inhibitorM3 receptor subtypePolymerase chain reaction analysisChain reaction analysisPancreatic exocrine functionIntracellular calcium releaseFunctional overlap of IP3- and cADP-ribose-sensitive calcium stores in guinea pig myenteric neurons
Turner D, Segura B, Cowles R, Zhang W, Mulholland M. Functional overlap of IP3- and cADP-ribose-sensitive calcium stores in guinea pig myenteric neurons. AJP Gastrointestinal And Liver Physiology 2001, 281: g208-g215. PMID: 11408274, DOI: 10.1152/ajpgi.2001.281.1.g208.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine Diphosphate RiboseAdenosine TriphosphateAnesthetics, LocalAnimalsBiological TransportCaffeineCalciumCalcium Channel BlockersCinnarizineCyclic ADP-RiboseDantroleneGuinea PigsInositol 1,4,5-TrisphosphateMicroinjectionsMuscle Relaxants, CentralMyenteric PlexusNeuronsPhosphodiesterase InhibitorsProcaineRyanodineConceptsMyenteric neuronsReceptor subtypesGuinea pig myenteric neuronsDifferent receptor subtypesMobilization of intracellularSuperfusion techniqueCalcium storesRyR antagonistSubsequent perfusionATP exposureNeuronsRyanodine receptorCADP-riboseAntagonistIntracellular microinjectionPerfusionMicroM ATPSubtypesRyRsIntracellularReceptorsTrisphosphate receptorCinnarizineSpecific ligandsMicroinjection