2022
Jejunoileal mucosal growth in mice with a limited microbiome
Shaughnessy MP, Park CJ, Salvi PS, Cowles RA. Jejunoileal mucosal growth in mice with a limited microbiome. PLOS ONE 2022, 17: e0266251. PMID: 35349599, PMCID: PMC8963542, DOI: 10.1371/journal.pone.0266251.Peer-Reviewed Original ResearchConceptsIntestinal mucosal growthMucosal surface areaSmall intestinal mucosal growthCrypt proliferation indexCONV miceGF miceMucosal growthVillus heightEnteral antibioticsCrypt depthCell proportionSmall intestinal villus heightGerm-free miceBroad-spectrum antibioticsConventional floraIntestinal villus heightGerm-free conditionsEnteral administrationMucosal homeostasisGnotobiotic miceProliferation indexEnterocyte proliferationMiceTaller villiGF conditions
2019
Potentiated serotonin signaling in serotonin re‐uptake transporter knockout mice increases enterocyte mass and small intestinal absorptive function
Greig CJ, Zhang L, Cowles RA. Potentiated serotonin signaling in serotonin re‐uptake transporter knockout mice increases enterocyte mass and small intestinal absorptive function. Physiological Reports 2019, 7: e14278. PMID: 31724827, PMCID: PMC6854605, DOI: 10.14814/phy2.14278.Peer-Reviewed Original ResearchConceptsIntestinal absorptive functionEnterocyte massCrypt proliferation indexSmall intestineAbsorptive functionDistal bowelSmall bowelCrypt depthSERT knockoutVillus heightProliferative parametersSmall intestinal absorptive functionPlasma citrulline levelsCrypt cell proliferationPeptide absorptionSignificant increaseMucosal surface areaAttractive therapeutic targetFunctional intestinal mucosaWT miceCitrulline levelsPlasma citrullineSerotonin systemIntestinal mucosaLuminal instillation
2017
Muscarinic acetylcholine receptors participate in small intestinal mucosal homeostasis
Greig CJ, Cowles RA. Muscarinic acetylcholine receptors participate in small intestinal mucosal homeostasis. Journal Of Pediatric Surgery 2017, 52: 1031-1034. PMID: 28359586, DOI: 10.1016/j.jpedsurg.2017.03.037.Peer-Reviewed Original ResearchConceptsCrypt proliferation indexIntestinal mucosal homeostasisMuscarinic acetylcholine receptorsMucosal homeostasisIleal mucosaAcetylcholine receptorsDistal ileal segmentCholinergic nervous systemWild-type miceCrypt cell proliferationCrypt base stem cellsFunctional mucosaMAChR subtypesMuscarinic signalingIleal segmentCrypt depthProliferation indexNervous systemVillus heightMucosal morphometryMucosaHistologic sectionsMiceMouse linesEpithelial cell types
2016
Enhanced serotonin signaling stimulates ordered intestinal mucosal growth
Tackett JJ, Gandotra N, Bamdad MC, Muise ED, Cowles RA. Enhanced serotonin signaling stimulates ordered intestinal mucosal growth. Journal Of Surgical Research 2016, 208: 198-203. PMID: 27993209, DOI: 10.1016/j.jss.2016.09.036.Peer-Reviewed Original ResearchConceptsWild-type littermatesCrypt proliferation indexSerotonin reuptake transporterEnterocyte heightCrypt depth