1998
Chronic hyperosmolality increases NHE3 activity in OKP cells.
Ambühl P, Amemiya M, Preisig PA, Moe OW, Alpern RJ. Chronic hyperosmolality increases NHE3 activity in OKP cells. Journal Of Clinical Investigation 1998, 101: 170-177. PMID: 9421479, PMCID: PMC508553, DOI: 10.1172/jci62.Peer-Reviewed Original ResearchConceptsNa/H exchanger 3Na/H antiporter activityDiabetes mellitusNHE3 mRNA abundanceNHE3 protein abundanceAntiporter activityChronic hypertonicityGlomerular hyperfiltrationDiabetic nephropathyNa/H antiporterChronic hyperosmolalityOKP cellsExchanger 3MOsm/NHE3 activityRelevant increaseMellitusSignificant increaseChronicH antiporterExtracellular glucoseHypertonic incubationMRNA abundanceHypertonicitySmall increase
1995
Acid incubation increases NHE-3 mRNA abundance in OKP cells
Amemiya M, Yamaji Y, Cano A, Moe OW, Alpern RJ. Acid incubation increases NHE-3 mRNA abundance in OKP cells. American Journal Of Physiology 1995, 269: c126-c133. PMID: 7631739, DOI: 10.1152/ajpcell.1995.269.1.c126.Peer-Reviewed Original Research
1993
Glucocorticoids stimulate Na+/H+ antiporter in OKP cells
Baum M, Cano A, Alpern RJ. Glucocorticoids stimulate Na+/H+ antiporter in OKP cells. American Journal Of Physiology 1993, 264: f1027-f1031. PMID: 8391752, DOI: 10.1152/ajprenal.1993.264.6.f1027.Peer-Reviewed Original ResearchConceptsOKP cellsProximal tubule acidificationEffect of dexamethasoneAntiporter activityTime-dependent stimulationM aldosteroneHemodynamic changesTubule acidificationSystemic administrationDependent pH recoveryDexamethasoneM dexamethasoneAcid loadStimulatory effectGlucocorticoidsPH recoveryStimulationPH-sensitive dyePresent studyMaximal velocityCellsDirect effectAldosteronePrevious studiesActivity
1992
Induction of stress proteins in cultured myogenic cells. Molecular signals for the activation of heat shock transcription factor during ischemia.
Benjamin IJ, Horie S, Greenberg ML, Alpern RJ, Williams RS. Induction of stress proteins in cultured myogenic cells. Molecular signals for the activation of heat shock transcription factor during ischemia. Journal Of Clinical Investigation 1992, 89: 1685-1689. PMID: 1569208, PMCID: PMC443048, DOI: 10.1172/jci115768.Peer-Reviewed Original ResearchConceptsGlucose deprivationControl levelsATP depletionHeat shock protein 70Ischemic injuryHeat shock factorIschemic tissueIschemic cellsHigh-pressure liquid chromatographyHypoxia resultsM rotenoneC2 myogenic cellsStress proteinsMyogenic cellsNormoxic conditionsOxidative metabolismProtein 70ATP storesCultured myogenic cellsConsensus heat shock elementIschemiaATP poolHSF activationMajor stress proteinsDNA-binding activity
1991
Differential regulation of Na/H antiporter by acid in renal epithelial cells and fibroblasts.
Moe OW, Miller RT, Horie S, Cano A, Preisig PA, Alpern RJ. Differential regulation of Na/H antiporter by acid in renal epithelial cells and fibroblasts. Journal Of Clinical Investigation 1991, 88: 1703-1708. PMID: 1658050, PMCID: PMC295708, DOI: 10.1172/jci115487.Peer-Reviewed Original ResearchConceptsNa/H antiporter activityRenal tubule cellsNa/H antiporterMCT cellsRenal epithelial cellsTubule cellsCell linesCultured renal tubule cellsEpithelial cellsMRNA abundanceOpossum kidney cell lineChronic metabolic acidosisAcid preincubationTubule cell lineRat renal cortexH antiporterAntiporter activityUnderlying molecular mechanismsDifferential regulationMetabolic acidosisRenal cortexAcidotic environmentAcid feedingAdaptive increaseKidney cell lineInhibition of Na(+)-independent H+ pump by Na(+)-induced changes in cell Ca2+.
Hays SR, Alpern RJ. Inhibition of Na(+)-independent H+ pump by Na(+)-induced changes in cell Ca2+. The Journal Of General Physiology 1991, 98: 791-813. PMID: 1660062, PMCID: PMC2229082, DOI: 10.1085/jgp.98.4.791.Peer-Reviewed Original ResearchConceptsCell pH recoveryCell Ca2PH recoveryAcid loadPump activityRenal outer medullaryDelayed activationCell acidificationRapid cell acidificationUnits/minApical membraneCell pHSustained elevationFura-2Outer medullaryExtracellular Ca2Inner stripeIntracellular Ca2Steady-state cell pHSustained increaseDimethyl-BAPTAInitial suppressionSpike increasePhysiologic ratesMicrofilament-disrupting agent
1990
Apical and basolateral membrane H+ extrusion mechanisms in inner stripe of rabbit outer medullary collecting duct
Hays SR, Alpern RJ. Apical and basolateral membrane H+ extrusion mechanisms in inner stripe of rabbit outer medullary collecting duct. American Journal Of Physiology 1990, 259: f628-f635. PMID: 2171359, DOI: 10.1152/ajprenal.1990.259.4.f628.Peer-Reviewed Original ResearchConceptsUnits/minPHi recoveryInner stripeAcid loadInitial control levelsSCH 28080Luminal Sch-28080ATPase inhibitorBasolateral membraneTriphasic responseExtrusion mechanismSlow alkalinizationRate of alkalinizationControl levelsCell acidificationRabbitsInhibitorsDuctTotal absenceInitial rateAlkalinizationN-ethylmaleimideRecoveryMinPathways for apical and basolateral membrane NH3 and NH4+ movement in rat proximal tubule
Preisig PA, Alpern RJ. Pathways for apical and basolateral membrane NH3 and NH4+ movement in rat proximal tubule. American Journal Of Physiology 1990, 259: f587-f593. PMID: 2221097, DOI: 10.1152/ajprenal.1990.259.4.f587.Peer-Reviewed Original ResearchPreincubation in acid medium increases Na/H antiporter activity in cultured renal proximal tubule cells.
Horie S, Moe O, Tejedor A, Alpern RJ. Preincubation in acid medium increases Na/H antiporter activity in cultured renal proximal tubule cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1990, 87: 4742-4745. PMID: 2162055, PMCID: PMC54193, DOI: 10.1073/pnas.87.12.4742.Peer-Reviewed Original ResearchConceptsNa/H antiporter activityProximal tubule cellsTubule cellsCultured renal proximal tubule cellsCultured proximal tubule cellsRenal proximal tubule cellsPreincubation of fibroblastsExtracellular fluidAntiporter activityChronic acidosisAcidotic environmentCO2/HCO3Small inhibitionPreincubationPreincubation mediumAddition of cycloheximideCell acidificationAcid preincubationPresent studyProtein synthesisCellsAcidosisActivityEpithelium
1988
Regulation of cell pH by ambient bicarbonate, carbon dioxide tension, and pH in the rabbit proximal convoluted tubule.
Krapf R, Berry CA, Alpern RJ, Rector FC. Regulation of cell pH by ambient bicarbonate, carbon dioxide tension, and pH in the rabbit proximal convoluted tubule. Journal Of Clinical Investigation 1988, 81: 381-389. PMID: 3123519, PMCID: PMC329580, DOI: 10.1172/jci113330.Peer-Reviewed Original Research
1987
Basolateral membrane Na/base cotransport is dependent on CO2/HCO3 in the proximal convoluted tubule.
Krapf R, Alpern RJ, Rector FC, Berry CA. Basolateral membrane Na/base cotransport is dependent on CO2/HCO3 in the proximal convoluted tubule. The Journal Of General Physiology 1987, 90: 833-853. PMID: 2831294, PMCID: PMC2228880, DOI: 10.1085/jgp.90.6.833.Peer-Reviewed Original ResearchRole of the Na+/H+ antiporter in rat proximal tubule bicarbonate absorption.
Preisig PA, Ives HE, Cragoe EJ, Alpern RJ, Rector FC. Role of the Na+/H+ antiporter in rat proximal tubule bicarbonate absorption. Journal Of Clinical Investigation 1987, 80: 970-978. PMID: 2888788, PMCID: PMC442334, DOI: 10.1172/jci113190.Peer-Reviewed Original Research
1986
Cell pH in the rat proximal convoluted tubule. Regulation by luminal and peritubular pH and sodium concentration.
Alpern RJ, Chambers M. Cell pH in the rat proximal convoluted tubule. Regulation by luminal and peritubular pH and sodium concentration. Journal Of Clinical Investigation 1986, 78: 502-510. PMID: 3016029, PMCID: PMC423587, DOI: 10.1172/jci112602.Peer-Reviewed Original Research
1985
Amiloride inhibition of proximal tubular acidification
Howlin KJ, Alpern RJ, Rector FC. Amiloride inhibition of proximal tubular acidification. American Journal Of Physiology 1985, 248: f773-f778. PMID: 2988347, DOI: 10.1152/ajprenal.1985.248.6.f773.Peer-Reviewed Original ResearchConceptsProximal tubular acidificationLuminal amilorideBicarbonate absorptionX minTubular acidificationM amilorideSodium chloride absorptionLate proximal tubular fluidProximal tubular fluidPerfusion rateProximal acidificationVivo microperfusionTubular fluidKidney cortexChloride absorptionInhibition of acidificationPmol xAmilorideNonspecific inhibitionAmiloride inhibitionGreater inhibitionBrush border membrane vesiclesInhibitionGlucose effluxPotent inhibitor
1984
Increased Vmax for Na+/H+ antiporter activity in proximal tubule brush border vesicles from rabbits with metabolic acidosis
Tsai CJ, Ives HE, Alpern RJ, Yee VJ, Warnock DG, Rector FC. Increased Vmax for Na+/H+ antiporter activity in proximal tubule brush border vesicles from rabbits with metabolic acidosis. American Journal Of Physiology 1984, 247: f339-f343. PMID: 6087681, DOI: 10.1152/ajprenal.1984.247.2.f339.Peer-Reviewed Original Research